FastQCFastQC Report
Thu 2 Feb 2017
SRR4062233_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062233_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT251.5346838551258442No Hit
GTACATGGGAAGCAGTGGTATCAAC120.7366482504604052No Hit
TATCAACGCAGAGTACTTTTTTTTT100.6138735420503376No Hit
CCCATGTACTCTGCGTTGATACCAC60.3683241252302026No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50.3069367710251688No Hit
GTCCTAAAGTGTGTATTTCTCATTT50.3069367710251688No Hit
GGTATCAACGCAGAGTACTTTTTTT50.3069367710251688No Hit
GCAGAGTACTTTTTTTTTTTTTTTT40.24554941682013504No Hit
ATCAACGCAGAGTACTTTTTTTTTT40.24554941682013504No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40.24554941682013504No Hit
CTTTAGGACGTGAAATATGGCGAGG40.24554941682013504No Hit
GAGCAGGGAGGAGTCCACGAGACTT30.1841620626151013No Hit
GTCCTACAGTGGACATTTCTAAATT30.1841620626151013No Hit
GAGTACATGGGAAGCAGTGGTATCA30.1841620626151013No Hit
CTTCCATGGTCAGCGGCTTCTCTTC30.1841620626151013No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30.1841620626151013No Hit
CTGTACATTTGCTAGCTTGTAGTTT20.12277470841006752No Hit
GTATCAACGCAGAGTACATGGGCTC20.12277470841006752No Hit
GTTTTGTGAAGTTCTAAAAGGTTCT20.12277470841006752No Hit
GGGAAGCAGTGGTATCAACGCAGAG20.12277470841006752No Hit
GAACTGTCTCTTACACATCATTATT20.12277470841006752No Hit
ATGTTCTACAGTGTGGTTTTTATCA20.12277470841006752No Hit
CCTCTGGCGTATGCGTTTCCAACGT20.12277470841006752No Hit
GGTATCAACGCAGAGTACATGGGAA20.12277470841006752No Hit
CGTGTGCACCGTGTGTGTGCAGTGC20.12277470841006752No Hit
GGCTGTGGATGACTCATATTTCCAA20.12277470841006752No Hit
GTGGTATCAACGCAGAGTACATGGG20.12277470841006752No Hit
GTGTATTTCTCATTTTCCGTGATTT20.12277470841006752No Hit
TCTATATGCTGATATATGCAGGAAA20.12277470841006752No Hit
GTACTCTGCGTTGATACCACTGCTT20.12277470841006752No Hit
GTATCAACGCAGAGTACATGGGAAG20.12277470841006752No Hit
GTATCAACGCAGAGTACATGGGACA20.12277470841006752No Hit
GTACATGGAAGCAGTGGTATCAACG20.12277470841006752No Hit
GTGCAGGGTTGACTCATTCTGGAAT20.12277470841006752No Hit
ACGCAGAGTACATGGGAAGCAGTGG20.12277470841006752No Hit
TATTAGATGTGTGAGTTACACTGAA20.12277470841006752No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)