FastQCFastQC Report
Thu 2 Feb 2017
SRR4062240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135814
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16831.2391947810976778No Hit
TATCAACGCAGAGTACTTTTTTTTT12080.8894517501877568No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9490.6987497607021368No Hit
GGTATCAACGCAGAGTACTTTTTTT7330.5397087192778358No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6970.5132018790404523No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6930.510256674569632No Hit
GTACATGGGGTGGTATCAACGCAAA5370.39539370020763687No Hit
GTATCAACGCAGAGTACATGGGGTG3420.25181498225514304No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2950.21720882972300354No Hit
GTACATGGGTGGTATCAACGCAAAA2930.2157362274875933No Hit
TATCAACGCAGAGTACATGGGGTGG2360.1737670637784028No Hit
GGTATCAACGCAGAGTACATGGGGT2320.17082185930758242No Hit
GTACATGGGAGTGGTATCAACGCAA2060.15167803024724993No Hit
ATCAACGCAGAGTACTTTTTTTTTT1970.14505132018790404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1540.11339037212658488No Hit
ACGCAGAGTACATGGGGTGGTATCA1510.1111814687734696No Hit
GTATCAACGCAGAGTACATGGGAGT1430.10529105983182882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGAT250.006002329819.00110817
ATAAACG250.006002329819.00110815
ACTCTAA307.670162E-419.00110610
TCTAATT350.00215565416.28666312
CTCTAAT350.00215565416.28666311
CTAATTT350.00215565416.28666313
TGGCAAG653.302941E-614.62162718
TTTTCAA400.005231438714.25608619
CAGAGCG400.00524426814.2508314
AGAGCGA400.00524426814.2508315
AAAGTGT551.9385159E-413.8189866
GTCCTAA551.9506937E-413.8088021
TCCTAAA551.9506937E-413.8088022
CGTGGAA500.001486622113.3007769
ACGTGGA500.001486622113.3007768
CGAAAGC604.034817E-412.67207619
CTAAAGT604.0474083E-412.6674034
AGGACGT853.866413E-612.2948345
GGACGTG853.866413E-612.2948346
CATTTCT853.866413E-612.29483414
Produced by FastQC (version 0.11.5)