FastQCFastQC Report
Thu 2 Feb 2017
SRR4062240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135814
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.525718998041439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.3320718040849986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.2459245733135023No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.14578762130560916No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1940.14284241683478874No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.1310615989515072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1650.12148968442134095No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA1370.10087325312559824No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1360.10013695200789316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG250.00234556635.19970741
AATAGAA250.00234556635.19970736
CGCGGTC300.00572753929.33308844
TCCGAAA300.00572753929.33308821
TTCCGAA300.00572753929.33308820
ACTGTTC300.00572753929.3330888
AACTGCC300.00572753929.3330887
ATACATG1350.027.7136751
ATAGAAC508.2885E-526.3997837
TCCTGCA450.001391908224.444242
TCAGTTC450.001391908224.4442416
AAATTTA651.798182E-523.69210844
TTAGGAC852.3210669E-723.2939223
GATTTCG500.00256889521.99981741
ATTTCGT500.00256889521.99981742
GACGTGA803.5768317E-621.9998157
GTCCTAC1057.590643E-820.9599231
CTTTAGG956.750997E-720.8496071
ATAACGC654.9015076E-420.3075223
GGACGTG1201.4037141E-820.1664986