##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062238_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2614906
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272892027476324	32.0	32.0	32.0	32.0	32.0
2	31.414177412113474	32.0	32.0	32.0	32.0	32.0
3	31.554360653882014	32.0	32.0	32.0	32.0	32.0
4	31.63763401055334	32.0	32.0	32.0	32.0	32.0
5	31.609480034846378	32.0	32.0	32.0	32.0	32.0
6	35.25057803225049	36.0	36.0	36.0	36.0	36.0
7	35.25381983138208	36.0	36.0	36.0	36.0	36.0
8	35.2023357627387	36.0	36.0	36.0	36.0	36.0
9	35.294414789671215	36.0	36.0	36.0	36.0	36.0
10	35.170012994730975	36.0	36.0	36.0	36.0	36.0
11	35.29907881965929	36.0	36.0	36.0	36.0	36.0
12	35.213915911317656	36.0	36.0	36.0	36.0	36.0
13	35.25584246623014	36.0	36.0	36.0	36.0	36.0
14	35.21991077308324	36.0	36.0	36.0	36.0	36.0
15	35.19511523549986	36.0	36.0	36.0	36.0	36.0
16	35.209876760388326	36.0	36.0	36.0	36.0	36.0
17	35.18736887673974	36.0	36.0	36.0	36.0	36.0
18	35.18575466957512	36.0	36.0	36.0	36.0	36.0
19	35.179144489323896	36.0	36.0	36.0	36.0	36.0
20	35.17929286941863	36.0	36.0	36.0	36.0	36.0
21	35.17135835857962	36.0	36.0	36.0	36.0	36.0
22	35.15445794227402	36.0	36.0	36.0	36.0	36.0
23	35.120884268880026	36.0	36.0	36.0	36.0	36.0
24	35.107502143480495	36.0	36.0	36.0	36.0	36.0
25	35.092701229030794	36.0	36.0	36.0	36.0	36.0
26	35.05375948504459	36.0	36.0	36.0	36.0	36.0
27	35.03894212640913	36.0	36.0	36.0	36.0	36.0
28	35.02146616360206	36.0	36.0	36.0	36.0	36.0
29	35.002892264578534	36.0	36.0	36.0	36.0	36.0
30	34.97854645635445	36.0	36.0	36.0	36.0	36.0
31	34.98511801189029	36.0	36.0	36.0	36.0	36.0
32	34.96074734617611	36.0	36.0	36.0	36.0	36.0
33	34.940852940794045	36.0	36.0	36.0	36.0	36.0
34	34.92879246902183	36.0	36.0	36.0	36.0	36.0
35	34.91226720960524	36.0	36.0	36.0	36.0	36.0
36	34.88946065365256	36.0	36.0	36.0	32.0	36.0
37	34.87825489711676	36.0	36.0	36.0	32.0	36.0
38	34.86382722744145	36.0	36.0	36.0	32.0	36.0
39	34.8486224743834	36.0	36.0	36.0	32.0	36.0
40	34.83566675054476	36.0	36.0	36.0	32.0	36.0
41	34.808395024524785	36.0	36.0	36.0	32.0	36.0
42	34.78585578219638	36.0	36.0	36.0	32.0	36.0
43	34.77825627383929	36.0	36.0	36.0	32.0	36.0
44	34.7359017876742	36.0	36.0	36.0	32.0	36.0
45	34.72206955049245	36.0	36.0	36.0	32.0	36.0
46	34.70355225006176	36.0	36.0	36.0	32.0	36.0
47	34.68020418324789	36.0	36.0	36.0	32.0	36.0
48	34.660457393114704	36.0	36.0	36.0	32.0	36.0
49	34.64002109444852	36.0	36.0	36.0	32.0	36.0
50	34.22813363080738	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	7.0
20	21.0
21	54.0
22	167.0
23	450.0
24	1146.0
25	2737.0
26	5868.0
27	10914.0
28	19556.0
29	31405.0
30	48017.0
31	71917.0
32	110497.0
33	190529.0
34	435974.0
35	1685645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.148789055730866	17.671779801745675	12.12164566467377	26.05778547784969
2	16.065058920398855	19.692297572394665	37.32902605515759	26.913617452048893
3	18.63700332708708	23.347164327507745	28.40624880492562	29.609583540479562
4	12.471165289433188	15.600457990044758	35.851144057296175	36.07723266322588
5	14.52671721277935	36.788549951699984	33.40192725857067	15.282805576949993
6	35.122195784010266	35.28667613547276	16.228065143550314	13.363062936966664
7	30.909562332259743	30.14226897639915	20.058197120661315	18.889971570679787
8	28.19458902155565	33.57906555723227	18.762777705967252	19.46356771524483
9	27.043801956934587	14.390612893924292	18.247692268861673	40.317892880279445
10	15.502754786697635	27.217185494069952	31.660103644379927	25.61995607485249
11	37.41996844246027	21.389602532557575	21.92254712024065	19.26788190474151
12	24.426728914920844	23.520348341393536	28.859813698848065	23.19310904483756
13	29.295163956180453	19.60517892421372	25.330738466315804	25.768918653290022
14	23.27705087678104	19.327195700342575	25.391237772983043	32.00451564989334
15	25.009847390307723	27.40997190721196	22.155060258380225	25.42512044410009
16	25.598621429715973	26.294254848113663	23.581628700022144	24.52549502214822
17	24.04587392433992	26.277885323602458	25.116160963338647	24.560079788718983
18	24.626075995935604	25.66164022145372	25.868645988015615	23.843637794595057
19	25.38607506350133	25.27402514660183	25.378961997104295	23.960937792792553
20	25.47710219239658	24.868895610197097	25.15116251055011	24.502839686856216
21	26.192309089622757	24.654891860316148	24.82384637300399	24.3289526770571
22	25.49127961005099	24.582986921900826	25.308099029181164	24.61763443886702
23	24.410303399283492	24.603312396936943	25.616810406806522	25.369573796973043
24	24.536718170682597	25.10416359928211	25.465786727253324	24.893331502781965
25	24.762639015885508	24.714912507136987	25.56878869891342	24.953659778064086
26	24.477732375487353	25.39516883087084	25.702599913189633	24.424498880452177
27	24.94216780571901	25.15449006900091	25.219311076973323	24.684031048306757
28	24.652725060892386	24.999015243269334	25.60417215612773	24.744087539710556
29	24.524837699090135	25.19572480129229	25.553023906113282	24.726413593504294
30	24.636530548949107	25.268684588023337	25.610001877723125	24.484782985304427
31	25.025593761891013	24.96463529128321	25.09603636092463	24.913734585901153
32	24.78002848309906	25.09067323827749	25.04344360973565	25.085854668887798
33	24.49797276987477	24.89619097953343	25.485812448516153	25.120023802075647
34	24.971891721557483	24.952808686261132	25.622129896194405	24.45316969598698
35	25.36860803738568	24.860750199338025	25.47102093575101	24.299620827525285
36	24.461623771463536	25.349535355080498	25.30253546980764	24.886305403648322
37	25.316948013680268	25.014331545730347	25.159310571384104	24.509409869205285
38	24.730257990153376	24.904910539805254	25.467837084774747	24.89699438526662
39	25.1299293811346	24.98066834373406	25.031760515388456	24.857641759742886
40	25.37351275610883	25.04910314107134	25.231519015612047	24.34586508720779
41	24.517214385227298	25.333046258007546	25.600324604178322	24.54941475258683
42	25.364572198699925	25.250986986653164	25.186583788980858	24.19785702566605
43	24.882055628134093	24.757265587473114	25.487992926397208	24.872685857995588
44	24.55601987599113	25.225092578322045	25.235227146262467	24.983660399424355
45	24.796451039256553	25.42853318545997	25.125685985811806	24.649329789471672
46	24.64301749174135	25.119835523019816	25.179839332057025	25.05730765318181
47	24.85027947830716	25.17863207070939	25.378106461807693	24.59298198917576
48	25.356280183999058	25.4936140333701	24.690530351124906	24.459575431505936
49	24.794938397383532	25.557398891856586	24.908100889390646	24.73956182136924
50	24.873607386682618	25.588060276847624	24.86469680305086	24.673635533418896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	59.0
2	65.0
3	169.5
4	274.0
5	313.5
6	353.0
7	296.0
8	239.0
9	318.0
10	397.0
11	686.5
12	976.0
13	1852.0
14	2728.0
15	3632.0
16	4536.0
17	4510.0
18	4484.0
19	4620.5
20	4757.0
21	5810.5
22	6864.0
23	8670.5
24	10477.0
25	13832.5
26	17188.0
27	21937.0
28	26686.0
29	30894.5
30	35103.0
31	39813.0
32	44523.0
33	51438.0
34	58353.0
35	66884.5
36	75416.0
37	86195.0
38	96974.0
39	106975.5
40	116977.0
41	127698.5
42	138420.0
43	145131.5
44	151843.0
45	163865.0
46	175887.0
47	185844.5
48	195802.0
49	201506.0
50	207210.0
51	202383.5
52	197557.0
53	194186.0
54	190815.0
55	189000.5
56	187186.0
57	179249.0
58	171312.0
59	156016.0
60	140720.0
61	123061.0
62	105402.0
63	91204.0
64	77006.0
65	64697.0
66	52388.0
67	45249.5
68	38111.0
69	33953.0
70	29795.0
71	23395.5
72	16996.0
73	14995.5
74	12995.0
75	9660.0
76	6325.0
77	5531.0
78	4737.0
79	3954.5
80	3172.0
81	2431.5
82	1691.0
83	1411.0
84	1131.0
85	848.5
86	566.0
87	393.5
88	221.0
89	146.5
90	72.0
91	55.0
92	38.0
93	33.0
94	28.0
95	24.0
96	20.0
97	22.0
98	24.0
99	18.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030173168748704543
2	0.004091925292916839
3	2.2945375474300032E-4
4	7.648458491433345E-5
5	0.0
6	1.529691698286669E-4
7	0.0
8	0.0
9	0.0
10	1.1472687737150016E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.795727265148346E-4
17	0.0
18	1.1472687737150016E-4
19	0.0
20	1.912114622858336E-4
21	3.8242292457166724E-5
22	0.0
23	7.648458491433345E-5
24	0.0011090264812578347
25	2.6769604720016705E-4
26	4.206652170288339E-4
27	4.971498019431674E-4
28	0.001797387745486836
29	3.824229245716672E-4
30	0.0014149648209151686
31	4.206652170288339E-4
32	8.413304340576678E-4
33	3.059383396573338E-4
34	6.883612642290011E-4
35	3.8242292457166724E-5
36	2.2945375474300032E-4
37	0.0017209031605725023
38	0.0
39	9.178150189720013E-4
40	7.648458491433345E-5
41	0.0011090264812578347
42	0.004933255726974507
43	0.004359621340117006
44	0.003556533198516505
45	0.0021415683776013364
46	0.0027534450569160042
47	0.0013002379435436686
48	0.00420665217028834
49	0.0031358679814876707
50	0.0014532071133723354
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2614906.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.888416583843664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32221322011397	40.589948040225984
2	14.56571006004998	15.69846103110912
3	4.490848713094402	7.260141787987481
4	1.842536634300464	3.9716552688070657
5	0.9389428611285177	2.529907202445982
6	0.5909745039549601	1.9108008155733154
7	0.3989680458666187	1.504982938151168
8	0.2863804523759237	1.2346071295282703
9	0.2127587861819363	1.0318710691480597
>10	1.2068968266983398	11.914923543404752
>50	0.08607367062247491	3.2072112054007924
>100	0.049639182233778334	5.316625583872838
>500	0.005846704044606762	2.153347182058645
>1k	0.0022103393339279517	1.6755172022865943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.824229245716672E-5	0.0
8	0.0	0.0	0.0	3.824229245716672E-5	0.0
9	0.0	0.0	0.0	3.824229245716672E-5	3.824229245716672E-5
10	0.0	0.0	0.0	3.824229245716672E-5	3.824229245716672E-5
11	0.0	0.0	0.0	3.824229245716672E-5	3.824229245716672E-5
12	0.0	0.0	0.0	3.824229245716672E-5	7.648458491433343E-5
13	0.0	0.0	0.0	7.648458491433343E-5	7.648458491433343E-5
14	0.0	0.0	0.0	7.648458491433343E-5	1.1472687737150016E-4
15	0.0	0.0	0.0	7.648458491433343E-5	1.5296916982866687E-4
16	0.0	0.0	0.0	7.648458491433343E-5	1.5296916982866687E-4
17	0.0	0.0	0.0	1.1472687737150016E-4	1.5296916982866687E-4
18	0.0	0.0	0.0	1.1472687737150016E-4	1.5296916982866687E-4
19	0.0	0.0	0.0	1.912114622858336E-4	1.5296916982866687E-4
20	0.0	0.0	0.0	2.6769604720016705E-4	1.912114622858336E-4
21	0.0	0.0	0.0	3.0593833965733374E-4	1.912114622858336E-4
22	0.0	0.0	0.0	4.5890750948600063E-4	1.912114622858336E-4
23	0.0	0.0	0.0	7.266035566861677E-4	3.0593833965733374E-4
24	0.0	0.0	0.0	0.0010707841888006682	3.0593833965733374E-4
25	0.0	0.0	0.0	0.001491449405829502	3.0593833965733374E-4
26	0.0	0.0	0.0	0.0018356300379440025	3.0593833965733374E-4
27	0.0	0.0	0.0	0.0021033260851441695	3.0593833965733374E-4
28	0.0	0.0	0.0	0.002485749009715837	3.0593833965733374E-4
29	0.0	0.0	0.0	0.003135867981487671	3.0593833965733374E-4
30	0.0	0.0	0.0	0.003977198415545339	3.0593833965733374E-4
31	0.0	0.0	0.0	0.0057745861610321745	3.441806321145005E-4
32	0.0	0.0	0.0	0.007571973906519011	3.441806321145005E-4
33	0.0	0.0	0.0	0.010019480623777681	3.441806321145005E-4
34	0.0	0.0	0.0	0.013155348605265352	3.824229245716672E-4
35	0.0	0.0	0.0	0.015526370737609688	3.824229245716672E-4
36	0.0	0.0	0.0	0.019274115398412027	3.824229245716672E-4
37	0.0	0.0	0.0	0.025813547408587535	3.824229245716672E-4
38	0.0	0.0	0.0	0.033538490484935214	3.824229245716672E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3005	0.0	28.486792	1
GGTATCA	1345	0.0	22.578506	1
CGCAATA	515	0.0	21.786795	36
TACGAAT	585	0.0	19.5574	41
CTAGCGG	605	0.0	18.908344	29
ATACGAA	595	0.0	18.85856	40
TCAACGC	4445	0.0	18.806904	4
AGTATCG	95	1.598813E-5	18.525583	8
ATCAACG	4525	0.0	18.474762	3
TCTAGCG	620	0.0	18.451235	28
CAACGCA	4540	0.0	18.41337	5
ACCGTCG	540	0.0	18.33261	8
AACGCAG	4590	0.0	18.308643	6
TATCAAC	4660	0.0	18.129425	2
GCGCAAT	630	0.0	17.80916	35
CGCCGGT	655	0.0	17.800823	7
TAGCGGC	645	0.0	17.735733	30
CGAATGC	645	0.0	17.396658	43
CGTCGTA	520	0.0	17.3458	10
GATATAC	470	0.0	17.323763	1
>>END_MODULE