FastQCFastQC Report
Thu 2 Feb 2017
SRR4062239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202643
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC2680.13225228603998165No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG2270.11201966019058146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC502.3486664E-630.84
GGTATCA1250.029.921
CGACCCG300.005733877429.3333325
GTATATG300.005733877429.3333321
TCGTTTA508.307834E-526.430
CGTTTAT508.307834E-526.431
ATAACGC609.7007905E-625.6666663
TAAACAC450.00139420524.4444455
TCTACAC450.00139420524.4444453
CAGGATA450.00139420524.4444454
GGGTTAA450.00139420524.4444451
GGTCGGA551.587244E-424.038
AACGCCG651.803746E-523.6923075
CGGGTCA602.8613643E-422.042
ACCATAC500.002573122.01
CGGCATC500.002573122.025
GTCTACA602.8613643E-422.02
TCGGCAT602.8613643E-422.024
GGCGGGT904.0472332E-722.040
ACTCCCC602.8613643E-422.06
Produced by FastQC (version 0.11.5)