FastQCFastQC Report
Thu 2 Feb 2017
SRR4062241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966390
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20000.10170922350093319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25500.027.0965841
GGTATCA10700.025.9126511
CGCAATA4700.019.19357536
GTATACG708.112923E-418.861591
TACCGTA1203.1667696E-718.3320647
ATCAACG37550.018.2788243
TCAACGC37600.018.2540534
TACGAAT4950.018.22512841
ACCGTAT1355.5351848E-817.9246838
CAACGCA38550.017.7471475
TATCAAC38600.017.7246112
AACGCAG38950.017.6213726
CGTAACC750.001291640817.5992283
GCGCAAT5100.017.25458335
ATACGAA5000.017.16230240
CTAGCGG4950.016.88857729
TAGGACG2350.016.8498954
TAGCGGC5100.016.8232230
CGAATGC5000.016.72139243
TCTAGCG5450.016.1464928
Produced by FastQC (version 0.11.5)