##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062235_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3115090
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24244211242693	32.0	32.0	32.0	32.0	32.0
2	31.475043417686166	32.0	32.0	32.0	32.0	32.0
3	31.581239386341966	32.0	32.0	32.0	32.0	32.0
4	31.628472050566756	32.0	32.0	32.0	32.0	32.0
5	31.63856646196418	32.0	32.0	32.0	32.0	32.0
6	35.245393552032205	36.0	36.0	36.0	36.0	36.0
7	35.25428928217162	36.0	36.0	36.0	36.0	36.0
8	35.20013482756517	36.0	36.0	36.0	36.0	36.0
9	35.31388980735709	36.0	36.0	36.0	36.0	36.0
10	35.187413846790946	36.0	36.0	36.0	36.0	36.0
11	35.308052736839066	36.0	36.0	36.0	36.0	36.0
12	35.233166938996945	36.0	36.0	36.0	36.0	36.0
13	35.26004256698843	36.0	36.0	36.0	36.0	36.0
14	35.20147154656848	36.0	36.0	36.0	36.0	36.0
15	35.17705234840727	36.0	36.0	36.0	36.0	36.0
16	35.21507500585858	36.0	36.0	36.0	36.0	36.0
17	35.19938589254243	36.0	36.0	36.0	36.0	36.0
18	35.183309952521434	36.0	36.0	36.0	36.0	36.0
19	35.186339720521715	36.0	36.0	36.0	36.0	36.0
20	35.18068627230674	36.0	36.0	36.0	36.0	36.0
21	35.17684753891541	36.0	36.0	36.0	36.0	36.0
22	35.16295066916204	36.0	36.0	36.0	36.0	36.0
23	35.107744559547235	36.0	36.0	36.0	36.0	36.0
24	35.11821456201908	36.0	36.0	36.0	36.0	36.0
25	35.09491186450472	36.0	36.0	36.0	36.0	36.0
26	35.06060210138391	36.0	36.0	36.0	36.0	36.0
27	35.04791450648296	36.0	36.0	36.0	36.0	36.0
28	35.02152522078014	36.0	36.0	36.0	36.0	36.0
29	34.98291863156442	36.0	36.0	36.0	36.0	36.0
30	34.98866934823713	36.0	36.0	36.0	36.0	36.0
31	34.98143938056364	36.0	36.0	36.0	36.0	36.0
32	34.92328311541561	36.0	36.0	36.0	32.0	36.0
33	34.88936531528784	36.0	36.0	36.0	32.0	36.0
34	34.87266050098071	36.0	36.0	36.0	32.0	36.0
35	34.85718390158872	36.0	36.0	36.0	32.0	36.0
36	34.84070187378214	36.0	36.0	36.0	32.0	36.0
37	34.84578712011531	36.0	36.0	36.0	32.0	36.0
38	34.83678609606785	36.0	36.0	36.0	32.0	36.0
39	34.83564905026821	36.0	36.0	36.0	32.0	36.0
40	34.83530042470683	36.0	36.0	36.0	32.0	36.0
41	34.82296659165546	36.0	36.0	36.0	32.0	36.0
42	34.79129559659592	36.0	36.0	36.0	32.0	36.0
43	34.80267793225878	36.0	36.0	36.0	32.0	36.0
44	34.748814319971494	36.0	36.0	36.0	32.0	36.0
45	34.734599963403944	36.0	36.0	36.0	32.0	36.0
46	34.71555300167893	36.0	36.0	36.0	32.0	36.0
47	34.699592949160376	36.0	36.0	36.0	32.0	36.0
48	34.670258644212524	36.0	36.0	36.0	32.0	36.0
49	34.6581058653201	36.0	36.0	36.0	32.0	36.0
50	34.248035530273604	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	16.0
20	28.0
21	97.0
22	314.0
23	734.0
24	1772.0
25	3966.0
26	8078.0
27	14717.0
28	25618.0
29	39465.0
30	58401.0
31	84335.0
32	126694.0
33	213844.0
34	496310.0
35	2040697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.331609445471145	17.428136996902783	11.764643546980896	26.475610010645184
2	16.57385612857743	19.61509469821005	36.28596689254523	27.525082280667284
3	18.766381997771486	23.175191913147682	27.96094226763285	30.09748382144798
4	12.610903697806483	15.608184675242128	34.72387635670238	37.05703527024901
5	15.070200264005381	36.320579065503125	32.544152845762305	16.06506782472919
6	35.82360165979367	34.45734724498778	16.0837935132449	13.635257581973658
7	31.38586686098957	29.70880456102392	19.832364393966145	19.072964184020368
8	28.927350413631707	32.294572548465695	18.666812194832254	20.111264843070344
9	27.410765018025163	13.906757108141338	18.14718033828878	40.535297535544714
10	16.56289756087226	26.169268357982713	30.21550909139354	27.052324989751497
11	38.13835234294996	20.81699084135611	21.54101486634415	19.503641949349777
12	25.151632858119672	23.112943767274782	27.839645082485575	23.89577829211997
13	29.470159770664733	18.8921026358789	25.016163256920347	26.621574336536025
14	24.226555252015192	19.055693414957513	24.336824939247343	32.38092639377996
15	25.637782535978094	26.576021880587717	21.60078842023826	26.18540716319593
16	26.643093092611082	25.23888708760959	22.842504341796495	25.27551547798284
17	24.704390563354508	25.49149462776356	24.3792314186749	25.424883390207025
18	25.56987227310724	24.44970986336163	25.281437033479676	24.698980830051454
19	26.181394438041917	24.561280733461956	24.606897393012723	24.650427435483405
20	26.350277023116238	23.833072826630943	24.083948967009867	25.732701183242952
21	27.42167963044406	23.783421987807735	23.89298543541278	24.90191294633542
22	26.709790086321743	23.59135691103628	24.44102738604663	25.257825616595348
23	24.907731368188838	23.602535914704205	25.259021491841803	26.230711225265154
24	25.371805687708903	24.348007812349522	24.76613672638928	25.514049773552294
25	25.505676085451224	23.75760291767132	24.789034784582263	25.947686212295196
26	25.15925775165678	24.948733193026428	25.081763655204558	24.81024540011223
27	25.902899319310347	24.19591046895806	24.388971569232982	25.512218642498606
28	25.310333890630982	23.92874294710295	25.202405625367575	25.558517536898496
29	25.011299899328172	24.382678795225278	25.181215715078174	25.424805590368376
30	25.151650564227417	24.41487408252051	25.25315756763281	25.18031778561926
31	25.66940538888593	24.320675116321326	24.165430207526104	25.844489287266644
32	25.798241770235936	24.12774693304955	23.99776569670296	26.076245600011553
33	25.15247597092981	23.998798105089403	24.729533517748013	26.119192406232777
34	25.934598265520194	24.127824388333433	24.89801945712034	25.03955788902604
35	26.467112733184145	23.683878525149105	25.07626607462214	24.77274266704461
36	24.950530965496874	24.633877781630005	24.538053597339392	25.87753765553373
37	26.19891147255536	24.33254340081039	24.406282563850027	25.062262562784227
38	25.33734177824718	23.997605205628087	24.822942515304533	25.842110500820205
39	26.189413764853626	24.003551772933076	24.142971059342603	25.664063402870696
40	26.348028161011232	24.137817017630645	24.6350101939368	24.879144627421322
41	24.88362171221907	24.8861898835238	24.97973552329861	25.25045288095852
42	26.52362416430572	24.534972752152942	24.471664646821353	24.469738436719986
43	25.883630645776947	23.61991049005108	24.712412129718476	25.784046734453497
44	25.204576871786085	24.491374865728872	24.391341671145046	25.91270659134
45	25.519804046201948	24.616567469871782	24.46257166888174	25.401056815044527
46	25.228089008966897	24.57155863283994	24.41014673458722	25.790205623605942
47	25.50847974523847	24.26073892400492	24.928878002741513	25.3019033280151
48	26.458435327523745	24.891836665901543	23.55169319719483	25.098034809379882
49	25.363284486624803	25.117568859926365	23.901189829928445	25.617956823520384
50	25.604711335970006	24.933484000205457	24.095356720940984	25.366447942883557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	196.0
1	145.0
2	94.0
3	257.0
4	420.0
5	443.0
6	466.0
7	377.5
8	289.0
9	408.0
10	527.0
11	907.5
12	1288.0
13	2342.5
14	3397.0
15	4444.0
16	5491.0
17	5376.0
18	5261.0
19	5389.5
20	5518.0
21	6221.0
22	6924.0
23	8103.0
24	9282.0
25	12064.0
26	14846.0
27	18957.5
28	23069.0
29	26630.5
30	30192.0
31	33959.5
32	37727.0
33	43189.0
34	48651.0
35	57423.5
36	66196.0
37	81759.0
38	97322.0
39	109206.0
40	121090.0
41	133866.0
42	146642.0
43	152818.5
44	158995.0
45	176618.5
46	194242.0
47	209299.5
48	224357.0
49	237117.5
50	249878.0
51	242853.0
52	235828.0
53	237281.5
54	238735.0
55	244125.5
56	249516.0
57	244911.0
58	240306.0
59	220666.0
60	201026.0
61	176291.0
62	151556.0
63	130385.5
64	109215.0
65	91584.0
66	73953.0
67	63794.0
68	53635.0
69	47951.5
70	42268.0
71	32649.5
72	23031.0
73	20423.0
74	17815.0
75	12957.0
76	8099.0
77	7354.0
78	6609.0
79	5641.0
80	4673.0
81	3653.0
82	2633.0
83	2299.5
84	1966.0
85	1581.0
86	1196.0
87	778.5
88	361.0
89	243.5
90	126.0
91	87.5
92	49.0
93	44.5
94	40.0
95	43.5
96	47.0
97	38.0
98	29.0
99	23.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03226231023822747
2	0.004558455774953533
3	2.889162110886042E-4
4	0.0
5	6.420360246413427E-5
6	1.2840720492826853E-4
7	0.0
8	0.0
9	0.0
10	3.210180123206713E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.420360246413426E-4
17	0.0
18	1.926108073924028E-4
19	0.0
20	2.2471260862446993E-4
21	0.0
22	0.0
23	3.210180123206713E-5
24	7.062396271054769E-4
25	9.63054036962014E-5
26	4.4942521724893985E-4
27	4.4942521724893985E-4
28	0.0018619044714598936
29	5.778324221772084E-4
30	0.0010914612418902825
31	3.852216147848056E-4
32	8.025450308016784E-4
33	2.889162110886042E-4
34	0.0011235630431223496
35	2.889162110886042E-4
36	3.210180123206713E-4
37	0.0017977008689957594
38	0.0
39	8.346468320337454E-4
40	9.63054036962014E-5
41	0.0010593594406582154
42	0.005296797203291077
43	0.0041411323589366595
44	0.003980623352776324
45	0.002247126086244699
46	0.002503940496101236
47	6.420360246413426E-4
48	0.004012725154008391
49	0.003434892731831183
50	0.0016050900616033567
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3115090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.037441259008055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.191965224814574	22.772767348305354
2	17.02587316902075	12.271378084238133
3	7.453959854723023	8.058649212347555
4	3.8716658259451266	5.5809971910802565
5	2.254116902172103	4.061630272648219
6	1.4118400291199216	3.0527461269915244
7	0.9335291605270808	2.3549401600246043
8	0.6740180772797149	1.943190949398182
9	0.4780133416049737	1.5503739947200252
>10	2.404276596044058	15.321099552263433
>50	0.15815934227077552	3.9493885029000664
>100	0.11664145280414785	8.474576816983777
>500	0.015259425690442918	3.7524285846984218
>1k	0.010681597983238407	6.855833203400459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	4715	0.15135999280919654	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	4294	0.13784513449049626	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	4127	0.13248413368474105	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3946	0.12667370766173688	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	3872	0.12429817437056394	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3868	0.12416976716563566	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	3623	0.11630482586377923	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	3302	0.10600014766828567	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3273	0.10506919543255572	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	3264	0.10478027922146713	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3176	0.10195532071304522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.63054036962014E-5
2	0.0	0.0	0.0	0.0	9.63054036962014E-5
3	0.0	0.0	0.0	0.0	9.63054036962014E-5
4	0.0	0.0	0.0	0.0	9.63054036962014E-5
5	0.0	0.0	0.0	0.0	9.63054036962014E-5
6	0.0	0.0	0.0	0.0	9.63054036962014E-5
7	0.0	0.0	0.0	0.0	9.63054036962014E-5
8	0.0	0.0	0.0	0.0	9.63054036962014E-5
9	0.0	0.0	0.0	3.210180123206713E-5	1.2840720492826853E-4
10	0.0	0.0	0.0	3.210180123206713E-5	1.2840720492826853E-4
11	0.0	0.0	0.0	3.210180123206713E-5	1.2840720492826853E-4
12	0.0	0.0	0.0	3.210180123206713E-5	2.2471260862446993E-4
13	0.0	0.0	0.0	3.210180123206713E-5	2.889162110886042E-4
14	0.0	0.0	0.0	3.210180123206713E-5	3.210180123206713E-4
15	0.0	0.0	0.0	3.210180123206713E-5	3.852216147848056E-4
16	0.0	0.0	0.0	1.6050900616033565E-4	4.173234160168727E-4
17	0.0	0.0	0.0	2.2471260862446993E-4	4.4942521724893985E-4
18	0.0	0.0	0.0	3.210180123206713E-4	5.457306209451412E-4
19	0.0	0.0	0.0	4.8152701848100697E-4	5.457306209451412E-4
20	0.0	0.0	0.0	8.988504344978797E-4	5.457306209451412E-4
21	0.0	0.0	0.0	0.0010914612418902825	5.457306209451412E-4
22	0.0	0.0	0.0	0.0013482756517468196	6.099342234092755E-4
23	0.0	0.0	0.0	0.0017655990677636923	6.741378258734098E-4
24	0.0	0.0	0.0	0.0023755332911729677	7.062396271054769E-4
25	0.0	0.0	0.0	0.003113874719510512	7.062396271054769E-4
26	0.0	0.0	0.0	0.0037880125453839215	7.062396271054769E-4
27	0.0	0.0	0.0	0.004397946768793197	7.062396271054769E-4
28	0.0	0.0	0.0	0.005361000805755211	7.062396271054769E-4
29	0.0	0.0	0.0	0.006580869252573762	7.38341428337544E-4
30	0.0	0.0	0.0	0.008218061115409186	7.38341428337544E-4
31	0.0	0.0	0.0	0.012230786269417577	7.38341428337544E-4
32	0.0	0.0	0.0	0.016853445646835243	9.309522357299468E-4
33	0.0	0.0	0.0	0.021989733843965985	9.309522357299468E-4
34	0.0	0.0	0.0	0.027543345457113597	9.951558381940812E-4
35	0.0	0.0	0.0	0.03277593905794054	9.951558381940812E-4
36	0.0	0.0	0.0	0.03993464073269151	0.0010272576394261483
37	0.0	0.0	0.0	0.05168389998362808	0.0010272576394261483
38	0.0	0.0	0.0	0.06648283035161102	0.0011235630431223496
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4090	0.0	28.515516	1
GGTATCA	1900	0.0	21.657917	1
ACCGTCG	980	0.0	19.53005	8
TCAACGC	6055	0.0	19.111235	4
CGTCGTA	1080	0.0	18.943897	10
ATACCGT	1080	0.0	18.7402	6
ATCAACG	6225	0.0	18.660301	3
AACGCAG	6245	0.0	18.599945	6
CAACGCA	6240	0.0	18.579891	5
TACCGTC	1085	0.0	18.248322	7
TATCAAC	6590	0.0	17.894125	2
CGCAATA	1055	0.0	17.516928	36
GGCGTTA	1140	0.0	17.176598	42
CCGTCGT	1115	0.0	17.165424	9
AACGTAC	80	0.0019886147	16.500849	43
ATACGAA	1110	0.0	16.451824	40
CTAGCGG	1120	0.0	16.3031	29
TAATACT	635	0.0	16.283257	4
GTCGTAG	1180	0.0	16.219872	11
TACGAAT	1155	0.0	16.001337	41
>>END_MODULE