##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062235_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3115090 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23718576349319 32.0 32.0 32.0 32.0 32.0 2 30.73598098289295 32.0 32.0 32.0 32.0 32.0 3 30.740157427233243 32.0 32.0 32.0 32.0 32.0 4 30.744863551293864 32.0 32.0 32.0 32.0 32.0 5 30.631400376875145 32.0 32.0 32.0 32.0 32.0 6 34.320902124818225 36.0 36.0 36.0 32.0 36.0 7 34.238910272255374 36.0 36.0 36.0 32.0 36.0 8 34.1872093583171 36.0 36.0 36.0 32.0 36.0 9 34.34915010481238 36.0 36.0 36.0 32.0 36.0 10 33.96884648597633 36.0 36.0 36.0 32.0 36.0 11 34.34790198678048 36.0 36.0 36.0 32.0 36.0 12 34.11626469861224 36.0 36.0 36.0 32.0 36.0 13 34.23863034454863 36.0 36.0 36.0 32.0 36.0 14 34.1105393423625 36.0 36.0 36.0 32.0 36.0 15 34.051113450975734 36.0 36.0 36.0 32.0 36.0 16 34.0597003617873 36.0 36.0 36.0 32.0 36.0 17 33.98210581395722 36.0 36.0 36.0 32.0 36.0 18 33.996947760738855 36.0 36.0 36.0 32.0 36.0 19 33.994206908949664 36.0 36.0 36.0 32.0 36.0 20 33.99176492492994 36.0 36.0 36.0 32.0 36.0 21 33.97229582451872 36.0 36.0 36.0 32.0 36.0 22 33.939940740074924 36.0 36.0 36.0 32.0 36.0 23 33.904408219345186 36.0 36.0 36.0 32.0 36.0 24 33.873998825074075 36.0 36.0 36.0 32.0 36.0 25 33.50845047815633 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 43.0 5 267.0 6 605.0 7 158.0 8 523.0 9 421.0 10 244.0 11 60.0 12 137.0 13 151.0 14 425.0 15 623.0 16 1006.0 17 1446.0 18 1982.0 19 2810.0 20 4386.0 21 6477.0 22 9923.0 23 14910.0 24 21098.0 25 29401.0 26 39754.0 27 50802.0 28 66449.0 29 86255.0 30 112328.0 31 151907.0 32 215989.0 33 304150.0 34 653410.0 35 1336947.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.848123038361045 16.875399871784214 11.267387701236027 26.009089388618712 2 17.28220958549406 19.18116441236539 36.44472280344466 27.091903198695892 3 19.413449876760076 22.842530903076728 27.627122670431596 30.116896549731592 4 12.99936014352305 15.170698490189135 34.634246415696104 37.195694950591715 5 15.487547939781566 35.93247792353591 32.63463199042537 15.945342146257158 6 35.732156985060406 34.38165426063806 16.11085538477748 13.775333369524054 7 31.401131831722783 29.703802016215853 19.74377926389161 19.151286888169754 8 28.841841943817688 32.300540124324684 18.717250523911275 20.140367407946353 9 27.32091381577319 14.203380929377934 18.250024988807265 40.22568026604161 10 16.68620534805908 26.16789293724081 30.03166584322402 27.114235871476083 11 37.853349080956455 20.999320417624908 21.506885513193993 19.640444988224644 12 25.159171482179254 23.227480453738774 27.762907379185737 23.850440684896235 13 29.359122791566573 19.00591486984522 24.879031558186675 26.75593078040154 14 24.131218415392784 19.23021297875869 24.221561158598277 32.41700744725025 15 25.590198476668665 26.690282213282458 21.533296152776728 26.18622315727215 16 26.54819160164712 25.42794625650678 22.71740569755079 25.306456444295318 17 24.589442598460174 25.618608292851313 24.286831886008812 25.5051172226797 18 25.437727090712876 24.686708818045265 25.141902571054125 24.733661520187734 19 26.08736167584704 24.714868605309515 24.526023481446778 24.67174623739667 20 26.22730852438294 24.009032731119127 23.944641748041683 25.819016996456245 21 27.300193719802575 23.90217680137085 23.79620766737131 25.00142181145527 22 26.691607429142323 23.663978390099704 24.307281676476837 25.337132504281136 23 24.813318108769952 23.832535371728447 25.079341008586624 26.274805510914973 24 25.164911148796925 24.651429401320318 24.670129506259393 25.513529943623357 25 25.37769825202203 23.994462042855872 24.564901188463082 26.062938516659013 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 450.0 1 450.0 2 674.5 3 899.0 4 899.0 5 899.0 6 2038.0 7 3177.0 8 3177.0 9 3177.0 10 3722.0 11 4267.0 12 4267.0 13 4267.0 14 5683.5 15 7100.0 16 7100.0 17 7100.0 18 11713.5 19 16327.0 20 16327.0 21 16327.0 22 26806.5 23 37286.0 24 37286.0 25 37286.0 26 54856.0 27 72426.0 28 72426.0 29 72426.0 30 90644.0 31 108862.0 32 108862.0 33 108862.0 34 139471.0 35 170080.0 36 170080.0 37 170080.0 38 200264.5 39 230449.0 40 230449.0 41 230449.0 42 270221.5 43 309994.0 44 309994.0 45 309994.0 46 350531.5 47 391069.0 48 391069.0 49 391069.0 50 409676.0 51 428283.0 52 428283.0 53 428283.0 54 406022.5 55 383762.0 56 383762.0 57 383762.0 58 359775.0 59 335788.0 60 335788.0 61 335788.0 62 300104.0 63 264420.0 64 264420.0 65 264420.0 66 217589.0 67 170758.0 68 170758.0 69 170758.0 70 129758.0 71 88758.0 72 88758.0 73 88758.0 74 66687.5 75 44617.0 76 44617.0 77 44617.0 78 36036.0 79 27455.0 80 27455.0 81 27455.0 82 18925.5 83 10396.0 84 10396.0 85 10396.0 86 7766.0 87 5136.0 88 5136.0 89 5136.0 90 3430.5 91 1725.0 92 1725.0 93 1725.0 94 1078.0 95 431.0 96 431.0 97 431.0 98 803.0 99 1175.0 100 1175.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.309522357299468E-4 2 2.5681440985653707E-4 3 8.988504344978797E-4 4 0.010561492605350086 5 0.032968549865332944 6 0.06269481780622711 7 0.10657798009046289 8 0.10911402238779618 9 0.11868035915495218 10 0.13376820573402373 11 0.13983544616688443 12 0.13280515169706172 13 0.12673791126420103 14 0.11575909524283408 15 0.13306196610691826 16 0.1193544969808256 17 0.12301410232128124 18 0.11068701064816747 19 0.09681903251591446 20 0.09158643891508753 21 0.09190745692740819 22 0.10218003332166968 23 0.08269423997380493 24 0.0901418578596445 25 0.08831205518941668 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3115090.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.85604723135368 #Duplication Level Percentage of deduplicated Percentage of total 1 65.24637899977944 23.39477247090894 2 15.987851604051375 11.465223244854796 3 6.885701934539033 7.40682161357565 4 3.636300151055317 5.215333998544718 5 2.1444107537367683 3.8445046634704148 6 1.3415169733998125 2.886089757593179 7 0.9150383175752871 2.296675999343453 8 0.6440878255587928 1.8475554795500775 9 0.4811739375817957 1.5527695889186364 >10 2.4032436913540387 15.328240511597452 >50 0.1600965419359873 3.963357660604162 >100 0.12592260906593042 9.1602394585343 >500 0.017020028054921692 4.253749828150856 >1k 0.011166579252920118 7.216857363388146 >5k 9.005305849129128E-5 0.1678083609652052 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5197 0.1668330610030529 No Hit GCGCAAGACGGACCAGAGCGAAAGC 4767 0.15302928647326403 No Hit GAATAGGACCGCGGTTCTATTTTGT 4038 0.12962707337508708 No Hit GGGTAGGCACACGCTGAGCCAGTCA 3946 0.12667370766173688 No Hit GATTAAGAGGGACGGCCGGGGGCAT 3926 0.12603167163709555 No Hit ATCAGATACCGTCGTAGTTCCGACC 3765 0.12086328163873276 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 3677 0.11803832313031085 No Hit TCGTAGTTCCGACCATAAACGATGC 3652 0.11723577809950916 No Hit GAACTACGACGGTATCTGATCGTCT 3408 0.1094029385988848 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 3288 0.10555072245103674 No Hit GTATCTGATCGTCTTCGAACCTCCG 3278 0.10522970443871606 No Hit GTGTAGCGCGCGTGCAGCCCCGGAC 3243 0.10410614139559371 No Hit CTCCTGGTGGTGCCCTTCCGTCAAT 3191 0.10243684773152621 No Hit GAATAACGCCGCCGCATCGCCAGTC 3149 0.1010885720797794 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.420360246413427E-5 2 0.0 0.0 0.0 0.0 6.420360246413427E-5 3 0.0 0.0 0.0 0.0 6.420360246413427E-5 4 0.0 0.0 0.0 0.0 6.420360246413427E-5 5 0.0 0.0 0.0 0.0 6.420360246413427E-5 6 0.0 0.0 0.0 0.0 9.63054036962014E-5 7 0.0 0.0 0.0 6.420360246413427E-5 9.63054036962014E-5 8 0.0 0.0 0.0 6.420360246413427E-5 9.63054036962014E-5 9 0.0 0.0 0.0 6.420360246413427E-5 9.63054036962014E-5 10 0.0 0.0 0.0 6.420360246413427E-5 9.63054036962014E-5 11 0.0 0.0 0.0 6.420360246413427E-5 9.63054036962014E-5 12 0.0 0.0 0.0 6.420360246413427E-5 1.2840720492826853E-4 13 0.0 0.0 0.0 6.420360246413427E-5 1.6050900616033565E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3540 0.0 12.769724 1 CGGACCA 915 0.0 12.148929 9 CGGACAT 490 0.0 12.0210085 5 TAGGACG 95 1.040853E-6 12.00002 4 AAGACGG 920 0.0 11.978859 5 CAAGACG 955 0.0 11.937192 4 CGCATCG 595 0.0 11.815335 13 GGTATCA 1430 0.0 11.754793 1 CGTCGTA 760 0.0 11.6263275 10 ACGGTAT 590 0.0 11.594543 9 CGTTATT 430 0.0 11.48636 2 GCGTTAT 415 0.0 11.44194 1 CGAACGA 235 0.0 11.318987 16 CGCAAGA 975 0.0 11.300585 2 CGGTCCA 665 0.0 11.14413 10 ATCGCCA 625 0.0 10.943843 16 GCATCGC 645 0.0 10.899243 14 TATTCCG 70 0.001491509 10.857685 5 CCGTCGT 790 0.0 10.82402 9 ACACCGT 115 8.814259E-7 10.740185 6 >>END_MODULE