FastQCFastQC Report
Thu 2 Feb 2017
SRR4062236_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062236_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87629
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG12951.4778212692145294No Hit
TCCATGTACTCTGCGTTGATACCAC10371.1833981900968857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9701.1069394835043194No Hit
GTACATGGGAAGCAGTGGTATCAAC7410.845610471419279No Hit
CCCATGTACTCTGCGTTGATACCAC5840.666446039553116No Hit
GTATCAACGCAGAGTACTTTTTTTT5720.6527519428499697No Hit
GAGTACATGGAAGCAGTGGTATCAA5630.6424813703226101No Hit
CATGTACTCTGCGTTGATACCACTG4970.5671638384553059No Hit
GTATCAACGCAGAGTACATGGGAAG4400.5021168791153613No Hit
GTCCTAAAGTGTGTATTTCTCATTT4250.48499925823642864No Hit
TATCAACGCAGAGTACTTTTTTTTT4170.4758698604343311No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4140.4724463362585445No Hit
CTGTAGGACGTGGAATATGGCAAGA4060.4633169384564471No Hit
GTCCTACAGTGGACATTTCTAAATT3880.4427757934017278No Hit
GCTTCCATGTACTCTGCGTTGATAC3790.43250522087436805No Hit
CTTTAGGACGTGAAATATGGCGAGG3460.394846454940716No Hit
CATGGAAGCAGTGGTATCAACGCAG3030.34577594175444204No Hit
GCGTTGATACCACTGCTTCCATGTA3020.34463476702917983No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2830.3229524472491983No Hit
GGTATCAACGCAGAGTACATGGGAA2730.3115406999965765No Hit
TATCAACGCAGAGTACATGGGAAGC2640.30127012746921683No Hit
GTCCTACAGTGTGCATTTCTCATTT2640.30127012746921683No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2630.3001289527439546No Hit
GATATACACTGTTCTACAAATCCCG2520.2875760307660706No Hit
CCATAGGGTCTTCTCGTCTTATTAT2490.284152506590284No Hit
GGTATCAACGCAGAGTACTTTTTTT2430.2773054582387109No Hit
ACTCTGCGTTGATACCACTGCTTCC2370.2704584098871378No Hit
CAGTGGTATCAACGCAGAGTACATG2140.24421139120610758No Hit
GTGTATATCAATGAGTTACAATGAA2130.24307021648084537No Hit
GTATCAACGCAGAGTACATGGAAGC2080.23736434285453445No Hit
GAGTACATGGGAAGCAGTGGTATCA2000.228234945052437No Hit
GCGTTGATACCACTGCTTCCCATGT1990.2270937703271748No Hit
TTGTAGAACAGTGTATATCAATGAG1950.22252907142612605No Hit
GTGTATATCAATGAGTTACAATGAG1880.21454084834929077No Hit
CTGTAGGACCTGGAATATGGCGAGA1850.2111173241735042No Hit
GCTTCCCATGTACTCTGCGTTGATA1810.20655262527245546No Hit
TATCAACGCAGAGTACATGGAAGCA1770.20198792637140672No Hit
ACGCAGAGTACATGGGAAGCAGTGG1750.19970557692088237No Hit
GTATTAGAGGCACTGCCTGCCCAGT1710.19514087801983362No Hit
CACTATAAATAATCCACCTATAACT1580.1803056065914252No Hit
ACGCAGAGTACATGGAAGCAGTGGT1550.17688208241563866No Hit
ACCTATAACTTCTCTGTTAACCCAA1530.1745997329651143No Hit
CTGAAGGACCTGGAATATGGCGAGA1500.17117620878932774No Hit
CCATTGGGATGTCCTGATCCAACAT1490.17003503406406553No Hit
GTGGTATCAACGCAGAGTACATGGG1450.16547033516301682No Hit
GTACATGGGGTGGTATCAACGCAAA1440.16432916043775464No Hit
GTCCTTCAGTGTGCATTTCTCATTT1440.16432916043775464No Hit
ATTTAGAAATGTCCACTGTAGGACG1440.16432916043775464No Hit
GTCAGGATACCGCGGCCGTTAAACT1430.16318798571249243No Hit
GTATCAACGCAGAGTACATGGGAGA1400.15976446153670587No Hit
GACTATAGGCAATAATCACACTATA1390.1586232868114437No Hit
GTTCTACAGTGTGGTTTTTATCATT1370.15634093736091934No Hit
GTACATGGGAGTGGTATCAACGCAA1360.15519976263565716No Hit
GTTATAGATTAACCCAATTTTAAGT1350.15405858791039498No Hit
ATCTATAACTTTATAGATGCAACAC1340.15291741318513277No Hit
GGTATCAACGCAGAGTACATGGAAG1330.1517762384598706No Hit
AAAAAGTACTCTGCGTTGATACCAC1330.1517762384598706No Hit
ATAAATAATCCACCTATAACTTCTC1320.15063506373460842No Hit
CTCTAATACTTGTAATGCTAGAGGT1310.1494938890093462No Hit
CTATAGAACTAGTACCGCAAGGGAA1310.1494938890093462No Hit
GTATCCTGACCGTGCAAAGGTAGCA1300.14835271428408403No Hit
GTTCTACAAATCCCGTTTCCAACGA1290.14721153955882185No Hit
GCCTAAAGGAAAGATCCAAAAAGAT1290.14721153955882185No Hit
GTTATATAATTTAAGCTCCATAGGG1280.14607036483355967No Hit
GATTAAACCTTGTACCTTTTGCATA1280.14607036483355967No Hit
ATCCTGACCGTGCAAAGGTAGCATA1230.14036449120724873No Hit
GTTCATGCTAGTCCCTAATTAAGGA1230.14036449120724873No Hit
GAATATGGCAAGAAAACTGAAAATC1230.14036449120724873No Hit
ATCGTAAATAGATAGAAACCGACCT1200.1369409670314622No Hit
CTGTTAACCCAACACCGGAATGCCT1180.13465861758093783No Hit
GTACTCTGCGTTGATACCACTGCTT1180.13465861758093783No Hit
GGTCAGGATACCGCGGCCGTTAAAC1170.13351744285567563No Hit
CCTATAACTTCTCTGTTAACCCAAC1170.13351744285567563No Hit
GAAGCAGTGGTATCAACGCAGAGTA1170.13351744285567563No Hit
GTGGTATCAACGCAGAGTACATGGA1150.13123509340515127No Hit
CTTCTACACCATTGGGATGTCCTGA1130.12895274395462689No Hit
GATTAAAGATAAGAGACAGTTGGAC1120.1278115692293647No Hit
TTTCTAAATTTTCCACCTTTTTCAG1120.1278115692293647No Hit
CTGTTAGTATGAGTAACAAGAATTC1090.12438804505357816No Hit
CTGTAGAACATATTAGATGAGTGAG1060.12096452087779161No Hit
CTATTAAAGGTTTTTTCCGTTCCAG1060.12096452087779161No Hit
ATGTTCTACAGTGTGGTTTTTATCA1060.12096452087779161No Hit
CCTCTAGCATTACAAGTATTAGAGG1030.11754099670200505No Hit
AAGCAGTGGTATCAACGCAGAGTAC1030.11754099670200505No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1020.11639982197674285No Hit
GCTATCACCAAGCTCGTTAGGCTTT1000.1141174725262185No Hit
TGCTAGAGGTGATGTTTTTGGTAAA1000.1141174725262185No Hit
ACATGGAAGCAGTGGTATCAACGCA990.11297629780095629No Hit
CTTTATTGGTGGCTGCTTTTAGGCC970.11069394835043195No Hit
GATATACACTGTTCTACAATGCCGG960.10955277362516974No Hit
GTTTAAAATTGAACTTAAATTCATT960.10955277362516974No Hit
GGTATCAACGCAGAGTACATGGGAG950.10841159889990756No Hit
GGAATATGGCGAGAAAACTGAAAAT950.10841159889990756No Hit
AAAGTACTCTGCGTTGATACCACTG940.10727042417464538No Hit
GTACATGGGAGAAATCGTAAATAGA940.10727042417464538No Hit
GTATCAACGCAGAGTACATGGGAGT930.10612924944938319No Hit
AATCTAAACTTACTTTTTGATTTTG920.10498807472412101No Hit
TACTAACAGTGTTGCATCTATAAAG900.10270572527359663No Hit
GATAGAAACCGACCTGGATTGCTCC890.10156455054833445No Hit
ATTCCAGGTCCTTCAGTGTGCATTT890.10156455054833445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATTA250.005984545719.0017156
GAAATGT504.4600056E-617.1015436
TTTAGAA504.4844146E-617.091772
ATTTAGA504.5089437E-617.082011
GAACAGT453.4749915E-516.8904136
AACTCAG350.002146960516.28718617
ACTCAGA350.002146960516.28718618
TCCGGTC350.002146960516.2871868
TGCTCCG350.002146960516.2871865
CTCCGGT350.002146960516.2871867
CCGGTCT350.002146960516.2871869
TTAGAAA551.1032815E-515.5468593
AGTGTAT551.1032815E-515.54685910
AATGTCC551.1032815E-515.5468598
GGTCTGA400.00522344714.25128711
CTGAACT400.00522344714.25128714
GAACTCA400.00522344714.25128716
TCTGAAC400.00522344714.25128713
GCTCCGG400.00522344714.2512876
TGAACTC400.00522344714.25128715
Produced by FastQC (version 0.11.5)