FastQCFastQC Report
Thu 2 Feb 2017
SRR4062234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641125
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.027.2293721
GTATCAA21050.026.1406291
TAGGACG1551.8189894E-1221.2893664
TAACGGC856.1147857E-620.70621136
CGCAATA3350.019.04507836
GTATAGA2150.018.427321
CGAGCCG4400.017.99973915
TTAACGG1002.4835512E-517.6002835
ATCAACG31250.017.4584143
TCTATAC2150.017.3945673
TCAACGC31400.017.3750134
CAACGCA31700.017.2105835
ATACCGT4900.017.0604576
TATCAAC32750.016.8608232
TACACCG1053.777127E-516.761155
GACGGCC6450.016.71293411
AACGCAG32650.016.7098166
CAATACG3850.016.57219938
GCATTCG6400.016.4997622
CGGATCG800.001988951616.4997626