##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062238_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2614906
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.168625946783557	32.0	32.0	32.0	32.0	32.0
2	30.58773623220108	32.0	32.0	32.0	32.0	32.0
3	30.629151105240496	32.0	32.0	32.0	32.0	32.0
4	30.640246723974016	32.0	32.0	32.0	32.0	32.0
5	30.515470536990623	32.0	32.0	32.0	32.0	32.0
6	34.175759664018514	36.0	36.0	36.0	32.0	36.0
7	34.09871635921138	36.0	36.0	36.0	32.0	36.0
8	34.03814898126357	36.0	36.0	36.0	32.0	36.0
9	34.223935009518506	36.0	36.0	36.0	32.0	36.0
10	33.81229229654909	36.0	36.0	36.0	32.0	36.0
11	34.216029180398834	36.0	36.0	36.0	32.0	36.0
12	33.983435733445106	36.0	36.0	36.0	32.0	36.0
13	34.100117556807014	36.0	36.0	36.0	32.0	36.0
14	33.98508665321048	36.0	36.0	36.0	32.0	36.0
15	33.913251183790166	36.0	36.0	36.0	32.0	36.0
16	33.92586196215084	36.0	36.0	36.0	32.0	36.0
17	33.848829365185594	36.0	36.0	36.0	32.0	36.0
18	33.85932152054414	36.0	36.0	36.0	32.0	36.0
19	33.870439702230215	36.0	36.0	36.0	32.0	36.0
20	33.85361538808661	36.0	36.0	36.0	32.0	36.0
21	33.83828864211562	36.0	36.0	36.0	32.0	36.0
22	33.804235792797144	36.0	36.0	36.0	32.0	36.0
23	33.759807044689175	36.0	36.0	36.0	32.0	36.0
24	33.73873668881405	36.0	36.0	36.0	32.0	36.0
25	33.35280847571576	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	48.0
5	200.0
6	558.0
7	130.0
8	455.0
9	343.0
10	194.0
11	50.0
12	117.0
13	119.0
14	368.0
15	564.0
16	869.0
17	1269.0
18	1828.0
19	2501.0
20	3954.0
21	5753.0
22	9031.0
23	13245.0
24	18929.0
25	26477.0
26	36314.0
27	46165.0
28	60897.0
29	79953.0
30	103906.0
31	138567.0
32	194651.0
33	269743.0
34	553792.0
35	1043915.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.41479739597894	17.2062263684094	11.719165590453708	25.65981064515796
2	16.659489846648054	19.151439825614748	37.64170713985238	26.547363187884816
3	19.28155240300923	22.892223299812457	28.019708683507666	29.80651561367064
4	12.689080001254476	15.21126857525757	35.67803371332278	36.42161771016517
5	14.8563003700418	36.46084263028451	33.54821235724623	15.134644642427455
6	35.03439640686987	35.02911554337981	16.398573554583656	13.53791449516667
7	30.83618004188997	30.077474626552547	20.08498205070976	19.001363280847723
8	28.119894993106865	33.45075208105301	18.916324881250866	19.513028044589262
9	26.854634854584315	14.629440598157405	18.35735678015617	40.15856776710211
10	15.623325852041987	27.102940450018632	31.6198610478735	25.653872650065885
11	37.24260663665013	21.55281788982727	21.85256203309785	19.352013440424752
12	24.26923213896623	23.60751735565293	28.838970099146167	23.28428040623467
13	29.13858621566399	19.74138670457017	25.301277711575988	25.818749368189852
14	23.247473637540786	19.520701355127272	25.33545085529152	31.896374152040423
15	24.97666957570986	27.46300160412224	22.113297978055424	25.44703084211248
16	25.520049621521036	26.391527584741382	23.51447485804646	24.57394793569112
17	23.966018443498598	26.368137868291797	25.08404641966081	24.581797268548797
18	24.508799495246496	25.852154390865433	25.707014787317455	23.932031326570616
19	25.275882080029156	25.33678514501131	25.359638149281793	24.027694625677743
20	25.31937062150093	25.029378192844966	25.062297079941604	24.5889541057125
21	26.09705515113145	24.707310332578754	24.830487648966848	24.36514686732295
22	25.462087077027913	24.667804928469433	25.20008268987087	24.670025304631782
23	24.35688204719949	24.767983036965024	25.517158232353776	25.35797668348171
24	24.437841193821438	25.183060939675823	25.428724318837233	24.950373547665507
25	24.645332520856712	24.929380369197133	25.43611928095819	24.989167828987963
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	217.0
1	217.0
2	482.0
3	747.0
4	747.0
5	747.0
6	1743.5
7	2740.0
8	2740.0
9	2740.0
10	3481.5
11	4223.0
12	4223.0
13	4223.0
14	5930.0
15	7637.0
16	7637.0
17	7637.0
18	12725.0
19	17813.0
20	17813.0
21	17813.0
22	27807.5
23	37802.0
24	37802.0
25	37802.0
26	55055.5
27	72309.0
28	72309.0
29	72309.0
30	91916.5
31	111524.0
32	111524.0
33	111524.0
34	137616.0
35	163708.0
36	163708.0
37	163708.0
38	190847.5
39	217987.0
40	217987.0
41	217987.0
42	249493.5
43	281000.0
44	281000.0
45	281000.0
46	310128.0
47	339256.0
48	339256.0
49	339256.0
50	347204.0
51	355152.0
52	355152.0
53	355152.0
54	333752.5
55	312353.0
56	312353.0
57	312353.0
58	285006.0
59	257659.0
60	257659.0
61	257659.0
62	223741.0
63	189823.0
64	189823.0
65	189823.0
66	154396.5
67	118970.0
68	118970.0
69	118970.0
70	90310.0
71	61650.0
72	61650.0
73	61650.0
74	46508.0
75	31366.0
76	31366.0
77	31366.0
78	24724.5
79	18083.0
80	18083.0
81	18083.0
82	12548.5
83	7014.0
84	7014.0
85	7014.0
86	5151.0
87	3288.0
88	3288.0
89	3288.0
90	2219.5
91	1151.0
92	1151.0
93	1151.0
94	734.0
95	317.0
96	317.0
97	317.0
98	717.0
99	1117.0
100	1117.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010325418963435015
2	2.2945375474300032E-4
3	6.883612642290011E-4
4	0.010401903548349349
5	0.033538490484935214
6	0.06489717029981193
7	0.10803447619149599
8	0.11040549832384032
9	0.12053970582498952
10	0.133962750477455
11	0.14069339394991637
12	0.13419220423219802
13	0.12857058724099452
14	0.11656250740944417
15	0.13419220423219802
16	0.11992782914567483
17	0.12444041965562051
18	0.11235585523915582
19	0.09782378410543248
20	0.09269931691617213
21	0.09224040940668614
22	0.10463091216280816
23	0.08283280546222312
24	0.09132259438771413
25	0.08864563391571245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2614906.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.56913916759565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.61458010938487	41.04177104146544
2	13.560401946816796	14.528381181151278
3	4.235273559994083	6.80639876244484
4	1.8393747088770978	3.94134879044772
5	0.9372719849519446	2.510442669988967
6	0.571322442113769	1.8363150846697862
7	0.38615316606894895	1.4480124885208638
8	0.28344377458579806	1.214707120558018
9	0.21237105982577428	1.0238881373079014
>10	1.20712882118033	11.90668309537111
>50	0.08837684093689091	3.269383968233376
>100	0.05476896478720846	5.860499698726843
>500	0.006809014626138466	2.451908411284407
>1k	0.002723605850445445	2.1602595498295885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4338	0.16589506467918924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.824229245716672E-5
2	0.0	0.0	0.0	0.0	3.824229245716672E-5
3	0.0	0.0	0.0	0.0	3.824229245716672E-5
4	0.0	0.0	0.0	0.0	3.824229245716672E-5
5	0.0	3.824229245716672E-5	0.0	0.0	3.824229245716672E-5
6	0.0	3.824229245716672E-5	0.0	0.0	7.648458491433343E-5
7	0.0	3.824229245716672E-5	0.0	0.0	7.648458491433343E-5
8	0.0	3.824229245716672E-5	0.0	0.0	7.648458491433343E-5
9	0.0	3.824229245716672E-5	0.0	0.0	7.648458491433343E-5
10	0.0	3.824229245716672E-5	0.0	0.0	7.648458491433343E-5
11	0.0	3.824229245716672E-5	0.0	0.0	7.648458491433343E-5
12	0.0	3.824229245716672E-5	0.0	0.0	7.648458491433343E-5
13	0.0	3.824229245716672E-5	0.0	3.824229245716672E-5	7.648458491433343E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	390	0.0	14.130883	10
GGTATCA	850	0.0	14.0753145	1
GTATCAA	2505	0.0	13.342629	1
CCGTCGT	415	0.0	13.279371	9
GTCGTAG	440	0.0	12.525341	11
ACCGTCG	465	0.0	12.260154	8
CGCGTAA	180	0.0	12.141191	10
TCGAACG	90	7.4835825E-6	11.608643	3
CGACCAT	355	0.0	11.509224	10
CGCATCG	325	0.0	11.401505	13
GCGTAAC	185	0.0	11.299656	11
CGCAAGA	405	0.0	11.255356	2
GTCCTAC	400	0.0	11.156921	1
AGCGACG	60	0.0058700214	11.085222	8
CGAGCCG	395	0.0	11.0641165	15
AAGACGG	430	0.0	11.046912	5
TACCGTC	500	0.0	11.021878	7
CTTATAC	185	5.456968E-12	10.781489	3
CCTATTC	370	0.0	10.781489	3
CAAGACG	450	0.0	10.765408	4
>>END_MODULE