FastQCFastQC Report
Thu 2 Feb 2017
SRR4062235_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062235_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115090
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT47150.15135999280919654No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT42940.13784513449049626No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA41270.13248413368474105No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA39460.12667370766173688No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT38720.12429817437056394No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC38680.12416976716563566No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG36230.11630482586377923No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA33020.10600014766828567No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC32730.10506919543255572No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA32640.10478027922146713No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC31760.10195532071304522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40900.028.5155161
GGTATCA19000.021.6579171
ACCGTCG9800.019.530058
TCAACGC60550.019.1112354
CGTCGTA10800.018.94389710
ATACCGT10800.018.74026
ATCAACG62250.018.6603013
AACGCAG62450.018.5999456
CAACGCA62400.018.5798915
TACCGTC10850.018.2483227
TATCAAC65900.017.8941252
CGCAATA10550.017.51692836
GGCGTTA11400.017.17659842
CCGTCGT11150.017.1654249
AACGTAC800.001988614716.50084943
ATACGAA11100.016.45182440
CTAGCGG11200.016.303129
TAATACT6350.016.2832574
GTCGTAG11800.016.21987211
TACGAAT11550.016.00133741
Produced by FastQC (version 0.11.5)