##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062234_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1641125
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.302240840886586	32.0	32.0	32.0	32.0	32.0
2	31.48750095209079	32.0	32.0	32.0	32.0	32.0
3	31.596082565313427	32.0	32.0	32.0	32.0	32.0
4	31.666690227740116	32.0	32.0	32.0	32.0	32.0
5	31.646279838525402	32.0	32.0	32.0	32.0	32.0
6	35.29431457079747	36.0	36.0	36.0	36.0	36.0
7	35.30256318074492	36.0	36.0	36.0	36.0	36.0
8	35.253085535836696	36.0	36.0	36.0	36.0	36.0
9	35.32555350750248	36.0	36.0	36.0	36.0	36.0
10	35.21613771041206	36.0	36.0	36.0	36.0	36.0
11	35.33073958412674	36.0	36.0	36.0	36.0	36.0
12	35.250331936933506	36.0	36.0	36.0	36.0	36.0
13	35.28460415873258	36.0	36.0	36.0	36.0	36.0
14	35.250829156828395	36.0	36.0	36.0	36.0	36.0
15	35.23333688780562	36.0	36.0	36.0	36.0	36.0
16	35.24151847056135	36.0	36.0	36.0	36.0	36.0
17	35.22932134968391	36.0	36.0	36.0	36.0	36.0
18	35.22143956127656	36.0	36.0	36.0	36.0	36.0
19	35.209453880722066	36.0	36.0	36.0	36.0	36.0
20	35.213875238022695	36.0	36.0	36.0	36.0	36.0
21	35.204374438266434	36.0	36.0	36.0	36.0	36.0
22	35.18796800974941	36.0	36.0	36.0	36.0	36.0
23	35.155897935867166	36.0	36.0	36.0	36.0	36.0
24	35.137015766623506	36.0	36.0	36.0	36.0	36.0
25	35.12950933048975	36.0	36.0	36.0	36.0	36.0
26	35.082155838220736	36.0	36.0	36.0	36.0	36.0
27	35.07456287607586	36.0	36.0	36.0	36.0	36.0
28	35.05358153705537	36.0	36.0	36.0	36.0	36.0
29	35.0312852464011	36.0	36.0	36.0	36.0	36.0
30	35.01243049737223	36.0	36.0	36.0	36.0	36.0
31	35.01875542691751	36.0	36.0	36.0	36.0	36.0
32	34.99488216924366	36.0	36.0	36.0	36.0	36.0
33	34.971710869068474	36.0	36.0	36.0	36.0	36.0
34	34.96414228044787	36.0	36.0	36.0	36.0	36.0
35	34.95032249219285	36.0	36.0	36.0	36.0	36.0
36	34.923414730748725	36.0	36.0	36.0	36.0	36.0
37	34.913530657323484	36.0	36.0	36.0	36.0	36.0
38	34.891422042806	36.0	36.0	36.0	32.0	36.0
39	34.882323406200015	36.0	36.0	36.0	32.0	36.0
40	34.86727641099855	36.0	36.0	36.0	32.0	36.0
41	34.840069464544136	36.0	36.0	36.0	32.0	36.0
42	34.80629507197806	36.0	36.0	36.0	32.0	36.0
43	34.80359936019499	36.0	36.0	36.0	32.0	36.0
44	34.758736842105264	36.0	36.0	36.0	32.0	36.0
45	34.73988818645746	36.0	36.0	36.0	32.0	36.0
46	34.72365115393404	36.0	36.0	36.0	32.0	36.0
47	34.698512910351134	36.0	36.0	36.0	32.0	36.0
48	34.66969121791454	36.0	36.0	36.0	32.0	36.0
49	34.64840703785513	36.0	36.0	36.0	32.0	36.0
50	34.2399439408942	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	4.0
20	20.0
21	40.0
22	149.0
23	345.0
24	817.0
25	1946.0
26	4018.0
27	7257.0
28	12402.0
29	19649.0
30	29534.0
31	42915.0
32	64954.0
33	110376.0
34	256576.0
35	1090120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32819281221047	18.230604183937192	12.534683278880381	25.90651972497196
2	16.12408457553189	19.93526064537044	37.14725156333598	26.793403215761693
3	18.374425239379498	23.208812529019852	28.854963506585147	29.56179872501551
4	12.716711229620675	16.076603596071962	35.28922860186068	35.91745657244669
5	14.713870058648792	36.583258435524414	33.11045776525249	15.592413740574301
6	34.87132583683602	34.963275125188744	16.375808745480228	13.789590292495014
7	30.632828090486708	30.392809810343515	20.06739279457689	18.906969304592884
8	28.247939675527455	33.225805468809504	18.96820778429431	19.558047071368726
9	27.06107091172214	14.437474293548632	18.692908827785818	39.80854596694341
10	16.60921623886054	26.867727930535455	30.80655038464468	25.716505445959324
11	37.02923299565847	21.40976464315637	21.936171833346027	19.624830527839134
12	24.27401934648488	23.77192474674385	28.56558763043644	23.38846827633483
13	29.466737756112423	19.476639500342753	25.19454642394699	25.862076319597836
14	23.527580166044633	19.630862975093304	25.05263157894737	31.788925279914693
15	25.151831822682613	27.300418919948203	22.089115698072966	25.458633559296214
16	25.704431756555014	26.037864616022084	23.529562308437583	24.728141318985323
17	24.055571635311143	26.292695559448546	24.960865260111202	24.6908675451291
18	24.637199870332772	25.3663952266861	25.933689349494614	24.062715553486512
19	25.561550765480995	25.10381597989184	25.32238555868688	24.012247695940285
20	25.745617936996762	24.554664430797953	25.057613023285292	24.642104608919997
21	26.543742859319064	24.3355015614289	24.76551146317313	24.355244116078907
22	25.845075786426996	24.296199253560818	25.134709421890474	24.724015538121716
23	24.516414045243355	24.395643232538657	25.634610404448168	25.45333231776982
24	24.69808489026005	24.912574256279452	25.431979755128705	24.957361098331795
25	24.921029540801403	24.47145851613532	25.46651067563615	25.14100126742712
26	24.614211871032058	25.36711187048365	25.701639306303438	24.317036952180857
27	25.08494240490594	24.93553151765388	25.145511092478756	24.834014984961417
28	24.777679334201856	24.794802016698576	25.65727336210269	24.770245286996875
29	24.627235823383813	25.048290250914317	25.505112977860218	24.819360947841655
30	24.65736563346227	25.04180102711095	25.64182247603448	24.659010863392293
31	25.140757531441942	24.771436579896655	24.97952617724481	25.108279711416593
32	25.00670277226661	24.869295940801113	24.908659494294113	25.215341792638164
33	24.621767681968606	24.625179983681885	25.39757885006651	25.355473484282996
34	25.184554628474697	24.821325527248884	25.563992241850503	24.430127602425912
35	25.5664864041435	24.59763881483738	25.563256912179146	24.272617868839973
36	24.491232217958203	25.133902293674694	25.2434614854959	25.131404002871204
37	25.376546677894922	24.899914569391957	25.13530575261197	24.58823300010115
38	24.820047223703252	24.667042425165665	25.49092847893975	25.021981872191333
39	25.412615058758725	24.68134527803607	24.902840624501103	25.003199038704103
40	25.548193365742133	24.912833812072012	25.149867346528403	24.38910547565745
41	24.644278878291004	25.257281230976968	25.555556232606975	24.542883658125053
42	25.579037234246755	25.190077286213707	25.07588161918975	24.155003860349787
43	25.130845068105245	24.431481302058483	25.3430246127053	25.094649017130973
44	24.711331455289205	25.01930144314468	25.167619399306183	25.10174770225993
45	25.022058429244847	25.14472035761467	25.012491651920428	24.820729561220062
46	24.84155415554352	25.010770234788072	25.06701310160216	25.080662508066247
47	24.95614240875214	24.936643375477647	25.39803925033529	24.709174965434915
48	25.58190834494164	25.35839509197071	24.448803399741386	24.610893163346265
49	24.91694514113842	25.433737700203036	24.762900619346727	24.88641653931182
50	25.065047913043585	25.552404548661933	24.65501755531967	24.727529982974815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	175.0
1	123.0
2	71.0
3	171.5
4	272.0
5	282.5
6	293.0
7	249.0
8	205.0
9	285.5
10	366.0
11	607.0
12	848.0
13	1607.5
14	2367.0
15	2960.5
16	3554.0
17	3576.5
18	3599.0
19	3443.0
20	3287.0
21	4045.0
22	4803.0
23	5841.5
24	6880.0
25	8675.0
26	10470.0
27	13136.0
28	15802.0
29	18481.0
30	21160.0
31	24160.5
32	27161.0
33	30945.5
34	34730.0
35	39959.5
36	45189.0
37	52336.0
38	59483.0
39	65164.5
40	70846.0
41	77290.5
42	83735.0
43	87324.0
44	90913.0
45	99006.5
46	107100.0
47	113530.0
48	119960.0
49	124443.0
50	128926.0
51	126600.5
52	124275.0
53	123209.5
54	122144.0
55	121751.5
56	121359.0
57	116563.5
58	111768.0
59	101867.0
60	91966.0
61	80287.5
62	68609.0
63	59325.0
64	50041.0
65	42085.5
66	34130.0
67	29362.0
68	24594.0
69	21755.5
70	18917.0
71	14969.0
72	11021.0
73	9577.0
74	8133.0
75	6100.0
76	4067.0
77	3576.5
78	3086.0
79	2639.5
80	2193.0
81	1675.0
82	1157.0
83	956.5
84	756.0
85	579.5
86	403.0
87	283.0
88	163.0
89	104.5
90	46.0
91	37.0
92	28.0
93	26.5
94	25.0
95	26.0
96	27.0
97	20.0
98	13.0
99	11.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03442760301622363
2	0.0050575062837992235
3	4.265366745372839E-4
4	6.0933810648183415E-5
5	0.0
6	1.8280143194455024E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.140071597227512E-4
17	0.0
18	6.0933810648183415E-5
19	0.0
20	1.8280143194455024E-4
21	0.0
22	0.0
23	0.0
24	0.0010968085916673014
25	3.046690532409171E-4
26	2.4373524259273366E-4
27	7.921395384263843E-4
28	0.0016452128875009521
29	4.265366745372839E-4
30	0.0010358747810191179
31	3.046690532409171E-4
32	7.921395384263843E-4
33	2.4373524259273366E-4
34	7.31205727778201E-4
35	0.0
36	1.8280143194455024E-4
37	0.0016452128875009521
38	0.0
39	4.265366745372839E-4
40	4.265366745372839E-4
41	0.001340543834260035
42	0.00499657247315104
43	0.0037778962601873714
44	0.0034122933962982714
45	0.0017670805087973188
46	0.0017061466981491357
47	0.0010968085916673014
48	0.0035950948282428216
49	0.00249828623657552
50	0.0016452128875009521
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1641125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.003436600726154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10729962708214	44.52384502346298
2	13.231373762662777	15.084675508409182
3	3.7900592120706205	6.481392000247972
4	1.6004541378825934	3.649255439246409
5	0.8149609854980657	2.3227788434452146
6	0.5217706255392712	1.7845631263829442
7	0.34749422427837284	1.3865855487939518
8	0.24515508862944474	1.1179746041627163
9	0.18991161753368502	0.974305336484049
>10	1.01876206042698	10.329685695972596
>50	0.07227602928634022	2.8436412120432806
>100	0.054025672822980486	6.363941421587646
>500	0.004950333153203921	2.037935037856668
>1k	0.0015066231335838021	1.099421201904434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2186762129636682E-4
2	0.0	0.0	0.0	0.0	1.2186762129636682E-4
3	0.0	0.0	0.0	0.0	1.2186762129636682E-4
4	0.0	0.0	0.0	0.0	1.2186762129636682E-4
5	0.0	0.0	0.0	0.0	1.2186762129636682E-4
6	0.0	0.0	0.0	0.0	4.2653667453728387E-4
7	0.0	0.0	0.0	0.0	4.2653667453728387E-4
8	0.0	0.0	0.0	0.0	4.2653667453728387E-4
9	0.0	0.0	0.0	0.0	4.2653667453728387E-4
10	0.0	0.0	0.0	0.0	4.2653667453728387E-4
11	0.0	0.0	0.0	6.093381064818341E-5	4.2653667453728387E-4
12	0.0	0.0	0.0	6.093381064818341E-5	6.093381064818342E-4
13	0.0	0.0	0.0	6.093381064818341E-5	6.702719171300175E-4
14	0.0	0.0	0.0	6.093381064818341E-5	6.702719171300175E-4
15	0.0	0.0	0.0	6.093381064818341E-5	7.921395384263843E-4
16	0.0	0.0	0.0	1.2186762129636682E-4	7.921395384263843E-4
17	0.0	0.0	0.0	1.2186762129636682E-4	7.921395384263843E-4
18	0.0	0.0	0.0	1.8280143194455024E-4	7.921395384263843E-4
19	0.0	0.0	0.0	6.093381064818342E-4	7.921395384263843E-4
20	0.0	0.0	0.0	8.530733490745677E-4	8.530733490745677E-4
21	0.0	0.0	0.0	9.749409703709345E-4	8.530733490745677E-4
22	0.0	0.0	0.0	0.0012796100236118517	8.530733490745677E-4
23	0.0	0.0	0.0	0.0018889481300936857	8.530733490745677E-4
24	0.0	0.0	0.0	0.0023154848046309695	8.530733490745677E-4
25	0.0	0.0	0.0	0.003107624343057354	8.530733490745677E-4
26	0.0	0.0	0.0	0.003716962449539188	9.140071597227512E-4
27	0.0	0.0	0.0	0.004509101987965573	9.140071597227512E-4
28	0.0	0.0	0.0	0.005484042958336507	9.140071597227512E-4
29	0.0	0.0	0.0	0.007129255845837459	9.140071597227512E-4
30	0.0	0.0	0.0	0.009749409703709347	9.140071597227512E-4
31	0.0	0.0	0.0	0.01498971741945312	9.749409703709345E-4
32	0.0	0.0	0.0	0.019925356081955975	9.749409703709345E-4
33	0.0	0.0	0.0	0.02589686952547795	9.749409703709345E-4
34	0.0	0.0	0.0	0.03314799299261178	0.001035874781019118
35	0.0	0.0	0.0	0.04143499124076472	0.001035874781019118
36	0.0	0.0	0.0	0.052768680021326834	0.001035874781019118
37	0.0	0.0	0.0	0.06745372838753903	0.001035874781019118
38	0.0	0.0	0.0	0.0851854672861604	0.001035874781019118
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	970	0.0	27.229372	1
GTATCAA	2105	0.0	26.140629	1
TAGGACG	155	1.8189894E-12	21.289366	4
TAACGGC	85	6.1147857E-6	20.706211	36
CGCAATA	335	0.0	19.045078	36
GTATAGA	215	0.0	18.42732	1
CGAGCCG	440	0.0	17.999739	15
TTAACGG	100	2.4835512E-5	17.60028	35
ATCAACG	3125	0.0	17.458414	3
TCTATAC	215	0.0	17.394567	3
TCAACGC	3140	0.0	17.375013	4
CAACGCA	3170	0.0	17.210583	5
ATACCGT	490	0.0	17.060457	6
TATCAAC	3275	0.0	16.860823	2
TACACCG	105	3.777127E-5	16.76115	5
GACGGCC	645	0.0	16.712934	11
AACGCAG	3265	0.0	16.709816	6
CAATACG	385	0.0	16.572199	38
GCATTCG	640	0.0	16.49976	22
CGGATCG	80	0.0019889516	16.49976	26
>>END_MODULE