##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062239_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	202643
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.179576891380407	32.0	32.0	32.0	14.0	32.0
2	27.740721367133332	32.0	21.0	32.0	14.0	32.0
3	27.94742971629911	32.0	21.0	32.0	14.0	32.0
4	27.91157849025133	32.0	21.0	32.0	14.0	32.0
5	27.942800886287706	32.0	21.0	32.0	14.0	32.0
6	30.99807543315091	36.0	32.0	36.0	14.0	36.0
7	30.97219741121085	36.0	32.0	36.0	14.0	36.0
8	30.784571882571814	36.0	27.0	36.0	14.0	36.0
9	31.138371421662725	36.0	32.0	36.0	14.0	36.0
10	30.23851305004367	36.0	21.0	36.0	14.0	36.0
11	31.443277093213187	36.0	32.0	36.0	14.0	36.0
12	30.683699905745573	36.0	32.0	36.0	14.0	36.0
13	31.058235418938725	36.0	32.0	36.0	14.0	36.0
14	30.677595574483206	36.0	32.0	36.0	14.0	36.0
15	30.523867096322103	36.0	27.0	36.0	14.0	36.0
16	30.58179655847969	36.0	27.0	36.0	14.0	36.0
17	30.420858356814694	36.0	21.0	36.0	14.0	36.0
18	30.44781709706232	36.0	21.0	36.0	14.0	36.0
19	30.463618284372025	36.0	27.0	36.0	14.0	36.0
20	30.434848477371535	36.0	27.0	36.0	14.0	36.0
21	30.37972197411211	36.0	21.0	36.0	14.0	36.0
22	30.241982205158827	36.0	21.0	36.0	14.0	36.0
23	30.199148255799606	36.0	21.0	36.0	14.0	36.0
24	30.156057697527178	36.0	21.0	36.0	14.0	36.0
25	29.27780875727264	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	7.0
5	17.0
6	36.0
7	13.0
8	20.0
9	14.0
10	7.0
11	4.0
12	8.0
13	5.0
14	127.0
15	275.0
16	471.0
17	784.0
18	1288.0
19	2032.0
20	2859.0
21	3856.0
22	4813.0
23	5895.0
24	6820.0
25	7935.0
26	9215.0
27	10633.0
28	12072.0
29	13632.0
30	15416.0
31	17791.0
32	20891.0
33	22593.0
34	26547.0
35	16565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.15476554712251	20.680806545533503	13.027407941098094	27.13701996624589
2	16.170882640321356	19.583304546934986	38.769356796715385	25.476456016028266
3	18.03417833332511	22.931460746238457	29.37678577597055	29.65757514446588
4	12.466318584245487	15.907004747475744	35.440745383301916	36.18593128497685
5	14.65098945144555	35.74464304224851	33.72674455912769	15.87762294717825
6	33.42483337447544	35.440631942730185	16.705504813626266	14.429029869168106
7	29.11717495987159	30.780096308186195	20.670206198296086	19.43252253364613
8	27.20098375714236	33.27588165283053	19.282528927497296	20.240605662529816
9	25.530317622596595	14.722950714416244	19.623357188365855	40.12337447462131
10	16.223945470710262	26.582534821693176	31.132569396423985	26.060950311172576
11	35.569869739825435	21.70311349973573	22.52606932390178	20.200947436537064
12	23.371779989626813	23.906740102249994	29.09037022401146	23.631109684111735
13	28.294253918232066	20.11647262794454	25.61459316081422	25.974680293009172
14	22.448979591836736	19.559634115020646	26.274769346267064	31.716616946875554
15	24.180872451726675	27.496900331454597	22.792813708685493	25.529413508133235
16	24.21112938515259	26.69146009591402	24.29410341131904	24.803307107614348
17	22.610439386755175	26.90601785996523	26.036233602023074	24.44730915125652
18	23.18831274663295	25.509564049250038	26.957135872222526	24.344987331894487
19	23.972186692873336	25.60681109964295	26.634994789943356	23.786007417540358
20	24.49371620453816	24.780128885706524	26.08923236463297	24.636922545122342
21	24.795065776478488	24.660747442025837	26.25380239402679	24.29038438746889
22	24.27376260605881	24.935549826651258	26.34999654290258	24.440691024387355
23	23.260200781199845	24.84679693248201	26.78992044798009	25.10308183833805
24	23.490980202371368	25.225803584215228	26.648032829791752	24.635183383621648
25	23.583992573059565	24.66593582412373	26.80365026221445	24.946421340602253
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	18.0
2	48.0
3	78.0
4	78.0
5	78.0
6	202.0
7	326.0
8	326.0
9	326.0
10	403.0
11	480.0
12	480.0
13	480.0
14	678.0
15	876.0
16	876.0
17	876.0
18	1508.5
19	2141.0
20	2141.0
21	2141.0
22	3123.5
23	4106.0
24	4106.0
25	4106.0
26	5824.5
27	7543.0
28	7543.0
29	7543.0
30	8941.0
31	10339.0
32	10339.0
33	10339.0
34	12268.0
35	14197.0
36	14197.0
37	14197.0
38	15889.5
39	17582.0
40	17582.0
41	17582.0
42	19465.0
43	21348.0
44	21348.0
45	21348.0
46	23124.0
47	24900.0
48	24900.0
49	24900.0
50	25270.5
51	25641.0
52	25641.0
53	25641.0
54	23962.5
55	22284.0
56	22284.0
57	22284.0
58	20357.5
59	18431.0
60	18431.0
61	18431.0
62	15863.0
63	13295.0
64	13295.0
65	13295.0
66	11119.5
67	8944.0
68	8944.0
69	8944.0
70	6957.5
71	4971.0
72	4971.0
73	4971.0
74	3729.5
75	2488.0
76	2488.0
77	2488.0
78	1987.0
79	1486.0
80	1486.0
81	1486.0
82	1041.5
83	597.0
84	597.0
85	597.0
86	440.5
87	284.0
88	284.0
89	284.0
90	185.5
91	87.0
92	87.0
93	87.0
94	60.5
95	34.0
96	34.0
97	34.0
98	100.5
99	167.0
100	167.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.934786792536629E-4
2	4.934786792536629E-4
3	0.0
4	0.004441308113282966
5	0.027634806038205117
6	0.04589351717059064
7	0.08290441811461535
8	0.07599571660506409
9	0.08389137547312268
10	0.09030659830342029
11	0.09968269320923988
12	0.09770877849222524
13	0.09474790641670326
14	0.086358768869391
15	0.09968269320923988
16	0.08389137547312268
17	0.08833268358640566
18	0.08191746075610803
19	0.07402180188804942
20	0.06810005773700548
21	0.06859353641625913
22	0.07945006735983971
23	0.06612614301999083
24	0.07106092981252746
25	0.0676065790577518
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	202643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.46678184927502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.72356179813231	54.983689358314805
2	11.104846038199701	14.76206758181611
3	2.688026833614501	5.359934794645574
4	1.1299930946124244	3.0042801804316484
5	0.6029348255620188	2.0037568759980697
6	0.39037309437458917	1.556810598217336
7	0.25786941895974336	1.1997825290917596
8	0.17804290910298098	0.9467151359326511
9	0.12686530738241888	0.7589095839025609
>10	0.6912871142389825	8.56792206610801
>50	0.0806330036783735	3.6195667411543093
>100	0.02359990351562151	2.3025882320488678
>500	0.0019666586263017926	0.9339763223383004
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	785	0.38738076321412535	No Hit
TATCAACGCAGAGTACTTTTTTTTT	644	0.31780026943935885	No Hit
GGTATCAACGCAGAGTACTTTTTTT	394	0.19443059962594317	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	258	0.12731749924744504	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	228	0.11251313886983512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	120	0.0	15.03619	1
AGGCCCG	40	0.00524497	14.255378	10
ATTTGCC	40	0.005262215	14.248332	16
TGCCAAG	60	2.5553152E-5	14.244811	19
TTGCCAA	60	4.0772694E-4	12.662055	18
AAAGCAT	55	0.003043989	12.095473	11
CGTCAAT	55	0.0030609563	12.086507	19
CACACAG	80	2.8338169E-5	11.876545	5
ATGTGGT	60	0.005829359	11.087517	7
GCAGGCC	60	0.005829359	11.087517	8
CCAGCCC	60	0.0058508706	11.082036	3
CGAGCCG	60	0.0058508706	11.082036	15
CAAAGCA	60	0.0058508706	11.082036	4
CCGTCAA	60	0.0058616516	11.079298	18
GTATCAA	275	0.0	11.050519	1
TCAAAGC	70	0.0014831607	10.855871	3
AGCCGCC	70	0.0014862244	10.8531885	17
GTTCAAA	80	3.7528336E-4	10.683609	1
TTCAAAG	85	6.562249E-4	10.0551605	2
CTGTGTG	95	1.621641E-4	10.003774	9
>>END_MODULE