FastQCFastQC Report
Thu 2 Feb 2017
SRR4062237_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062237_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164245
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCAC48542.9553411062741635No Hit
GTACATGGAAGCAGTGGTATCAACG43192.629608207251362No Hit
CCCATGTACTCTGCGTTGATACCAC20901.2724892690797285No Hit
GAGTACATGGAAGCAGTGGTATCAA20401.2420469420682516No Hit
CATGTACTCTGCGTTGATACCACTG17281.0520868215166366No Hit
GCTTCCATGTACTCTGCGTTGATAC15980.9729367712867972No Hit
GTACATGGGAAGCAGTGGTATCAAC15950.9711102316661085No Hit
GCGTTGATACCACTGCTTCCATGTA11710.7129592986087857No Hit
CATGGAAGCAGTGGTATCAACGCAG9850.5997138421260921No Hit
ACTCTGCGTTGATACCACTGCTTCC8820.5370026484824499No Hit
GTATCAACGCAGAGTACATGGAAGC7730.47063837559743066No Hit
TATCAACGCAGAGTACATGGAAGCA6440.3920971719078206No Hit
CAGTGGTATCAACGCAGAGTACATG6240.3799202411032299No Hit
GTATCAACGCAGAGTACATGGGAAG5850.3561752260342781No Hit
GAGTACATGGGAAGCAGTGGTATCA5790.35252214679290084No Hit
ACGCAGAGTACATGGAAGCAGTGGT5690.3464336813906055No Hit
ATACCACTGCTTCCATGTACTCTGC4970.302596730494079No Hit
AAAAAGTACTCTGCGTTGATACCAC4870.29650826509178363No Hit
GCTTCCCATGTACTCTGCGTTGATA4630.2818959481262748No Hit
TATCAACGCAGAGTACATGGGAAGC4490.2733720965630613No Hit
GTACTCTGCGTTGATACCACTGCTT4310.2624128588389296No Hit
AAGCAGTGGTATCAACGCAGAGTAC4060.24719169533319127No Hit
GCGTTGATACCACTGCTTCCCATGT3930.23927669031020732No Hit
CCACTGCTTCCATGTACTCTGCGTT3870.2356236110688301No Hit
GTGGTATCAACGCAGAGTACATGGA3530.2149228287010259No Hit
GTACATGGAAGCAGTGGTATCAACC3510.21370513562056684No Hit
GGTATCAACGCAGAGTACATGGAAG3440.2094432098389601No Hit
ACGCAGAGTACATGGGAAGCAGTGG3190.1942220463332217No Hit
GAAGCAGTGGTATCAACGCAGAGTA3170.19300435325276263No Hit
ACATGGAAGCAGTGGTATCAACGCA2900.1765654966665652No Hit
GGTATCAACGCAGAGTACATGGGAA2810.17108587780449935No Hit
AAAGTACTCTGCGTTGATACCACTG2530.1540381746780724No Hit
TGGAAGCAGTGGTATCAACGCAGAG2510.15282048159761333No Hit
GTATCAACGCAGAGTACTTTTTTTT2500.15221163505738378No Hit
GTACTTTTTTTTTTTTTTTTTTTTT2340.14247009041371123No Hit
CCATGTACTCTGCGTTGATACCACT2330.14186124387348167No Hit
GATACCACTGCTTCCATGTACTCTG2310.14064355079302263No Hit
CTGCTTCCATGTACTCTGCGTTGAT2290.13942585771256355No Hit
CCCCATGTACTCTGCGTTGATACCA2180.13272854577003865No Hit
GTTGATACCACTGCTTCCATGTACT1960.11933392188498888No Hit
GCAGTGGTATCAACGCAGAGTACAT1950.11872507534475936No Hit
TATCAACGCAGAGTACTTTTTTTTT1820.1108100703217754No Hit
CTTCCATGTACTCTGCGTTGATACC1740.10593929799993912No Hit
CTGCTTCCCATGTACTCTGCGTTGA1650.10045967913787329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGAG753.2196112E-916.46270218
TTTTTCA508.6562075E-515.19634218
GGCGAGA653.346824E-614.60741319
TATGGCG851.6781996E-814.53034516
ATGGCGA851.6849299E-814.52591517
GAAGGAC707.186147E-613.5681613
AAGGACC655.3760297E-513.1587044
AAAAAGT905.342208E-712.6597581
GACCTGG701.07575186E-412.2187967
CTGGAAT802.8280498E-511.87576210
AGGACCT752.0452985E-411.404215
GGACCTG752.0452985E-411.404216
ATCAACC752.0627736E-411.39378219
ACATTTC700.001478322910.8578413
ATATGGC1408.083589E-910.8578415
AAAAGTA1053.4267305E-610.8512212
TGAAGGA803.750949E-410.6816712
CATTTCT951.6225084E-410.00064214
AATATGG1553.9352017E-89.80708114
GGGAAGC4050.09.3861817
Produced by FastQC (version 0.11.5)