##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062236_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87629
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.880370653550766	32.0	32.0	32.0	32.0	32.0
2	30.803113124650515	32.0	32.0	32.0	32.0	32.0
3	30.85484257494665	32.0	32.0	32.0	32.0	32.0
4	30.774024580903582	32.0	32.0	32.0	32.0	32.0
5	30.982220497780414	32.0	32.0	32.0	32.0	32.0
6	34.48566113957708	36.0	36.0	36.0	32.0	36.0
7	34.26999052824978	36.0	36.0	36.0	32.0	36.0
8	34.17179244314097	36.0	36.0	36.0	32.0	36.0
9	34.11917287655913	36.0	36.0	36.0	32.0	36.0
10	34.128998390943636	36.0	36.0	36.0	32.0	36.0
11	34.287690148238596	36.0	36.0	36.0	32.0	36.0
12	34.167855390338815	36.0	36.0	36.0	32.0	36.0
13	34.11199488753723	36.0	36.0	36.0	32.0	36.0
14	34.0583140284609	36.0	36.0	36.0	32.0	36.0
15	34.06031108423011	36.0	36.0	36.0	32.0	36.0
16	34.02439831562611	36.0	36.0	36.0	32.0	36.0
17	33.982197674285906	36.0	36.0	36.0	32.0	36.0
18	33.96956487007726	36.0	36.0	36.0	32.0	36.0
19	33.881386299056246	36.0	36.0	36.0	32.0	36.0
20	33.771445526024486	36.0	36.0	36.0	27.0	36.0
21	33.679638019377144	36.0	36.0	36.0	27.0	36.0
22	33.62715539376234	36.0	36.0	36.0	27.0	36.0
23	33.578963585114515	36.0	36.0	36.0	27.0	36.0
24	33.53650047358751	36.0	36.0	36.0	27.0	36.0
25	32.98154720469251	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	0.0
5	9.0
6	16.0
7	3.0
8	18.0
9	16.0
10	4.0
11	4.0
12	4.0
13	0.0
14	186.0
15	189.0
16	187.0
17	219.0
18	243.0
19	285.0
20	307.0
21	363.0
22	451.0
23	550.0
24	694.0
25	714.0
26	903.0
27	1178.0
28	1404.0
29	1924.0
30	2590.0
31	3588.0
32	5092.0
33	7262.0
34	16611.0
35	42613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57722899953212	21.856919512946625	12.97401545150578	22.591836036015476
2	10.55586620867521	24.02058679204373	44.396261511600045	21.02728548768102
3	18.593371981558406	30.686538549322133	30.363582416579177	20.356507052540284
4	9.924901275993516	20.7902485790591	39.5318313588532	29.75301878609418
5	8.705181329406528	43.356962661096084	36.11291850735706	11.824937502140338
6	25.952995454856907	40.557979124317654	20.6107393280497	12.878286092775735
7	22.842193051604575	36.55390661594178	22.62855445499309	17.97534587746056
8	23.393389619440413	40.163831415873595	21.40204960641616	15.04072935826983
9	25.518738574040217	18.45749542961609	21.206581352833638	34.81718464351006
10	14.855784613978152	31.755953741372217	34.02774603464826	19.36051561000137
11	29.567582392466974	25.97819627919733	25.88106230287516	18.573159025460527
12	22.612163737343725	29.473407537768836	32.491486298082414	15.422942426805019
13	28.466961459797304	25.253944857688044	27.326637644397216	18.95245603811744
14	19.650141108990983	27.946435712571837	27.67107322813953	24.732349950297642
15	22.086871064690488	33.882229256419336	27.64401375858483	16.386885920305343
16	18.89668411069723	30.10923467172467	33.20231266710848	17.791768550469616
17	17.486288848263253	31.630484460694696	32.46115173674588	18.42207495429616
18	18.284225799449324	28.520833095316984	37.292782963360715	15.90215814187298
19	20.649988576650674	31.706648389307745	29.55220470641992	18.09115832762166
20	19.66188817179736	30.86298475069964	33.98138100405505	15.493746073447943
21	19.605021188134643	30.408114313127506	27.698774400621367	22.288090098116484
22	18.994528278178223	34.973326784021204	28.021155801281683	18.010989136518887
23	17.567243446976185	34.127120095939695	29.372394494888926	18.93324196219519
24	19.045987252415873	34.16718068215567	30.15214858474402	16.63468348068444
25	20.54825813820674	31.49971444888635	29.78640776699029	18.16561964591662
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1080.0
1	1080.0
2	680.0
3	280.0
4	280.0
5	280.0
6	534.5
7	789.0
8	789.0
9	789.0
10	707.5
11	626.0
12	626.0
13	626.0
14	663.5
15	701.0
16	701.0
17	701.0
18	1140.0
19	1579.0
20	1579.0
21	1579.0
22	2478.0
23	3377.0
24	3377.0
25	3377.0
26	5267.5
27	7158.0
28	7158.0
29	7158.0
30	8866.5
31	10575.0
32	10575.0
33	10575.0
34	10547.5
35	10520.0
36	10520.0
37	10520.0
38	10072.5
39	9625.0
40	9625.0
41	9625.0
42	9764.0
43	9903.0
44	9903.0
45	9903.0
46	13520.5
47	17138.0
48	17138.0
49	17138.0
50	12926.5
51	8715.0
52	8715.0
53	8715.0
54	5886.0
55	3057.0
56	3057.0
57	3057.0
58	2136.0
59	1215.0
60	1215.0
61	1215.0
62	887.5
63	560.0
64	560.0
65	560.0
66	442.0
67	324.0
68	324.0
69	324.0
70	257.0
71	190.0
72	190.0
73	190.0
74	151.5
75	113.0
76	113.0
77	113.0
78	82.0
79	51.0
80	51.0
81	51.0
82	35.0
83	19.0
84	19.0
85	19.0
86	13.0
87	7.0
88	7.0
89	7.0
90	4.5
91	2.0
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	12.5
99	24.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001141174725262185
4	0.012552921977884034
5	0.029670542856816806
6	0.07189400769151764
7	0.11183512307569411
8	0.1141174725262185
9	0.12438804505357816
10	0.13808214175672437
11	0.13808214175672437
12	0.14036449120724873
13	0.12552921977884032
14	0.12324687032831597
15	0.1369409670314622
16	0.1266703945041025
17	0.12438804505357816
18	0.1141174725262185
19	0.10156455054833445
20	0.09585867692202353
21	0.09129397802097479
22	0.10042337582307227
23	0.08444692966940168
24	0.09471750219676135
25	0.0901528032957126
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	87629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.9758641545607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.77415250089726	27.202181926074704
2	10.907370550425789	7.629894213102968
3	3.3182159287415574	3.481724086774926
4	1.526966622075761	2.13627908569081
5	1.0571307383601423	1.8487030549247396
6	0.606871349799341	1.2735509933925984
7	0.5611928611047668	1.3739743692156705
8	0.4567848869457405	1.278115692293647
9	0.29364742732226173	0.9243515274623698
>10	2.861431041795817	20.876650423946412
>50	0.3589024111716532	8.48235173287382
>100	0.25449443701262686	16.91677412728663
>500	0.016313745962347876	3.9142293076492938
>1k	0.006525498384939149	2.661219459311415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	1295	1.4778212692145294	No Hit
TCCATGTACTCTGCGTTGATACCAC	1037	1.1833981900968857	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	970	1.1069394835043194	No Hit
GTACATGGGAAGCAGTGGTATCAAC	741	0.845610471419279	No Hit
CCCATGTACTCTGCGTTGATACCAC	584	0.666446039553116	No Hit
GTATCAACGCAGAGTACTTTTTTTT	572	0.6527519428499697	No Hit
GAGTACATGGAAGCAGTGGTATCAA	563	0.6424813703226101	No Hit
CATGTACTCTGCGTTGATACCACTG	497	0.5671638384553059	No Hit
GTATCAACGCAGAGTACATGGGAAG	440	0.5021168791153613	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	425	0.48499925823642864	No Hit
TATCAACGCAGAGTACTTTTTTTTT	417	0.4758698604343311	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	414	0.4724463362585445	No Hit
CTGTAGGACGTGGAATATGGCAAGA	406	0.4633169384564471	No Hit
GTCCTACAGTGGACATTTCTAAATT	388	0.4427757934017278	No Hit
GCTTCCATGTACTCTGCGTTGATAC	379	0.43250522087436805	No Hit
CTTTAGGACGTGAAATATGGCGAGG	346	0.394846454940716	No Hit
CATGGAAGCAGTGGTATCAACGCAG	303	0.34577594175444204	No Hit
GCGTTGATACCACTGCTTCCATGTA	302	0.34463476702917983	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	283	0.3229524472491983	No Hit
GGTATCAACGCAGAGTACATGGGAA	273	0.3115406999965765	No Hit
TATCAACGCAGAGTACATGGGAAGC	264	0.30127012746921683	No Hit
GTCCTACAGTGTGCATTTCTCATTT	264	0.30127012746921683	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	263	0.3001289527439546	No Hit
GATATACACTGTTCTACAAATCCCG	252	0.2875760307660706	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	249	0.284152506590284	No Hit
GGTATCAACGCAGAGTACTTTTTTT	243	0.2773054582387109	No Hit
ACTCTGCGTTGATACCACTGCTTCC	237	0.2704584098871378	No Hit
CAGTGGTATCAACGCAGAGTACATG	214	0.24421139120610758	No Hit
GTGTATATCAATGAGTTACAATGAA	213	0.24307021648084537	No Hit
GTATCAACGCAGAGTACATGGAAGC	208	0.23736434285453445	No Hit
GAGTACATGGGAAGCAGTGGTATCA	200	0.228234945052437	No Hit
GCGTTGATACCACTGCTTCCCATGT	199	0.2270937703271748	No Hit
TTGTAGAACAGTGTATATCAATGAG	195	0.22252907142612605	No Hit
GTGTATATCAATGAGTTACAATGAG	188	0.21454084834929077	No Hit
CTGTAGGACCTGGAATATGGCGAGA	185	0.2111173241735042	No Hit
GCTTCCCATGTACTCTGCGTTGATA	181	0.20655262527245546	No Hit
TATCAACGCAGAGTACATGGAAGCA	177	0.20198792637140672	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	175	0.19970557692088237	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	171	0.19514087801983362	No Hit
CACTATAAATAATCCACCTATAACT	158	0.1803056065914252	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	155	0.17688208241563866	No Hit
ACCTATAACTTCTCTGTTAACCCAA	153	0.1745997329651143	No Hit
CTGAAGGACCTGGAATATGGCGAGA	150	0.17117620878932774	No Hit
CCATTGGGATGTCCTGATCCAACAT	149	0.17003503406406553	No Hit
GTGGTATCAACGCAGAGTACATGGG	145	0.16547033516301682	No Hit
GTACATGGGGTGGTATCAACGCAAA	144	0.16432916043775464	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	144	0.16432916043775464	No Hit
ATTTAGAAATGTCCACTGTAGGACG	144	0.16432916043775464	No Hit
GTCAGGATACCGCGGCCGTTAAACT	143	0.16318798571249243	No Hit
GTATCAACGCAGAGTACATGGGAGA	140	0.15976446153670587	No Hit
GACTATAGGCAATAATCACACTATA	139	0.1586232868114437	No Hit
GTTCTACAGTGTGGTTTTTATCATT	137	0.15634093736091934	No Hit
GTACATGGGAGTGGTATCAACGCAA	136	0.15519976263565716	No Hit
GTTATAGATTAACCCAATTTTAAGT	135	0.15405858791039498	No Hit
ATCTATAACTTTATAGATGCAACAC	134	0.15291741318513277	No Hit
GGTATCAACGCAGAGTACATGGAAG	133	0.1517762384598706	No Hit
AAAAAGTACTCTGCGTTGATACCAC	133	0.1517762384598706	No Hit
ATAAATAATCCACCTATAACTTCTC	132	0.15063506373460842	No Hit
CTCTAATACTTGTAATGCTAGAGGT	131	0.1494938890093462	No Hit
CTATAGAACTAGTACCGCAAGGGAA	131	0.1494938890093462	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	130	0.14835271428408403	No Hit
GTTCTACAAATCCCGTTTCCAACGA	129	0.14721153955882185	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	129	0.14721153955882185	No Hit
GTTATATAATTTAAGCTCCATAGGG	128	0.14607036483355967	No Hit
GATTAAACCTTGTACCTTTTGCATA	128	0.14607036483355967	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	123	0.14036449120724873	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	123	0.14036449120724873	No Hit
GAATATGGCAAGAAAACTGAAAATC	123	0.14036449120724873	No Hit
ATCGTAAATAGATAGAAACCGACCT	120	0.1369409670314622	No Hit
CTGTTAACCCAACACCGGAATGCCT	118	0.13465861758093783	No Hit
GTACTCTGCGTTGATACCACTGCTT	118	0.13465861758093783	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	117	0.13351744285567563	No Hit
CCTATAACTTCTCTGTTAACCCAAC	117	0.13351744285567563	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	117	0.13351744285567563	No Hit
GTGGTATCAACGCAGAGTACATGGA	115	0.13123509340515127	No Hit
CTTCTACACCATTGGGATGTCCTGA	113	0.12895274395462689	No Hit
GATTAAAGATAAGAGACAGTTGGAC	112	0.1278115692293647	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	112	0.1278115692293647	No Hit
CTGTTAGTATGAGTAACAAGAATTC	109	0.12438804505357816	No Hit
CTGTAGAACATATTAGATGAGTGAG	106	0.12096452087779161	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	106	0.12096452087779161	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	106	0.12096452087779161	No Hit
CCTCTAGCATTACAAGTATTAGAGG	103	0.11754099670200505	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	103	0.11754099670200505	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	102	0.11639982197674285	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	100	0.1141174725262185	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	100	0.1141174725262185	No Hit
ACATGGAAGCAGTGGTATCAACGCA	99	0.11297629780095629	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	97	0.11069394835043195	No Hit
GATATACACTGTTCTACAATGCCGG	96	0.10955277362516974	No Hit
GTTTAAAATTGAACTTAAATTCATT	96	0.10955277362516974	No Hit
GGTATCAACGCAGAGTACATGGGAG	95	0.10841159889990756	No Hit
GGAATATGGCGAGAAAACTGAAAAT	95	0.10841159889990756	No Hit
AAAGTACTCTGCGTTGATACCACTG	94	0.10727042417464538	No Hit
GTACATGGGAGAAATCGTAAATAGA	94	0.10727042417464538	No Hit
GTATCAACGCAGAGTACATGGGAGT	93	0.10612924944938319	No Hit
AATCTAAACTTACTTTTTGATTTTG	92	0.10498807472412101	No Hit
TACTAACAGTGTTGCATCTATAAAG	90	0.10270572527359663	No Hit
GATAGAAACCGACCTGGATTGCTCC	89	0.10156455054833445	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	89	0.10156455054833445	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCATTA	25	0.0059845457	19.001715	6
GAAATGT	50	4.4600056E-6	17.101543	6
TTTAGAA	50	4.4844146E-6	17.09177	2
ATTTAGA	50	4.5089437E-6	17.08201	1
GAACAGT	45	3.4749915E-5	16.890413	6
AACTCAG	35	0.0021469605	16.287186	17
ACTCAGA	35	0.0021469605	16.287186	18
TCCGGTC	35	0.0021469605	16.287186	8
TGCTCCG	35	0.0021469605	16.287186	5
CTCCGGT	35	0.0021469605	16.287186	7
CCGGTCT	35	0.0021469605	16.287186	9
TTAGAAA	55	1.1032815E-5	15.546859	3
AGTGTAT	55	1.1032815E-5	15.546859	10
AATGTCC	55	1.1032815E-5	15.546859	8
GGTCTGA	40	0.005223447	14.251287	11
CTGAACT	40	0.005223447	14.251287	14
GAACTCA	40	0.005223447	14.251287	16
TCTGAAC	40	0.005223447	14.251287	13
GCTCCGG	40	0.005223447	14.251287	6
TGAACTC	40	0.005223447	14.251287	15
>>END_MODULE