FastQCFastQC Report
Thu 2 Feb 2017
SRR4062235_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062235_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115090
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51970.1668330610030529No Hit
GCGCAAGACGGACCAGAGCGAAAGC47670.15302928647326403No Hit
GAATAGGACCGCGGTTCTATTTTGT40380.12962707337508708No Hit
GGGTAGGCACACGCTGAGCCAGTCA39460.12667370766173688No Hit
GATTAAGAGGGACGGCCGGGGGCAT39260.12603167163709555No Hit
ATCAGATACCGTCGTAGTTCCGACC37650.12086328163873276No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG36770.11803832313031085No Hit
TCGTAGTTCCGACCATAAACGATGC36520.11723577809950916No Hit
GAACTACGACGGTATCTGATCGTCT34080.1094029385988848No Hit
GTCTTGCGCCGGTCCAAGAATTTCA32880.10555072245103674No Hit
GTATCTGATCGTCTTCGAACCTCCG32780.10522970443871606No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC32430.10410614139559371No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT31910.10243684773152621No Hit
GAATAACGCCGCCGCATCGCCAGTC31490.1010885720797794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35400.012.7697241
CGGACCA9150.012.1489299
CGGACAT4900.012.02100855
TAGGACG951.040853E-612.000024
AAGACGG9200.011.9788595
CAAGACG9550.011.9371924
CGCATCG5950.011.81533513
GGTATCA14300.011.7547931
CGTCGTA7600.011.626327510
ACGGTAT5900.011.5945439
CGTTATT4300.011.486362
GCGTTAT4150.011.441941
CGAACGA2350.011.31898716
CGCAAGA9750.011.3005852
CGGTCCA6650.011.1441310
ATCGCCA6250.010.94384316
GCATCGC6450.010.89924314
TATTCCG700.00149150910.8576855
CCGTCGT7900.010.824029
ACACCGT1158.814259E-710.7401856
Produced by FastQC (version 0.11.5)