##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062237_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	164245
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.583335870193917	32.0	32.0	32.0	32.0	32.0
2	30.356467472373588	32.0	32.0	32.0	21.0	32.0
3	31.03246369752504	32.0	32.0	32.0	32.0	32.0
4	31.296216018752474	32.0	32.0	32.0	32.0	32.0
5	31.171372035678406	32.0	32.0	32.0	32.0	32.0
6	34.793582757465984	36.0	36.0	36.0	32.0	36.0
7	34.79251118755518	36.0	36.0	36.0	32.0	36.0
8	34.75548114097842	36.0	36.0	36.0	32.0	36.0
9	34.92650613412889	36.0	36.0	36.0	32.0	36.0
10	34.64542604036652	36.0	36.0	36.0	32.0	36.0
11	34.945343846083595	36.0	36.0	36.0	32.0	36.0
12	34.76677524429967	36.0	36.0	36.0	32.0	36.0
13	34.85678102834181	36.0	36.0	36.0	32.0	36.0
14	34.7978629486438	36.0	36.0	36.0	32.0	36.0
15	34.73400712350452	36.0	36.0	36.0	32.0	36.0
16	34.73437851989406	36.0	36.0	36.0	32.0	36.0
17	34.69313525525891	36.0	36.0	36.0	32.0	36.0
18	34.70825900331821	36.0	36.0	36.0	32.0	36.0
19	34.637194435142625	36.0	36.0	36.0	32.0	36.0
20	34.6976041888642	36.0	36.0	36.0	32.0	36.0
21	34.591129105908855	36.0	36.0	36.0	32.0	36.0
22	34.49218545465615	36.0	36.0	36.0	32.0	36.0
23	34.33134646412372	36.0	36.0	36.0	32.0	36.0
24	34.34766963986727	36.0	36.0	36.0	32.0	36.0
25	34.34796188620658	36.0	36.0	36.0	32.0	36.0
26	34.273560838990534	36.0	36.0	36.0	32.0	36.0
27	34.219069073639986	36.0	36.0	36.0	32.0	36.0
28	34.136058936345094	36.0	36.0	36.0	32.0	36.0
29	34.09561934914305	36.0	36.0	36.0	32.0	36.0
30	34.08153064020214	36.0	36.0	36.0	32.0	36.0
31	33.99904411093184	36.0	36.0	36.0	32.0	36.0
32	33.94652500837164	36.0	36.0	36.0	32.0	36.0
33	33.863941063654906	36.0	36.0	36.0	32.0	36.0
34	33.76229413376358	36.0	36.0	36.0	32.0	36.0
35	33.67289719626168	36.0	36.0	36.0	27.0	36.0
36	33.61376602027459	36.0	36.0	36.0	27.0	36.0
37	33.483485037596274	36.0	36.0	36.0	21.0	36.0
38	33.31825017504338	36.0	36.0	36.0	21.0	36.0
39	32.98197205394381	36.0	36.0	36.0	14.0	36.0
40	32.78866327742092	36.0	36.0	36.0	14.0	36.0
41	32.72822916983774	36.0	36.0	36.0	14.0	36.0
42	32.55281439313221	36.0	36.0	36.0	14.0	36.0
43	32.539084903650036	36.0	36.0	36.0	14.0	36.0
44	32.603726140826204	36.0	36.0	36.0	14.0	36.0
45	32.20856038235563	36.0	32.0	36.0	14.0	36.0
46	32.39857529909586	36.0	36.0	36.0	14.0	36.0
47	32.33912143444245	36.0	36.0	36.0	14.0	36.0
48	32.206429419464826	36.0	32.0	36.0	14.0	36.0
49	32.423854607446195	36.0	36.0	36.0	14.0	36.0
50	31.487497336296386	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	8.0
22	30.0
23	68.0
24	174.0
25	458.0
26	962.0
27	1705.0
28	3116.0
29	4742.0
30	7547.0
31	11961.0
32	18249.0
33	27122.0
34	40051.0
35	48049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.885935263847024	17.034195061051737	13.384488901068787	23.695380774032458
2	12.809459443003446	22.55324589924378	36.87818896966597	27.759105688086798
3	19.618253219276085	27.922311181466714	27.757922615604734	24.70151298365247
4	13.026271728210903	18.750038052908767	34.20134555085391	34.02234466802642
5	13.225364546865961	38.22216810252976	33.37818502846358	15.174282322140703
6	33.430545830923315	32.82291698377424	18.63009528448355	15.1164419008189
7	27.5819659654784	31.86702791561387	22.284392218941214	18.266613899966515
8	29.20880392097172	32.36567323206186	21.447228226125606	16.978294620840817
9	27.65259216414503	16.697007519254772	17.427014520989985	38.22338579561021
10	15.666934560775431	29.027544385183017	32.79998051679209	22.50554053724946
11	34.732259733934065	22.784255228469664	20.004870772321837	22.478614265274437
12	22.52975737465372	25.06803860087065	29.524186428810616	22.878017595665014
13	32.21894121586654	21.02103564796493	23.542269171055434	23.217753965113094
14	25.28174373649122	19.496483911230175	23.95445827879083	31.26731407348778
15	27.013303296904017	24.75326493957198	26.907972845444306	21.325458918079697
16	26.253462814697553	24.79527535084782	27.173429936984384	21.777831897470243
17	24.879296173399496	25.021766263813205	28.688240129075464	21.410697433711835
18	27.010423516231945	22.14266578992231	29.946908258444754	20.90000243540099
19	23.56114341380255	31.33063411367165	25.04307589272124	20.065146579804562
20	23.297654708847812	23.194759017072162	32.323859623487	21.18372665059302
21	23.513044537124415	28.141495935949344	22.823830253584585	25.521629273341656
22	22.204633322171148	28.91899296782246	23.676824256446164	25.199549453560234
23	21.234131937045266	27.902219245639138	23.595847666595635	27.26780115071996
24	21.368598583788454	30.693310440145883	23.810741532260522	24.12734944380514
25	26.412532573488225	24.44655512530138	22.034899296169115	27.10601300504128
26	21.373818062261403	24.45401021656935	25.770961319508288	28.401210401660954
27	26.73355942110166	27.500106549441984	24.28353110939279	21.482802920063566
28	24.85159184866326	23.023203424194637	25.247346918894564	26.877857808247533
29	22.969344576699445	27.375566988340587	28.05321318761606	21.60187524734391
30	27.982148510752296	23.746377341029202	26.760186064635544	21.51128808358296
31	22.63143474687205	24.956619684008647	26.19684008645621	26.21510548266309
32	21.772484839628845	27.425659384817706	26.743747107961326	24.05810866759212
33	21.735212639654176	26.448293707571008	26.422722152881366	25.393771499893454
34	20.40975372157448	28.48305884501811	28.30223142256994	22.80495601083747
35	24.627233705744466	26.763676215409905	26.374014429663	22.235075649182622
36	24.364820846905538	27.37617583488082	26.10307771926086	22.155925598952784
37	25.902924916587516	26.615888556050756	26.1464650154648	21.334721511896934
38	24.83302383634205	26.961551340984506	22.912113001917866	25.293311820755576
39	22.975433042101738	26.71679503181223	26.68391731863984	23.623854607446194
40	26.055904970074828	26.499150648733888	22.928831061293327	24.516113319897954
41	21.664850252369963	26.40904026351199	26.248302819602664	25.677806664515384
42	27.93828430865152	27.018272374678055	24.471343241778648	20.57210007489177
43	26.312296404542273	23.122964045422716	23.768989557646055	26.795749992388956
44	25.017809411893644	24.90273321196549	28.888997132228035	21.190460243912835
45	28.062687603124637	24.587958086493796	25.555427019720778	21.79392729066079
46	24.015489150288595	24.701054528628138	29.04398334185724	22.23947297922603
47	27.456877918693646	23.272224691463258	28.270915655461724	20.99998173438137
48	23.323753622835433	31.172824472101123	23.97951728001169	21.523904625051753
49	22.23203195401739	24.346671862441852	32.08918872841521	21.332107455125552
50	22.72054749480939	30.161532888047297	22.29981916597154	24.81810045117177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	18.0
2	18.0
3	91.5
4	165.0
5	142.5
6	120.0
7	110.0
8	100.0
9	109.0
10	118.0
11	151.0
12	184.0
13	257.5
14	331.0
15	385.0
16	439.0
17	483.0
18	527.0
19	534.0
20	541.0
21	692.0
22	843.0
23	889.5
24	936.0
25	1129.0
26	1322.0
27	1592.0
28	1862.0
29	2185.0
30	2508.0
31	2665.0
32	2822.0
33	3295.0
34	3768.0
35	4333.0
36	4898.0
37	5410.5
38	5923.0
39	6071.0
40	6219.0
41	6596.5
42	6974.0
43	7526.0
44	8078.0
45	12010.5
46	15943.0
47	20056.5
48	24170.0
49	21452.5
50	18735.0
51	15112.5
52	11490.0
53	9925.0
54	8360.0
55	8112.0
56	7864.0
57	7492.5
58	7121.0
59	6661.5
60	6202.0
61	5418.0
62	4634.0
63	4061.0
64	3488.0
65	2945.5
66	2403.0
67	2071.0
68	1739.0
69	1462.0
70	1185.0
71	993.5
72	802.0
73	672.0
74	542.0
75	441.5
76	341.0
77	265.0
78	189.0
79	171.5
80	154.0
81	120.0
82	86.0
83	59.5
84	33.0
85	27.5
86	22.0
87	15.0
88	8.0
89	8.5
90	9.0
91	5.5
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	3.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024353861609181404
2	0.004261925781606746
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	6.088465402295352E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	6.088465402295352E-4
19	0.0
20	6.088465402295352E-4
21	0.0
22	0.0
23	0.0
24	0.0024353861609181407
25	6.088465402295352E-4
26	0.0012176930804590703
27	0.0012176930804590703
28	0.0012176930804590703
29	0.0
30	6.088465402295352E-4
31	0.0
32	6.088465402295352E-4
33	0.0
34	0.0
35	0.0
36	0.0
37	6.088465402295352E-4
38	0.0
39	0.0
40	0.0012176930804590703
41	0.0012176930804590703
42	0.004870772321836281
43	0.006088465402295351
44	0.003653079241377211
45	0.0012176930804590703
46	6.088465402295352E-4
47	0.0012176930804590703
48	0.005479618862065817
49	0.005479618862065817
50	0.003653079241377211
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	164245.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.98469309214182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.12146974781622	55.10455810458198
2	9.378273088104622	12.001318505533348
3	2.0298168376407717	3.8963162216911993
4	0.6935843730168471	1.775151329639543
5	0.4197309316583497	1.342817742171913
6	0.2528370508855157	0.9706620661939179
7	0.16789174933328144	0.7519751437654973
8	0.16189561168313973	0.8287072821208213
9	0.09294007319711396	0.535206785353068
>10	0.5986140197388755	6.945538950445982
>50	0.03397809127059136	1.4176265745832213
>100	0.03597680252180262	4.706878311009062
>500	0.008994200630450654	4.178065872046354
>1k	0.003997422502422513	5.545177110864093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3180	1.9361319979299216	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3124	1.9020365916770678	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1348	0.8207251362294135	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1020	0.6210234710341258	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	988	0.6015403817467807	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	930	0.5662272824134676	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	868	0.5284787969192365	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	780	0.47490030137903744	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	698	0.4249748850802155	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	677	0.4121891077353953	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	547	0.33303905750555574	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	540	0.328777131723949	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	506	0.3080763493561448	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	405	0.24658284879296175	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	376	0.22892629912630524	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	374	0.22770860604584614	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	354	0.21553167524125547	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	354	0.21553167524125547	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	321	0.19543973941368079	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	314	0.19117781363207403	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	294	0.17900088282748333	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	284	0.17291241742518798	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	264	0.16073548662059728	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	236	0.1436877834941703	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA	224	0.13638162501141587	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	210	0.12785777344820237	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	203	0.12359584766659563	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA	192	0.11689853572407075	No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG	188	0.11446314956315262	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	188	0.11446314956315262	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG	187	0.11385430302292307	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	185	0.112636609942464	No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	175	0.10654814454016866	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	172	0.10472160491948004	No Hit
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT	170	0.10350391183902097	No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	170	0.10350391183902097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	6.088465402295352E-4	0.0
22	0.0	0.0	0.0	6.088465402295352E-4	0.0
23	0.0	0.0	0.0	6.088465402295352E-4	0.0
24	0.0	0.0	0.0	6.088465402295352E-4	0.0
25	0.0	0.0	0.0	6.088465402295352E-4	0.0
26	0.0	0.0	0.0	6.088465402295352E-4	0.0
27	0.0	0.0	0.0	6.088465402295352E-4	0.0
28	0.0	0.0	0.0	6.088465402295352E-4	0.0
29	0.0	0.0	0.0	0.0012176930804590703	0.0
30	0.0	0.0	0.0	0.0018265396206886054	0.0
31	0.0	0.0	0.0	0.004261925781606746	0.0
32	0.0	0.0	0.0	0.006697311942524886	0.0
33	0.0	0.0	0.0	0.009132698103443027	0.0
34	0.0	0.0	0.0	0.012785777344820238	6.088465402295352E-4
35	0.0	0.0	0.0	0.015830010045967915	6.088465402295352E-4
36	0.0	0.0	0.0	0.020700782367804194	6.088465402295352E-4
37	0.0	0.0	0.0	0.028615787390788152	6.088465402295352E-4
38	0.0	0.0	0.0	0.044445797436756064	0.0012176930804590703
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCCT	30	1.2962421E-4	36.666668	44
GCGTAGA	25	0.0023468959	35.2	43
TCACGTC	25	0.0023468959	35.2	42
TGGTATA	145	0.0	31.862068	44
CACGTCC	30	0.0057307505	29.333336	43
AAAAAGT	105	3.0213414E-9	23.047619	1
GTCCTAC	70	3.1935888E-5	22.0	1
CCTACAG	70	3.1935888E-5	22.0	3
ATTGTCT	50	0.0025710412	22.0	3
TGCACTG	50	0.0025710412	22.0	5
TGTAGGA	90	4.0375744E-7	21.999998	2
CTGGAAT	65	4.9072446E-4	20.307693	10
TTGTCTT	55	0.004467416	20.0	4
TTCTAAA	70	8.0825645E-4	18.857143	17
AAAGTAC	140	4.0054147E-9	18.857143	3
ATTTCTA	70	8.0825645E-4	18.857143	15
TCACGGA	60	0.007385541	18.333334	36
CACGGAA	60	0.007385541	18.333334	37
ATCACGG	60	0.007385541	18.333334	35
GGCTGGT	60	0.007385541	18.333334	7
>>END_MODULE