##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062233_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1629
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.496623695518725	32.0	21.0	32.0	14.0	32.0
2	25.685082872928177	32.0	14.0	32.0	14.0	32.0
3	26.15960712093309	32.0	21.0	32.0	14.0	32.0
4	25.754450583179864	32.0	14.0	32.0	14.0	32.0
5	26.178023327194598	32.0	21.0	32.0	14.0	32.0
6	29.20319214241866	36.0	21.0	36.0	14.0	36.0
7	29.112952731737263	32.0	21.0	36.0	14.0	36.0
8	28.462860650705956	32.0	21.0	36.0	14.0	36.0
9	28.988950276243095	32.0	21.0	36.0	14.0	36.0
10	27.536525475751993	32.0	21.0	36.0	14.0	36.0
11	29.77900552486188	36.0	21.0	36.0	14.0	36.0
12	28.54941682013505	32.0	21.0	36.0	14.0	36.0
13	29.298342541436465	32.0	21.0	36.0	14.0	36.0
14	28.303253529772867	32.0	21.0	36.0	14.0	36.0
15	28.539594843462247	32.0	21.0	36.0	14.0	36.0
16	28.567833026396563	32.0	21.0	36.0	14.0	36.0
17	28.26642111724985	32.0	21.0	36.0	14.0	36.0
18	27.9073050951504	32.0	21.0	36.0	14.0	36.0
19	28.520564763658687	32.0	21.0	36.0	14.0	36.0
20	28.098833640270104	32.0	21.0	36.0	14.0	36.0
21	27.922038060159608	32.0	21.0	36.0	14.0	36.0
22	28.063842848373234	32.0	21.0	36.0	14.0	36.0
23	27.76795580110497	32.0	14.0	36.0	14.0	36.0
24	27.914671577655003	32.0	14.0	36.0	14.0	36.0
25	26.776550030693677	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	6.0
15	5.0
16	14.0
17	23.0
18	34.0
19	66.0
20	79.0
21	68.0
22	101.0
23	79.0
24	81.0
25	52.0
26	75.0
27	78.0
28	79.0
29	80.0
30	78.0
31	98.0
32	107.0
33	131.0
34	142.0
35	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71761817065684	22.406384284837326	11.233885819521179	26.64211172498465
2	20.073664825046038	19.2756292203806	39.71761817065684	20.933087783916513
3	21.301411909146715	27.19459791282996	26.764886433394718	24.739103744628608
4	11.970534069981584	15.776550030693679	38.30570902394107	33.94720687538367
5	12.70718232044199	40.331491712707184	32.47391037446286	14.487415592387967
6	31.57248157248157	38.14496314496314	16.89189189189189	13.39066339066339
7	27.781192378610942	31.591886908420406	22.372464658881377	18.254456054087278
8	27.105101413644743	34.17332513829133	19.54517516902274	19.17639827904118
9	27.88697788697789	15.47911547911548	19.84029484029484	36.79361179361179
10	14.505224339274738	27.473878303626304	33.31284572833436	24.708051628764597
11	34.23478795328826	23.540258143822985	22.372464658881377	19.852489244007376
12	23.970497848801475	26.121696373693915	29.993853718500308	19.913952059004302
13	29.668304668304668	19.901719901719904	26.65847665847666	23.771498771498774
14	21.573448063921326	21.081745543945914	29.502151198524896	27.842655193607868
15	24.892440073755377	28.02704363859865	24.708051628764597	22.372464658881377
16	21.020282728948985	26.67486170866626	29.56361401352182	22.741241548862938
17	22.433927473878303	25.814382298709283	27.842655193607868	23.90903503380455
18	22.126613398893667	24.46220036877689	30.977258758451136	22.433927473878303
19	22.48157248157248	23.771498771498774	30.22113022113022	23.525798525798525
20	23.832923832923832	25.675675675675674	29.668304668304668	20.823095823095823
21	23.832923832923832	24.877149877149876	28.07125307125307	23.21867321867322
22	21.976672805402085	26.151012891344383	29.22038060159607	22.65193370165746
23	22.283609576427256	26.45794966236955	27.37875997544506	23.879680785758133
24	21.375921375921376	25.614250614250615	29.975429975429975	23.034398034398034
25	21.06879606879607	25.7985257985258	29.545454545454547	23.587223587223587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	1.0
4	1.0
5	1.0
6	3.0
7	5.0
8	5.0
9	5.0
10	7.0
11	9.0
12	9.0
13	9.0
14	10.5
15	12.0
16	12.0
17	12.0
18	14.5
19	17.0
20	17.0
21	17.0
22	27.0
23	37.0
24	37.0
25	37.0
26	62.5
27	88.0
28	88.0
29	88.0
30	117.0
31	146.0
32	146.0
33	146.0
34	142.0
35	138.0
36	138.0
37	138.0
38	145.0
39	152.0
40	152.0
41	152.0
42	144.0
43	136.0
44	136.0
45	136.0
46	162.0
47	188.0
48	188.0
49	188.0
50	194.5
51	201.0
52	201.0
53	201.0
54	184.5
55	168.0
56	168.0
57	168.0
58	150.0
59	132.0
60	132.0
61	132.0
62	108.0
63	84.0
64	84.0
65	84.0
66	71.0
67	58.0
68	58.0
69	58.0
70	42.0
71	26.0
72	26.0
73	26.0
74	19.0
75	12.0
76	12.0
77	12.0
78	11.5
79	11.0
80	11.0
81	11.0
82	7.0
83	3.0
84	3.0
85	3.0
86	2.5
87	2.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.06138735420503376
7	0.12277470841006752
8	0.12277470841006752
9	0.06138735420503376
10	0.12277470841006752
11	0.12277470841006752
12	0.12277470841006752
13	0.06138735420503376
14	0.12277470841006752
15	0.12277470841006752
16	0.12277470841006752
17	0.12277470841006752
18	0.12277470841006752
19	0.06138735420503376
20	0.06138735420503376
21	0.06138735420503376
22	0.0
23	0.0
24	0.06138735420503376
25	0.06138735420503376
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1629.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.67710251688153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.64089121887287	91.4671577655003
2	1.310615989515072	2.4554941682013505
3	0.327653997378768	0.9208103130755065
4	0.2621231979030144	0.9821976672805401
5	0.19659239842726078	0.9208103130755065
6	0.0655307994757536	0.3683241252302026
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.19659239842726078	2.8852056476365866
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	25	1.5346838551258442	No Hit
GTACATGGGAAGCAGTGGTATCAAC	12	0.7366482504604052	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10	0.6138735420503376	No Hit
CCCATGTACTCTGCGTTGATACCAC	6	0.3683241252302026	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	5	0.3069367710251688	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5	0.3069367710251688	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5	0.3069367710251688	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	4	0.24554941682013504	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	4	0.24554941682013504	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4	0.24554941682013504	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4	0.24554941682013504	No Hit
GAGCAGGGAGGAGTCCACGAGACTT	3	0.1841620626151013	No Hit
GTCCTACAGTGGACATTTCTAAATT	3	0.1841620626151013	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3	0.1841620626151013	No Hit
CTTCCATGGTCAGCGGCTTCTCTTC	3	0.1841620626151013	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3	0.1841620626151013	No Hit
CTGTACATTTGCTAGCTTGTAGTTT	2	0.12277470841006752	No Hit
GTATCAACGCAGAGTACATGGGCTC	2	0.12277470841006752	No Hit
GTTTTGTGAAGTTCTAAAAGGTTCT	2	0.12277470841006752	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	2	0.12277470841006752	No Hit
GAACTGTCTCTTACACATCATTATT	2	0.12277470841006752	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	2	0.12277470841006752	No Hit
CCTCTGGCGTATGCGTTTCCAACGT	2	0.12277470841006752	No Hit
GGTATCAACGCAGAGTACATGGGAA	2	0.12277470841006752	No Hit
CGTGTGCACCGTGTGTGTGCAGTGC	2	0.12277470841006752	No Hit
GGCTGTGGATGACTCATATTTCCAA	2	0.12277470841006752	No Hit
GTGGTATCAACGCAGAGTACATGGG	2	0.12277470841006752	No Hit
GTGTATTTCTCATTTTCCGTGATTT	2	0.12277470841006752	No Hit
TCTATATGCTGATATATGCAGGAAA	2	0.12277470841006752	No Hit
GTACTCTGCGTTGATACCACTGCTT	2	0.12277470841006752	No Hit
GTATCAACGCAGAGTACATGGGAAG	2	0.12277470841006752	No Hit
GTATCAACGCAGAGTACATGGGACA	2	0.12277470841006752	No Hit
GTACATGGAAGCAGTGGTATCAACG	2	0.12277470841006752	No Hit
GTGCAGGGTTGACTCATTCTGGAAT	2	0.12277470841006752	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2	0.12277470841006752	No Hit
TATTAGATGTGTGAGTTACACTGAA	2	0.12277470841006752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE