##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062240_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	135814
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86857761350082	32.0	32.0	32.0	32.0	32.0
2	31.28919699000103	32.0	32.0	32.0	32.0	32.0
3	31.4959577068638	32.0	32.0	32.0	32.0	32.0
4	31.66296552638167	32.0	32.0	32.0	32.0	32.0
5	31.62888951065428	32.0	32.0	32.0	32.0	32.0
6	35.25283107779757	36.0	36.0	36.0	36.0	36.0
7	35.23636002179451	36.0	36.0	36.0	36.0	36.0
8	35.20335164268779	36.0	36.0	36.0	36.0	36.0
9	35.22360728643586	36.0	36.0	36.0	36.0	36.0
10	35.1386675894974	36.0	36.0	36.0	36.0	36.0
11	35.25566583710074	36.0	36.0	36.0	36.0	36.0
12	35.173877508946056	36.0	36.0	36.0	36.0	36.0
13	35.20439719027493	36.0	36.0	36.0	36.0	36.0
14	35.172684701135374	36.0	36.0	36.0	36.0	36.0
15	35.179075794837054	36.0	36.0	36.0	36.0	36.0
16	35.17747065840046	36.0	36.0	36.0	36.0	36.0
17	35.16107323250917	36.0	36.0	36.0	36.0	36.0
18	35.15009498284418	36.0	36.0	36.0	36.0	36.0
19	35.111093112639345	36.0	36.0	36.0	36.0	36.0
20	35.03146214675954	36.0	36.0	36.0	36.0	36.0
21	35.00094246543066	36.0	36.0	36.0	36.0	36.0
22	35.03504057019158	36.0	36.0	36.0	36.0	36.0
23	35.04143902690444	36.0	36.0	36.0	36.0	36.0
24	35.04683611409722	36.0	36.0	36.0	36.0	36.0
25	35.01756078165727	36.0	36.0	36.0	36.0	36.0
26	34.98919109959209	36.0	36.0	36.0	36.0	36.0
27	34.974148467757374	36.0	36.0	36.0	36.0	36.0
28	34.96522449821079	36.0	36.0	36.0	36.0	36.0
29	34.90389061510595	36.0	36.0	36.0	32.0	36.0
30	34.86570603914177	36.0	36.0	36.0	32.0	36.0
31	34.85162059876007	36.0	36.0	36.0	32.0	36.0
32	34.793636885740796	36.0	36.0	36.0	32.0	36.0
33	34.69434668001826	36.0	36.0	36.0	32.0	36.0
34	34.63800491849147	36.0	36.0	36.0	32.0	36.0
35	34.566016758213436	36.0	36.0	36.0	32.0	36.0
36	34.49323339272829	36.0	36.0	36.0	32.0	36.0
37	34.490545893648665	36.0	36.0	36.0	32.0	36.0
38	34.43404214587598	36.0	36.0	36.0	32.0	36.0
39	34.358335664953536	36.0	36.0	36.0	32.0	36.0
40	34.31923071259222	36.0	36.0	36.0	32.0	36.0
41	34.25844905532566	36.0	36.0	36.0	32.0	36.0
42	34.171212098899964	36.0	36.0	36.0	32.0	36.0
43	34.12977307199552	36.0	36.0	36.0	32.0	36.0
44	34.00494794351098	36.0	36.0	36.0	32.0	36.0
45	33.89276510521743	36.0	36.0	36.0	32.0	36.0
46	33.73859837719234	36.0	36.0	36.0	32.0	36.0
47	33.609701503526885	36.0	36.0	36.0	27.0	36.0
48	33.48575257337241	36.0	36.0	36.0	21.0	36.0
49	33.41467006346916	36.0	36.0	36.0	21.0	36.0
50	33.02195649932997	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	4.0
21	10.0
22	25.0
23	58.0
24	154.0
25	290.0
26	645.0
27	1126.0
28	1997.0
29	2815.0
30	3763.0
31	5163.0
32	6969.0
33	10653.0
34	20458.0
35	81680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75273632573693	18.822090950606192	12.728518185701871	23.696654537955013
2	15.122931807638782	21.233073405641832	39.03259773060299	24.6113970561164
3	18.48571197160802	26.78315036115836	29.061282793252484	25.669854873981134
4	11.529739202144109	15.985833566495353	35.490450174503366	36.993977056857176
5	12.778505897771952	38.78171617064515	32.291221818074725	16.14855611350818
6	31.650639845671286	36.443223820813756	17.48347003990752	14.422666293607433
7	27.977969870558262	32.2735505912498	21.046431148482483	18.702048389709454
8	28.700281267026963	32.2021293828324	19.376500213527322	19.72108913661331
9	28.294579351171457	13.31379681034356	19.993520550164195	38.39810328832079
10	16.852459982034254	27.737935706186402	30.755297686541887	24.654306625237457
11	35.03541608376162	22.130266393744385	23.240608479243672	19.593709043250325
12	25.340539266938606	25.329494750173033	29.441736492555997	19.888229490332364
13	30.16331158790699	20.877081891410313	26.470025181498226	22.489581339184472
14	23.438673479906342	20.438246425258075	28.529459407719383	27.593620687116204
15	23.7508651538133	28.142901320924206	25.85079594150824	22.255437583754254
16	22.579576329217378	26.490100358581287	27.35746946168629	23.572853850515045
17	21.101653732310368	26.60035047933203	28.120812287393054	24.177183500964556
18	21.647989161647548	24.334751940153446	31.90466373127954	22.112595166919462
19	23.651464502923115	23.852474708056608	31.234629714167905	21.26143107485237
20	24.872251756078164	22.820916842151767	30.50274640316904	21.804084998601027
21	25.438467315593382	22.8260709499757	29.639801493218666	22.095660241212244
22	24.200745136731115	23.769272681755933	30.239150603030616	21.790831578482337
23	22.225985539046047	24.340642349095084	31.085896888391478	22.347475223467388
24	22.714320425879702	24.49029179828146	30.947700146525005	21.847687629313835
25	22.526396395069728	24.497474487166272	31.214013282872166	21.762115834891837
26	21.770375442704307	25.518175726918628	31.482258693939464	21.2291901364376
27	21.870352099194488	25.021720882972296	31.026992798975066	22.080934218858143
28	21.356149354617816	25.18573605967116	31.203658024754986	22.254456560956033
29	21.53344328925279	24.968338585691985	31.067210555768266	22.431007569286958
30	21.38029040143728	25.462403911289133	31.14378699967602	22.01351868759756
31	22.3923895916474	25.08062497238871	30.268602647738817	22.258382788225074
32	21.621800724530967	25.82540570789032	30.39422142373281	22.158572143845905
33	21.509722927849324	25.476942560726883	31.213506807153955	21.79982770426984
34	22.12102023385268	25.349011869348804	30.67843784054428	21.851530056254234
35	22.49031764030218	25.612970680489493	30.534407351230357	21.36230432797797
36	21.609860617172142	26.324431387275148	30.358654915214302	21.707053080338408
37	22.2522476088093	26.318928510945355	29.791401285610146	21.63742259463519
38	21.923365779669254	26.517148453031353	30.087472572783362	21.47201319451603
39	22.538913514070714	26.32055605460409	29.506530990914044	21.63399944041115
40	22.471173811241847	26.60918609274449	29.92180482129972	20.997835274713946
41	21.916900443992844	26.7743146826887	30.195194863525582	21.113590009792876
42	22.44221749342837	27.591284947463755	29.247999057513752	20.718498501594127
43	21.699176036581324	27.062669818197882	29.30703130177384	21.93112284344695
44	21.58494289522323	27.61721022363276	28.760778480593213	22.037068400550798
45	21.787055445107136	27.960385833149253	28.196745453206685	22.055813268536927
46	21.473223818394683	27.669334589981666	28.013931124872066	22.84351046675159
47	22.338232262244865	27.71920007068595	27.672812417164906	22.26975524990428
48	22.455876830642133	28.57532047742116	26.655033023348278	22.313769668588428
49	22.42323834769163	28.41690597157794	26.62616891245122	22.533686768279214
50	22.251183612761665	29.352860182751282	26.100594199377085	22.29536200510997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	64.5
2	60.0
3	181.5
4	303.0
5	310.0
6	317.0
7	245.0
8	173.0
9	244.5
10	316.0
11	478.5
12	641.0
13	1125.0
14	1609.0
15	2084.0
16	2559.0
17	2476.0
18	2393.0
19	2278.5
20	2164.0
21	2139.5
22	2115.0
23	1856.0
24	1597.0
25	1446.0
26	1295.0
27	1553.0
28	1811.0
29	2110.5
30	2410.0
31	2905.0
32	3400.0
33	3977.0
34	4554.0
35	4888.5
36	5223.0
37	5545.0
38	5867.0
39	5928.0
40	5989.0
41	6281.5
42	6574.0
43	6686.0
44	6798.0
45	7220.5
46	7643.0
47	7977.5
48	8312.0
49	8541.0
50	8770.0
51	8453.5
52	8137.0
53	8135.5
54	8134.0
55	8036.0
56	7938.0
57	7673.5
58	7409.0
59	6732.0
60	6055.0
61	5335.5
62	4616.0
63	3925.0
64	3234.0
65	2793.5
66	2353.0
67	2003.0
68	1653.0
69	1457.0
70	1261.0
71	1029.5
72	798.0
73	646.5
74	495.0
75	372.5
76	250.0
77	219.0
78	188.0
79	165.5
80	143.0
81	110.0
82	77.0
83	65.0
84	53.0
85	45.5
86	38.0
87	24.0
88	10.0
89	7.0
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03534245364984464
2	0.005154107823935676
3	7.363011177050966E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.363011177050966E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	7.363011177050966E-4
25	0.0
26	7.363011177050966E-4
27	0.0
28	0.00220890335311529
29	0.0014726022354101932
30	0.0014726022354101932
31	0.0
32	0.0014726022354101932
33	7.363011177050966E-4
34	0.0014726022354101932
35	0.0
36	7.363011177050966E-4
37	0.00220890335311529
38	0.0
39	0.0
40	0.0
41	7.363011177050966E-4
42	0.00220890335311529
43	0.005154107823935676
44	0.008099312294756064
45	0.0029452044708203865
46	0.00220890335311529
47	0.0014726022354101932
48	7.363011177050966E-4
49	0.0029452044708203865
50	7.363011177050966E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	135814.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.03738937075707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.31186047683856	63.77030350332071
2	8.776652048994404	12.820475061481144
3	1.772266747315893	3.88325209477668
4	0.6099097736781088	1.7818487048463338
5	0.34074298099702605	1.2443488889216134
6	0.21775291093301075	0.9542462485458054
7	0.1421442613034931	0.7267292031749304
8	0.10282776349614395	0.6008217120473589
9	0.07863299561469832	0.5168833846289779
>10	0.5665608145571853	7.9491068667442235
>50	0.06250315036040123	3.1425331703653523
>100	0.01713796058269066	2.0837321631054238
>500	0.0010081153283935682	0.525718998041439
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	714	0.525718998041439	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.3320718040849986	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	334	0.2459245733135023	No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	198	0.14578762130560916	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	194	0.14284241683478874	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	178	0.1310615989515072	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	165	0.12148968442134095	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	137	0.10087325312559824	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	136	0.10013695200789316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	7.363011177050967E-4
13	0.0	0.0	0.0	0.0	7.363011177050967E-4
14	0.0	0.0	0.0	0.0	7.363011177050967E-4
15	0.0	0.0	0.0	0.0	7.363011177050967E-4
16	0.0	0.0	0.0	0.0	7.363011177050967E-4
17	0.0	0.0	0.0	0.0	7.363011177050967E-4
18	0.0	0.0	0.0	0.0014726022354101934	7.363011177050967E-4
19	0.0	0.0	0.0	0.0014726022354101934	7.363011177050967E-4
20	0.0	0.0	0.0	0.0014726022354101934	7.363011177050967E-4
21	0.0	0.0	0.0	0.0014726022354101934	0.0014726022354101934
22	0.0	0.0	0.0	0.002945204470820387	0.0014726022354101934
23	0.0	0.0	0.0	0.005890408941640774	0.0014726022354101934
24	0.0	0.0	0.0	0.00883561341246116	0.0014726022354101934
25	0.0	0.0	0.0	0.01619862458951213	0.0014726022354101934
26	0.0	0.0	0.0	0.016934925707217222	0.0014726022354101934
27	0.0	0.0	0.0	0.019880130178037612	0.0014726022354101934
28	0.0	0.0	0.0	0.027979442472793672	0.0014726022354101934
29	0.0	0.0	0.0	0.03755135700295993	0.0014726022354101934
30	0.0	0.0	0.0	0.0662671005934587	0.0014726022354101934
31	0.0	0.0	0.0	0.09645544641936767	0.0014726022354101934
32	0.0	0.0	0.0	0.13842461012855817	0.0014726022354101934
33	0.0	0.0	0.0	0.16714035371905694	0.0014726022354101934
34	0.0	0.0	0.0	0.19364719395644042	0.0014726022354101934
35	0.0	0.0	0.0	0.22678074425316977	0.0014726022354101934
36	0.0	0.0	0.0	0.27758552137482145	0.0014726022354101934
37	0.0	0.0	0.0	0.3467978264391005	0.0014726022354101934
38	0.0	0.0	0.0	0.4182190348564949	0.0014726022354101934
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	25	0.002345566	35.199707	41
AATAGAA	25	0.002345566	35.199707	36
CGCGGTC	30	0.005727539	29.333088	44
TCCGAAA	30	0.005727539	29.333088	21
TTCCGAA	30	0.005727539	29.333088	20
ACTGTTC	30	0.005727539	29.333088	8
AACTGCC	30	0.005727539	29.333088	7
ATACATG	135	0.0	27.713675	1
ATAGAAC	50	8.2885E-5	26.39978	37
TCCTGCA	45	0.0013919082	24.44424	2
TCAGTTC	45	0.0013919082	24.44424	16
AAATTTA	65	1.798182E-5	23.692108	44
TTAGGAC	85	2.3210669E-7	23.293922	3
GATTTCG	50	0.002568895	21.999817	41
ATTTCGT	50	0.002568895	21.999817	42
GACGTGA	80	3.5768317E-6	21.999815	7
GTCCTAC	105	7.590643E-8	20.959923	1
CTTTAGG	95	6.750997E-7	20.849607	1
ATAACGC	65	4.9015076E-4	20.307522	3
GGACGTG	120	1.4037141E-8	20.166498	6
>>END_MODULE