##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062234_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1641125
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25628760758626	32.0	32.0	32.0	32.0	32.0
2	30.78198370020565	32.0	32.0	32.0	32.0	32.0
3	30.809041968162084	32.0	32.0	32.0	32.0	32.0
4	30.813948358595475	32.0	32.0	32.0	32.0	32.0
5	30.737974255465	32.0	32.0	32.0	32.0	32.0
6	34.403370858405054	36.0	36.0	36.0	32.0	36.0
7	34.32772945388072	36.0	36.0	36.0	32.0	36.0
8	34.29030695407114	36.0	36.0	36.0	32.0	36.0
9	34.42040977987661	36.0	36.0	36.0	32.0	36.0
10	34.10014654581461	36.0	36.0	36.0	32.0	36.0
11	34.40793845685125	36.0	36.0	36.0	32.0	36.0
12	34.22211348922233	36.0	36.0	36.0	32.0	36.0
13	34.31347155152715	36.0	36.0	36.0	32.0	36.0
14	34.216653819788256	36.0	36.0	36.0	32.0	36.0
15	34.17036118516262	36.0	36.0	36.0	32.0	36.0
16	34.16602452585879	36.0	36.0	36.0	32.0	36.0
17	34.100086830680176	36.0	36.0	36.0	32.0	36.0
18	34.10399451595704	36.0	36.0	36.0	32.0	36.0
19	34.11617518470561	36.0	36.0	36.0	32.0	36.0
20	34.10556234290502	36.0	36.0	36.0	32.0	36.0
21	34.090951024449694	36.0	36.0	36.0	32.0	36.0
22	34.04720420443294	36.0	36.0	36.0	32.0	36.0
23	34.02493228730292	36.0	36.0	36.0	32.0	36.0
24	33.99589549851474	36.0	36.0	36.0	32.0	36.0
25	33.6367851321502	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	20.0
5	129.0
6	309.0
7	90.0
8	274.0
9	219.0
10	126.0
11	42.0
12	73.0
13	77.0
14	287.0
15	412.0
16	623.0
17	839.0
18	1069.0
19	1499.0
20	2204.0
21	3158.0
22	4669.0
23	7182.0
24	9996.0
25	13970.0
26	19219.0
27	24381.0
28	32423.0
29	42616.0
30	56261.0
31	76504.0
32	108741.0
33	155139.0
34	341370.0
35	737202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.662739229201804	17.722033460402265	12.06236777193889	25.55285953845704
2	16.69067466120698	19.443733547821	37.415179877157065	26.450411913814953
3	18.99531720811765	22.799864726116088	28.464854687209613	29.73996337855665
4	12.99387309120648	15.638974306472194	35.12631036260526	36.24084223971607
5	15.15319414018547	36.23703064370431	33.17302593322678	15.436749282883437
6	34.803323207946825	34.80801803790252	16.472877906374116	13.91578084777654
7	30.616956670786056	30.363571595008427	20.00894228505272	19.010529449152802
8	28.216848184798348	33.1276588736075	19.06245386812353	19.593039073470617
9	26.95106461637573	14.706176617361585	18.755879456948648	39.586879309314035
10	16.699360143407926	26.741758955826285	30.745148908363284	25.813731992402506
11	36.81364445982137	21.521778793143113	21.945742635397806	19.718834111637708
12	24.22932835456631	23.95366573924928	28.436447503294772	23.380558402889637
13	29.30075660004039	19.591676362365114	25.14638115046402	25.961185887130476
14	23.398384597552493	19.777455131098936	25.077841900416054	31.74631837093252
15	25.073048882500053	27.288158240749837	22.10624837169175	25.532544505058357
16	25.646179900583952	26.093356889941994	23.550853723709288	24.709609485764762
17	24.02135524415711	26.328190154649207	25.026035168678	24.624419432515683
18	24.58717185729048	25.518726872778778	25.81202243393835	24.082078835992395
19	25.378536283674862	25.30034357218706	25.287169137178196	24.03395100695988
20	25.602710359317776	24.72080676492024	24.92390074620266	24.752582129559322
21	26.440700294086433	24.44229087356235	24.637271034010837	24.47973779834038
22	25.775865802252042	24.431485137765318	25.094861758923958	24.697787301058682
23	24.459183468116937	24.552915039678886	25.51450217070611	25.473399321498068
24	24.514888779378687	25.116734080496506	25.35160500212244	25.01677213800237
25	24.79561623116635	24.75225424395539	25.316204026992988	25.135925497885264
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	423.0
1	423.0
2	514.0
3	605.0
4	605.0
5	605.0
6	1207.5
7	1810.0
8	1810.0
9	1810.0
10	2302.0
11	2794.0
12	2794.0
13	2794.0
14	3825.5
15	4857.0
16	4857.0
17	4857.0
18	8119.0
19	11381.0
20	11381.0
21	11381.0
22	17704.5
23	24028.0
24	24028.0
25	24028.0
26	34652.5
27	45277.0
28	45277.0
29	45277.0
30	56894.5
31	68512.0
32	68512.0
33	68512.0
34	84579.0
35	100646.0
36	100646.0
37	100646.0
38	117816.5
39	134987.0
40	134987.0
41	134987.0
42	154182.5
43	173378.0
44	173378.0
45	173378.0
46	192085.5
47	210793.0
48	210793.0
49	210793.0
50	216717.0
51	222641.0
52	222641.0
53	222641.0
54	209055.0
55	195469.0
56	195469.0
57	195469.0
58	179549.0
59	163629.0
60	163629.0
61	163629.0
62	142929.5
63	122230.0
64	122230.0
65	122230.0
66	99762.0
67	77294.0
68	77294.0
69	77294.0
70	58690.0
71	40086.0
72	40086.0
73	40086.0
74	30082.5
75	20079.0
76	20079.0
77	20079.0
78	15913.5
79	11748.0
80	11748.0
81	11748.0
82	8172.5
83	4597.0
84	4597.0
85	4597.0
86	3424.5
87	2252.0
88	2252.0
89	2252.0
90	1503.5
91	755.0
92	755.0
93	755.0
94	486.0
95	217.0
96	217.0
97	217.0
98	427.0
99	637.0
100	637.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012796100236118517
2	3.046690532409171E-4
3	6.093381064818342E-4
4	0.01017594637824663
5	0.032112118211592655
6	0.06233528829309163
7	0.10498895574682002
8	0.1098636605986747
9	0.11839439408942037
10	0.1318607662426689
11	0.14051336735471093
12	0.13192170005331708
13	0.12686419376951788
14	0.11607890928478941
15	0.13125142813618706
16	0.11900373219590221
17	0.12162388605377408
18	0.11108233681163837
19	0.09664102368801888
20	0.09079137786579328
21	0.0896336354634778
22	0.09956584659913169
23	0.08140757102597304
24	0.0914616497829233
25	0.08774468733338411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1641125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.4092971038736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40335171307554	43.442746090092996
2	12.754110649678488	14.133926125674273
3	3.7772275632894305	6.278805728497337
4	1.6001294820182317	3.546481994952623
5	0.8404118722134842	2.3283315558549798
6	0.5561447122467824	1.848935255617815
7	0.35182656117959427	1.3646123720204009
8	0.2816300584123808	1.2483938863962307
9	0.2012834760936269	1.003767833607499
>10	1.08595694612904	10.801405345763717
>50	0.07640968510940489	2.9169791526639144
>100	0.06322728245525104	7.11125681135481
>500	0.006300398555031094	2.4023024750428648
>1k	0.00198959954369403	1.572055372460745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3127	0.19054002589686952	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2247	0.13691827252646813	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2186762129636682E-4
2	0.0	0.0	0.0	0.0	1.2186762129636682E-4
3	0.0	0.0	0.0	0.0	1.2186762129636682E-4
4	0.0	0.0	0.0	0.0	1.2186762129636682E-4
5	0.0	0.0	0.0	0.0	1.2186762129636682E-4
6	0.0	0.0	0.0	0.0	1.2186762129636682E-4
7	0.0	0.0	0.0	0.0	1.2186762129636682E-4
8	0.0	0.0	0.0	0.0	1.2186762129636682E-4
9	0.0	0.0	0.0	1.2186762129636682E-4	1.2186762129636682E-4
10	0.0	0.0	0.0	1.2186762129636682E-4	1.2186762129636682E-4
11	0.0	0.0	0.0	1.8280143194455024E-4	1.2186762129636682E-4
12	0.0	0.0	0.0	1.8280143194455024E-4	3.046690532409171E-4
13	0.0	0.0	0.0	3.046690532409171E-4	3.656028638891005E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	710	0.0	14.311822	1
TCGCGTA	120	3.6379788E-12	14.251464	9
CGACTTA	40	0.005276736	14.25103	12
GTATCAA	2000	0.0	12.867933	1
CGCGTAA	135	3.7107384E-10	11.964193	10
GCGTAAC	135	3.7107384E-10	11.963827	11
CGCAATA	105	2.7250098E-7	11.760243	2
AAGACGG	285	0.0	11.666798	5
ATCGCCA	270	0.0	11.611242	16
CGCATCG	255	0.0	11.549501	13
CAAGACG	295	0.0	11.271314	4
TAGGACG	170	1.0913936E-11	11.176596	4
GCACCGT	60	0.0058740657	11.0837965	6
CGGTTCT	270	0.0	10.9081955	12
ACGGTAT	245	0.0	10.858258	9
CGGACAT	175	2.0008883E-11	10.857265	5
CGCCAGT	280	0.0	10.857265	18
CATCGCC	290	0.0	10.810797	15
GGGTAGG	440	0.0	10.791625	1
GCATCGC	265	0.0	10.755165	14
>>END_MODULE