##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062239_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	202643
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.058536440933068	32.0	32.0	32.0	21.0	32.0
2	28.584880800225026	32.0	32.0	32.0	14.0	32.0
3	30.35375512600978	32.0	32.0	32.0	21.0	32.0
4	30.93304481279886	32.0	32.0	32.0	32.0	32.0
5	30.624635442625703	32.0	32.0	32.0	32.0	32.0
6	34.18800057243527	36.0	36.0	36.0	32.0	36.0
7	34.214934638748936	36.0	36.0	36.0	32.0	36.0
8	34.03656183534591	36.0	36.0	36.0	32.0	36.0
9	34.40952808633903	36.0	36.0	36.0	32.0	36.0
10	33.90236030852287	36.0	36.0	36.0	32.0	36.0
11	34.577577315772075	36.0	36.0	36.0	32.0	36.0
12	34.23469845985304	36.0	36.0	36.0	32.0	36.0
13	34.436728631139495	36.0	36.0	36.0	32.0	36.0
14	34.302206343174944	36.0	36.0	36.0	32.0	36.0
15	34.18387509067671	36.0	36.0	36.0	32.0	36.0
16	34.25077599522313	36.0	36.0	36.0	32.0	36.0
17	34.18158534960497	36.0	36.0	36.0	32.0	36.0
18	34.19938019077886	36.0	36.0	36.0	32.0	36.0
19	34.21695296654708	36.0	36.0	36.0	32.0	36.0
20	34.24185390070222	36.0	36.0	36.0	32.0	36.0
21	34.22416762483777	36.0	36.0	36.0	32.0	36.0
22	34.18650533203713	36.0	36.0	36.0	32.0	36.0
23	34.108950222805625	36.0	36.0	36.0	32.0	36.0
24	34.067275948342655	36.0	36.0	36.0	32.0	36.0
25	34.03770177109498	36.0	36.0	36.0	32.0	36.0
26	33.954150895910544	36.0	36.0	36.0	32.0	36.0
27	33.89183934308118	36.0	36.0	36.0	32.0	36.0
28	33.87510548106769	36.0	36.0	36.0	32.0	36.0
29	33.806151705215576	36.0	36.0	36.0	32.0	36.0
30	33.768252542648895	36.0	36.0	36.0	32.0	36.0
31	33.79772802416072	36.0	36.0	36.0	32.0	36.0
32	33.74739813366364	36.0	36.0	36.0	32.0	36.0
33	33.67896251042474	36.0	36.0	36.0	27.0	36.0
34	33.63924241153161	36.0	36.0	36.0	27.0	36.0
35	33.656573382747	36.0	36.0	36.0	27.0	36.0
36	33.5686946995455	36.0	36.0	36.0	27.0	36.0
37	33.60451631687253	36.0	36.0	36.0	27.0	36.0
38	33.53495062745814	36.0	36.0	36.0	27.0	36.0
39	33.50690623411616	36.0	36.0	36.0	21.0	36.0
40	33.481541430002515	36.0	36.0	36.0	21.0	36.0
41	33.41028804350508	36.0	36.0	36.0	21.0	36.0
42	33.358255651564576	36.0	36.0	36.0	21.0	36.0
43	33.373341294789356	36.0	36.0	36.0	21.0	36.0
44	33.28454967603125	36.0	36.0	36.0	21.0	36.0
45	33.24555499079662	36.0	36.0	36.0	21.0	36.0
46	33.231421761422794	36.0	36.0	36.0	21.0	36.0
47	33.20004638699585	36.0	36.0	36.0	21.0	36.0
48	33.12980956657768	36.0	36.0	36.0	21.0	36.0
49	33.06421144574448	36.0	36.0	36.0	14.0	36.0
50	32.440395177726344	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	5.0
21	6.0
22	31.0
23	83.0
24	184.0
25	491.0
26	1036.0
27	1937.0
28	3563.0
29	5984.0
30	9975.0
31	15995.0
32	25042.0
33	38936.0
34	55651.0
35	43721.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6852557395819	19.218469313224233	13.823854551197268	25.272420395996605
2	18.267147658127588	21.12299333290564	35.61442382190814	24.995435187058632
3	21.55761610319626	23.213237072092298	28.915876689547627	26.31327013516381
4	16.12096149385866	17.207601545575223	35.61830411117088	31.053132849395244
5	20.113697487700044	32.67371683206427	30.636143365425895	16.57644231480979
6	34.87727124682938	36.012771291242686	15.108417800850763	14.00153966107717
7	29.857434009563615	30.635156408067388	19.30735332579956	20.200056256569436
8	28.003928090286863	33.39172831037835	17.619656242752033	20.98468735658276
9	26.094165601575188	14.127801108353113	17.962130446154074	41.81590284391763
10	16.607037992923516	26.25503965101188	30.209777786550735	26.928144569513872
11	37.725458071584015	20.67774361808698	21.150002714132736	20.446795596196267
12	23.99046599191682	23.192510967563646	27.915595406700454	24.901427633819083
13	29.3836944774801	19.126246650513465	24.512566434567194	26.97749243743924
14	23.458989454360623	18.768474608054557	24.407948954565416	33.3645869830194
15	25.299171449297532	26.323633187428136	21.694309697349524	26.682885665924804
16	25.55775427722645	26.140059118745775	23.08789348756187	25.214293116465903
17	24.005270352294428	26.331528846296198	24.401040253055868	25.262160548353506
18	24.859975424761775	25.28239317420291	25.3218714685432	24.535759932492116
19	25.37763455929887	25.611049974585846	24.89599936834729	24.115316097767998
20	25.86963280251477	24.43064897381109	24.473581618906152	25.22613660476799
21	26.00731335402654	24.50022946758585	24.49282728739705	24.999629890990562
22	25.48422595401766	24.641857848531654	24.654194815512994	25.219721381937692
23	24.50022946758585	24.372911968338407	25.42698242722423	25.699876136851508
24	24.445080486769772	25.105851699055474	25.145823669328177	25.303244144846577
25	24.75979925287328	24.287540156827525	25.438332436847066	25.51432815345213
26	24.62470761243967	25.035777380799633	25.208002289752372	25.13151271700832
27	24.8362884481576	24.949295065706686	25.16395829118203	25.05045819495369
28	24.71242529966493	24.819510173062973	25.46843463628154	24.999629890990562
29	24.839000991901937	25.14397382563252	25.123741000093762	24.89328418237178
30	24.510340947784506	25.35173040006711	25.521488741172817	24.61643991097557
31	24.995682061556533	24.97544943570713	24.959658117971014	25.069210384765327
32	25.301270220388666	25.150264999358473	24.496896003789935	25.051568776462922
33	24.60928825570091	25.053912545708464	25.18123004495591	25.155569153634715
34	24.873792827780875	24.859975424761775	25.463499849489	24.802731897968346
35	25.74342929896073	24.69576889292447	24.96323565697141	24.597566151143397
36	24.304811910601405	25.4358650434508	24.978410307782653	25.280912738165146
37	25.443886262472738	24.845787151725705	25.081177643331586	24.62914894246997
38	24.838755841553866	24.75437098740149	25.215773552503663	25.19109961854098
39	25.172595871516624	25.2446444697766	24.815313781515094	24.767445877191683
40	25.435497083526613	25.449314554731988	24.922276724469754	24.192911637271642
41	24.65826432822416	25.15964114053355	25.449808035846466	24.732286495395822
42	25.5044784958176	25.714215214548325	24.717348927875243	24.063957361758828
43	24.841713589190633	24.906360572249174	25.27400944536836	24.977916393191833
44	24.965949467035138	25.46535728385314	24.803099091985786	24.765594157125935
45	24.88242754427781	25.85903148918531	24.75264137069991	24.505899595836972
46	24.501579155151994	25.419463087248324	25.004934859849982	25.074022897749703
47	24.81506521449474	25.21627130019394	25.450678299833697	24.517985185477624
48	25.61847657117477	25.61206109507242	24.352160288202924	24.417302045549878
49	24.74277169970539	25.668053355441394	24.75560227199826	24.83357267285495
50	25.36394232192734	25.30225718261762	24.530452719574424	24.803347775880617
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	4.0
4	8.0
5	9.5
6	11.0
7	13.0
8	15.0
9	20.0
10	25.0
11	48.5
12	72.0
13	121.0
14	170.0
15	227.0
16	284.0
17	361.0
18	438.0
19	491.5
20	545.0
21	708.0
22	871.0
23	1109.5
24	1348.0
25	1664.0
26	1980.0
27	2276.0
28	2572.0
29	2927.0
30	3282.0
31	3575.0
32	3868.0
33	4428.0
34	4988.0
35	5535.0
36	6082.0
37	6660.5
38	7239.0
39	7972.5
40	8706.0
41	9360.0
42	10014.0
43	10272.5
44	10531.0
45	11167.5
46	11804.0
47	12357.0
48	12910.0
49	13377.0
50	13844.0
51	13690.0
52	13536.0
53	13579.5
54	13623.0
55	13875.5
56	14128.0
57	13689.0
58	13250.0
59	12214.0
60	11178.0
61	10209.0
62	9240.0
63	8140.5
64	7041.0
65	6149.0
66	5257.0
67	4623.5
68	3990.0
69	3627.0
70	3264.0
71	2603.0
72	1942.0
73	1714.0
74	1486.0
75	1229.0
76	972.0
77	837.0
78	702.0
79	613.5
80	525.0
81	439.0
82	353.0
83	300.0
84	247.0
85	178.0
86	109.0
87	84.0
88	59.0
89	48.5
90	38.0
91	32.0
92	26.0
93	21.0
94	16.0
95	15.0
96	14.0
97	13.0
98	12.0
99	19.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0074021801888049426
2	0.002960872075521977
3	0.0
4	0.0
5	0.0
6	4.934786792536629E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	4.934786792536629E-4
25	0.0
26	4.934786792536629E-4
27	0.0
28	0.0
29	9.869573585073257E-4
30	9.869573585073257E-4
31	0.0
32	4.934786792536629E-4
33	0.0
34	0.0
35	4.934786792536629E-4
36	0.0
37	4.934786792536629E-4
38	0.0
39	9.869573585073257E-4
40	4.934786792536629E-4
41	4.934786792536629E-4
42	0.003947829434029303
43	0.0019739147170146514
44	0.0014804360377609885
45	9.869573585073257E-4
46	0.0014804360377609885
47	0.0019739147170146514
48	0.003947829434029303
49	9.869573585073257E-4
50	4.934786792536629E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	202643.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.34846897656328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.25816136247877	60.1169081199308
2	10.387455598143823	14.822581069791863
3	2.4361111067992374	5.214383931807799
4	1.0117331399887637	2.8874244220419705
5	0.516791002826321	1.8436123416260393
6	0.33694744317637926	1.4424410517721329
7	0.22401422584137248	1.1188150429926405
8	0.10599304189346252	0.6049953009013826
9	0.10496357449974808	0.6740091304978019
>10	0.5589900061939627	7.4062729662551545
>50	0.04216830701334483	2.1039518472606136
>100	0.016671191144810745	1.7646047751218048
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	268	0.13225228603998165	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	227	0.11201966019058146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	9.869573585073257E-4	0.0
35	0.0	0.0	0.0	0.0014804360377609885	0.0
36	0.0	0.0	0.0	0.0014804360377609885	0.0
37	0.0	0.0	0.0	0.0014804360377609885	0.0
38	0.0	0.0	0.0	0.004441308113282965	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	50	2.3486664E-6	30.8	4
GGTATCA	125	0.0	29.92	1
CGACCCG	30	0.0057338774	29.333332	5
GTATATG	30	0.0057338774	29.333332	1
TCGTTTA	50	8.307834E-5	26.4	30
CGTTTAT	50	8.307834E-5	26.4	31
ATAACGC	60	9.7007905E-6	25.666666	3
TAAACAC	45	0.001394205	24.444445	5
TCTACAC	45	0.001394205	24.444445	3
CAGGATA	45	0.001394205	24.444445	4
GGGTTAA	45	0.001394205	24.444445	1
GGTCGGA	55	1.587244E-4	24.0	38
AACGCCG	65	1.803746E-5	23.692307	5
CGGGTCA	60	2.8613643E-4	22.0	42
ACCATAC	50	0.0025731	22.0	1
CGGCATC	50	0.0025731	22.0	25
GTCTACA	60	2.8613643E-4	22.0	2
TCGGCAT	60	2.8613643E-4	22.0	24
GGCGGGT	90	4.0472332E-7	22.0	40
ACTCCCC	60	2.8613643E-4	22.0	6
>>END_MODULE