##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062240_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135814
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81331821461705	32.0	32.0	32.0	32.0	32.0
2	30.677006788696307	32.0	32.0	32.0	32.0	32.0
3	30.741101800992535	32.0	32.0	32.0	32.0	32.0
4	30.654932481187508	32.0	32.0	32.0	32.0	32.0
5	30.775155727686396	32.0	32.0	32.0	32.0	32.0
6	34.30914338727966	36.0	36.0	36.0	32.0	36.0
7	34.10083643806971	36.0	36.0	36.0	32.0	36.0
8	33.963435286494764	36.0	36.0	36.0	32.0	36.0
9	33.928622969649666	36.0	36.0	36.0	32.0	36.0
10	33.879938739747004	36.0	36.0	36.0	32.0	36.0
11	34.184075279426274	36.0	36.0	36.0	32.0	36.0
12	34.036999131164684	36.0	36.0	36.0	32.0	36.0
13	34.09540989883222	36.0	36.0	36.0	32.0	36.0
14	34.02529194339317	36.0	36.0	36.0	32.0	36.0
15	34.06134124611601	36.0	36.0	36.0	32.0	36.0
16	34.062210081434905	36.0	36.0	36.0	32.0	36.0
17	33.97740291869763	36.0	36.0	36.0	32.0	36.0
18	34.01457876213056	36.0	36.0	36.0	32.0	36.0
19	33.92261475252919	36.0	36.0	36.0	32.0	36.0
20	33.806242360875906	36.0	36.0	36.0	32.0	36.0
21	33.72411533420708	36.0	36.0	36.0	27.0	36.0
22	33.72778211377325	36.0	36.0	36.0	27.0	36.0
23	33.80934955159262	36.0	36.0	36.0	32.0	36.0
24	33.83756461042308	36.0	36.0	36.0	32.0	36.0
25	33.488881853122656	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3.0
5	11.0
6	21.0
7	6.0
8	27.0
9	18.0
10	10.0
11	1.0
12	6.0
13	4.0
14	99.0
15	105.0
16	154.0
17	204.0
18	242.0
19	341.0
20	409.0
21	575.0
22	740.0
23	981.0
24	1161.0
25	1404.0
26	1770.0
27	2140.0
28	2680.0
29	3318.0
30	4293.0
31	5706.0
32	8167.0
33	11809.0
34	26383.0
35	63026.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.3559037493005	17.53158778311195	11.97832297587842	23.13418549170913
2	15.753278404865515	21.01271601393092	39.06989757976041	24.16410800144316
3	18.855199022192114	27.0008982873636	28.734151118441396	25.409751572002886
4	11.79004572867652	15.890899183363894	35.61166707167105	36.70738801628854
5	13.275444294689088	38.97199083791806	31.954602031271868	15.797962836120993
6	31.72160254615645	35.69481485847319	18.06621774942166	14.517364845948693
7	28.772242779849922	32.58981881440639	20.350577169730656	18.287361236013034
8	28.903843035511596	32.474903076492176	19.271194186063653	19.350059701932572
9	28.658199482533668	13.839644407751676	19.640869520348517	37.86128658936614
10	17.219998083062382	27.895866050297496	30.16227614223677	24.721859724403352
11	34.87487280824645	22.72345194732418	22.84363893763549	19.558036306793884
12	25.330099306256958	25.608776107519116	29.134258815549867	19.92686577067406
13	29.939695973342378	21.183816699349777	26.210135204871506	22.666352122436344
14	23.610578765810654	21.001267800807856	27.341746027066073	28.046407406315417
15	23.948241539482414	28.514340485143403	25.253999852539998	22.28341812283418
16	22.566234685302312	27.32982440620992	26.800536659442404	23.303404249045364
17	21.203408822835573	27.536712668082096	27.03688959660297	24.22298891247936
18	21.55566365941388	25.967038150834366	30.281119169762366	22.196179019989387
19	23.784536697890026	25.157529350205248	29.263978657076105	21.793955294828613
20	25.050662854363637	24.015298339732205	28.74923545147051	22.184803354433644
21	25.98070644764284	23.71824633547788	27.92111604872764	22.379931168151636
22	25.18593868630356	24.709021619748935	28.04154412035706	22.06349557359044
23	22.712501381673484	25.469953207324714	29.134519730297338	22.683025680704468
24	22.97646859408509	25.87938772652571	29.431576154645484	21.71256752474372
25	22.812693452819623	26.078177048020518	29.2375379536008	21.87159154555906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	246.0
1	246.0
2	308.5
3	371.0
4	371.0
5	371.0
6	827.0
7	1283.0
8	1283.0
9	1283.0
10	1219.0
11	1155.0
12	1155.0
13	1155.0
14	900.5
15	646.0
16	646.0
17	646.0
18	1057.5
19	1469.0
20	1469.0
21	1469.0
22	2166.5
23	2864.0
24	2864.0
25	2864.0
26	4244.5
27	5625.0
28	5625.0
29	5625.0
30	6924.0
31	8223.0
32	8223.0
33	8223.0
34	8864.5
35	9506.0
36	9506.0
37	9506.0
38	10242.0
39	10978.0
40	10978.0
41	10978.0
42	12440.5
43	13903.0
44	13903.0
45	13903.0
46	16027.5
47	18152.0
48	18152.0
49	18152.0
50	18137.5
51	18123.0
52	18123.0
53	18123.0
54	15898.0
55	13673.0
56	13673.0
57	13673.0
58	12307.0
59	10941.0
60	10941.0
61	10941.0
62	9522.0
63	8103.0
64	8103.0
65	8103.0
66	6652.5
67	5202.0
68	5202.0
69	5202.0
70	3886.5
71	2571.0
72	2571.0
73	2571.0
74	1957.0
75	1343.0
76	1343.0
77	1343.0
78	1083.5
79	824.0
80	824.0
81	824.0
82	577.5
83	331.0
84	331.0
85	331.0
86	237.5
87	144.0
88	144.0
89	144.0
90	101.5
91	59.0
92	59.0
93	59.0
94	34.5
95	10.0
96	10.0
97	10.0
98	39.5
99	69.0
100	69.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014726022354101932
2	7.363011177050966E-4
3	0.0
4	0.009571914530166257
5	0.027243141355088575
6	0.05890408941640773
7	0.11191776989117469
8	0.10308215647871354
9	0.1126540710088798
10	0.1332705023046225
11	0.14136981459937856
12	0.12738009336298173
13	0.12369858777445625
14	0.10749996318494412
15	0.13547940565773778
16	0.11780817883281545
17	0.12222598553904605
18	0.10455475871412373
19	0.09203763971313708
20	0.08320202630067593
21	0.0890924352423167
22	0.1111814687734696
23	0.08025682182985554
24	0.09056503747772689
25	0.0898287363600218
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	135814.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.49893236337932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.62310603994634	58.46967175696173
2	9.077918252920707	12.254995803083629
3	1.8805973405473804	3.8081493807707605
4	0.6774077427377745	1.82897197637946
5	0.3490667917489337	1.1780817883281547
6	0.22907508208523777	0.9277394083084218
7	0.16798839352917433	0.7937326048860943
8	0.10690170497311094	0.5772600762807958
9	0.09272086655831051	0.5632703550443989
>10	0.6981335834978674	9.054294844419573
>50	0.0676316909013559	3.2441427246086563
>100	0.021816674484308355	2.5137320158452
>500	0.005454168621077089	2.657310733797694
>1k	0.002181667448430836	2.1286465312854346
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1683	1.2391947810976778	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1208	0.8894517501877568	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	949	0.6987497607021368	No Hit
GGTATCAACGCAGAGTACTTTTTTT	733	0.5397087192778358	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	697	0.5132018790404523	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	693	0.510256674569632	No Hit
GTACATGGGGTGGTATCAACGCAAA	537	0.39539370020763687	No Hit
GTATCAACGCAGAGTACATGGGGTG	342	0.25181498225514304	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	295	0.21720882972300354	No Hit
GTACATGGGTGGTATCAACGCAAAA	293	0.2157362274875933	No Hit
TATCAACGCAGAGTACATGGGGTGG	236	0.1737670637784028	No Hit
GGTATCAACGCAGAGTACATGGGGT	232	0.17082185930758242	No Hit
GTACATGGGAGTGGTATCAACGCAA	206	0.15167803024724993	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	197	0.14505132018790404	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	154	0.11339037212658488	No Hit
ACGCAGAGTACATGGGGTGGTATCA	151	0.1111814687734696	No Hit
GTATCAACGCAGAGTACATGGGAGT	143	0.10529105983182882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGAT	25	0.0060023298	19.001108	17
ATAAACG	25	0.0060023298	19.001108	15
ACTCTAA	30	7.670162E-4	19.001106	10
TCTAATT	35	0.002155654	16.286663	12
CTCTAAT	35	0.002155654	16.286663	11
CTAATTT	35	0.002155654	16.286663	13
TGGCAAG	65	3.302941E-6	14.621627	18
TTTTCAA	40	0.0052314387	14.256086	19
CAGAGCG	40	0.005244268	14.25083	14
AGAGCGA	40	0.005244268	14.25083	15
AAAGTGT	55	1.9385159E-4	13.818986	6
GTCCTAA	55	1.9506937E-4	13.808802	1
TCCTAAA	55	1.9506937E-4	13.808802	2
CGTGGAA	50	0.0014866221	13.300776	9
ACGTGGA	50	0.0014866221	13.300776	8
CGAAAGC	60	4.034817E-4	12.672076	19
CTAAAGT	60	4.0474083E-4	12.667403	4
AGGACGT	85	3.866413E-6	12.294834	5
GGACGTG	85	3.866413E-6	12.294834	6
CATTTCT	85	3.866413E-6	12.294834	14
>>END_MODULE