FastQCFastQC Report
Thu 2 Feb 2017
SRR4062238_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062238_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614906
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43380.16589506467918924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA3900.014.13088310
GGTATCA8500.014.07531451
GTATCAA25050.013.3426291
CCGTCGT4150.013.2793719
GTCGTAG4400.012.52534111
ACCGTCG4650.012.2601548
CGCGTAA1800.012.14119110
TCGAACG907.4835825E-611.6086433
CGACCAT3550.011.50922410
CGCATCG3250.011.40150513
GCGTAAC1850.011.29965611
CGCAAGA4050.011.2553562
GTCCTAC4000.011.1569211
AGCGACG600.005870021411.0852228
CGAGCCG3950.011.064116515
AAGACGG4300.011.0469125
TACCGTC5000.011.0218787
CTTATAC1855.456968E-1210.7814893
CCTATTC3700.010.7814893
CAAGACG4500.010.7654084
Produced by FastQC (version 0.11.5)