FastQCFastQC Report
Thu 2 Feb 2017
SRR4062238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614906
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30050.028.4867921
GGTATCA13450.022.5785061
CGCAATA5150.021.78679536
TACGAAT5850.019.557441
CTAGCGG6050.018.90834429
ATACGAA5950.018.8585640
TCAACGC44450.018.8069044
AGTATCG951.598813E-518.5255838
ATCAACG45250.018.4747623
TCTAGCG6200.018.45123528
CAACGCA45400.018.413375
ACCGTCG5400.018.332618
AACGCAG45900.018.3086436
TATCAAC46600.018.1294252
GCGCAAT6300.017.8091635
CGCCGGT6550.017.8008237
TAGCGGC6450.017.73573330
CGAATGC6450.017.39665843
CGTCGTA5200.017.345810
GATATAC4700.017.3237631
Produced by FastQC (version 0.11.5)