FastQCFastQC Report
Thu 2 Feb 2017
SRR4062234_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062234_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641125
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31270.19054002589686952No Hit
TATCAACGCAGAGTACTTTTTTTTT22470.13691827252646813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7100.014.3118221
TCGCGTA1203.6379788E-1214.2514649
CGACTTA400.00527673614.2510312
GTATCAA20000.012.8679331
CGCGTAA1353.7107384E-1011.96419310
GCGTAAC1353.7107384E-1011.96382711
CGCAATA1052.7250098E-711.7602432
AAGACGG2850.011.6667985
ATCGCCA2700.011.61124216
CGCATCG2550.011.54950113
CAAGACG2950.011.2713144
TAGGACG1701.0913936E-1111.1765964
GCACCGT600.005874065711.08379656
CGGTTCT2700.010.908195512
ACGGTAT2450.010.8582589
CGGACAT1752.0008883E-1110.8572655
CGCCAGT2800.010.85726518
CATCGCC2900.010.81079715
GGGTAGG4400.010.7916251
GCATCGC2650.010.75516514
Produced by FastQC (version 0.11.5)