##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062233_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1629
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.787599754450582	32.0	32.0	32.0	14.0	32.0
2	28.515039901780234	32.0	32.0	32.0	14.0	32.0
3	30.38121546961326	32.0	32.0	32.0	21.0	32.0
4	30.87415592387968	32.0	32.0	32.0	32.0	32.0
5	30.988336402701044	32.0	32.0	32.0	32.0	32.0
6	34.208717004297114	36.0	36.0	36.0	32.0	36.0
7	34.23020257826888	36.0	36.0	36.0	32.0	36.0
8	33.63351749539595	36.0	36.0	36.0	21.0	36.0
9	34.173112338858196	36.0	36.0	36.0	32.0	36.0
10	33.50399017802333	36.0	36.0	36.0	21.0	36.0
11	34.18968692449356	36.0	36.0	36.0	32.0	36.0
12	34.19582565991406	36.0	36.0	36.0	32.0	36.0
13	34.209330877839164	36.0	36.0	36.0	32.0	36.0
14	33.94659300184162	36.0	36.0	36.0	32.0	36.0
15	33.93922651933702	36.0	36.0	36.0	32.0	36.0
16	34.135052179251076	36.0	36.0	36.0	32.0	36.0
17	33.96071209330878	36.0	36.0	36.0	32.0	36.0
18	34.25230202578269	36.0	36.0	36.0	32.0	36.0
19	34.07182320441989	36.0	36.0	36.0	32.0	36.0
20	33.93124616329036	36.0	36.0	36.0	32.0	36.0
21	34.04051565377532	36.0	36.0	36.0	32.0	36.0
22	33.96869244935543	36.0	36.0	36.0	32.0	36.0
23	33.74769797421731	36.0	36.0	36.0	32.0	36.0
24	34.04481276856968	36.0	36.0	36.0	32.0	36.0
25	33.866789441375076	36.0	36.0	36.0	32.0	36.0
26	33.46470227133211	36.0	36.0	36.0	21.0	36.0
27	33.7243707796194	36.0	36.0	36.0	32.0	36.0
28	33.61878453038674	36.0	36.0	36.0	21.0	36.0
29	33.48127685696746	36.0	36.0	36.0	21.0	36.0
30	33.54573357888275	36.0	36.0	36.0	27.0	36.0
31	33.43830570902394	36.0	36.0	36.0	21.0	36.0
32	33.445672191528544	36.0	36.0	36.0	21.0	36.0
33	33.483732351135664	36.0	36.0	36.0	21.0	36.0
34	33.208717004297114	36.0	36.0	36.0	21.0	36.0
35	33.365868631062	36.0	36.0	36.0	21.0	36.0
36	33.02394106813996	36.0	36.0	36.0	14.0	36.0
37	33.087170042971145	36.0	36.0	36.0	21.0	36.0
38	33.08839779005525	36.0	36.0	36.0	21.0	36.0
39	32.96439533456108	36.0	36.0	36.0	14.0	36.0
40	32.88397790055249	36.0	36.0	36.0	14.0	36.0
41	32.78023327194598	36.0	36.0	36.0	14.0	36.0
42	33.042357274401475	36.0	36.0	36.0	14.0	36.0
43	33.06752608962554	36.0	36.0	36.0	14.0	36.0
44	32.500306936771025	36.0	36.0	36.0	14.0	36.0
45	32.415592387968076	36.0	36.0	36.0	14.0	36.0
46	32.699815837937386	36.0	36.0	36.0	14.0	36.0
47	32.10926949048496	36.0	32.0	36.0	14.0	36.0
48	32.10067526089625	36.0	32.0	36.0	14.0	36.0
49	32.268262737876	36.0	32.0	36.0	14.0	36.0
50	31.52302025782689	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	5.0
24	6.0
25	9.0
26	15.0
27	28.0
28	47.0
29	63.0
30	94.0
31	145.0
32	206.0
33	280.0
34	381.0
35	349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75184275184275	20.945945945945947	12.285012285012286	24.01719901719902
2	17.311233885819522	23.081645181092693	35.91160220994475	23.69551872314303
3	22.283609576427256	26.45794966236955	27.255985267034994	24.002455494168203
4	14.303253529772867	16.63597298956415	37.01657458563536	32.04419889502763
5	17.495395948434624	34.683855125844076	33.087783916513196	14.732965009208105
6	31.00061387354205	39.77900552486188	16.758747697974215	12.461632903621854
7	27.992633517495396	32.28974831184776	19.643953345610804	20.073664825046038
8	26.27378759975445	33.210558624923266	19.828115408225905	20.687538367096376
9	24.554941682013506	14.671577655003068	18.41620626151013	42.35727440147329
10	15.592387968078574	26.27378759975445	32.65807243707796	25.475751995089013
11	34.19275629220381	21.915285451197054	24.677716390423573	19.21424186617557
12	24.554941682013506	23.388581952117864	30.14119091467158	21.915285451197054
13	29.036218538980968	20.50337630448128	25.10742786985881	25.352977286678946
14	21.608348680171886	20.56476365868631	26.45794966236955	31.36893799877225
15	24.1866175567833	28.422344996930633	22.22222222222222	25.168815224063845
16	21.485573971761816	27.255985267034994	26.028238182934317	25.23020257826888
17	23.02025782688766	24.677716390423573	27.86985880908533	24.432166973603437
18	23.081645181092693	25.905463474524247	28.115408225905465	22.897483118477595
19	22.467771639042358	25.659914057704114	28.299570288520563	23.572744014732965
20	22.65193370165746	27.931246163290364	24.92326580724371	24.49355432780847
21	21.424186617556785	27.992633517495396	26.212400245549418	24.3707796193984
22	21.85389809699202	27.13321055862492	25.41436464088398	25.59852670349908
23	22.529158993247393	29.711479435236342	24.61632903621854	23.143032535297728
24	24.49355432780847	27.992633517495396	22.65193370165746	24.861878453038674
25	23.081645181092693	28.974831184775933	24.92326580724371	23.02025782688766
26	22.83609576427256	27.992633517495396	27.13321055862492	22.03806015960712
27	21.669736034376918	29.588704726826276	26.887661141804784	21.85389809699202
28	20.994475138121548	27.010435850214854	28.115408225905465	23.879680785758133
29	24.1866175567833	26.27378759975445	25.10742786985881	24.432166973603437
30	21.240024554941684	27.19459791282996	27.624309392265197	23.941068139963168
31	23.02025782688766	27.685696746470228	26.151012891344383	23.143032535297728
32	21.792510742786988	29.588704726826276	26.519337016574585	22.099447513812155
33	22.283609576427256	28.974831184775933	26.028238182934317	22.713321055862494
34	23.69551872314303	27.440147329650095	25.905463474524247	22.958870472682626
35	21.240024554941684	30.939226519337016	25.168815224063845	22.65193370165746
36	21.54696132596685	27.501534683855127	27.501534683855127	23.4499693063229
37	22.529158993247393	26.335174953959484	26.089625537139348	25.04604051565378
38	20.933087783916513	29.588704726826276	26.27378759975445	23.204419889502763
39	22.65193370165746	27.624309392265197	25.41436464088398	24.30939226519337
40	23.69551872314303	27.931246163290364	25.59852670349908	22.774708410067525
41	21.608348680171886	29.2817679558011	25.78268876611418	23.32719459791283
42	21.976672805402085	27.317372621240025	26.764886433394718	23.941068139963168
43	22.406384284837326	29.158993247391034	26.335174953959484	22.099447513812155
44	21.85389809699202	29.158993247391034	27.56292203806016	21.424186617556785
45	23.265807243707794	30.14119091467158	24.248004910988335	22.34499693063229
46	22.283609576427256	28.23818293431553	24.063842848373234	25.41436464088398
47	22.65193370165746	28.974831184775933	24.92326580724371	23.4499693063229
48	22.406384284837326	27.37875997544506	26.39656230816452	23.8182934315531
49	22.406384284837326	27.624309392265197	26.703499079189687	23.265807243707794
50	22.099447513812155	28.974831184775933	25.04604051565378	23.879680785758133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	3.0
4	3.0
5	5.0
6	7.0
7	5.0
8	3.0
9	2.0
10	1.0
11	1.5
12	2.0
13	5.0
14	8.0
15	10.0
16	12.0
17	10.0
18	8.0
19	11.5
20	15.0
21	18.5
22	22.0
23	19.5
24	17.0
25	24.5
26	32.0
27	30.5
28	29.0
29	36.0
30	43.0
31	51.5
32	60.0
33	63.5
34	67.0
35	70.5
36	74.0
37	76.0
38	78.0
39	76.5
40	75.0
41	76.5
42	78.0
43	79.5
44	81.0
45	78.5
46	76.0
47	78.0
48	80.0
49	85.5
50	91.0
51	89.0
52	87.0
53	84.0
54	81.0
55	81.5
56	82.0
57	81.5
58	81.0
59	79.0
60	77.0
61	66.5
62	56.0
63	56.0
64	56.0
65	47.0
66	38.0
67	33.5
68	29.0
69	22.5
70	16.0
71	16.0
72	16.0
73	16.5
74	17.0
75	11.5
76	6.0
77	5.0
78	4.0
79	6.5
80	9.0
81	4.5
82	0.0
83	2.0
84	4.0
85	2.0
86	0.0
87	1.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06138735420503376
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1629.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.64947820748927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.69321717486	97.36034376918354
2	1.2445550715619167	2.4554941682013505
3	0.06222775357809583	0.1841620626151013
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.1841620626151013	No Hit
CTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC	2	0.12277470841006752	No Hit
CCCCGAGTCTGCCTCGGTGCTTTCTTCGCTGCTCTTTATTTCTTGACATG	2	0.12277470841006752	No Hit
GTATAAGAAAAGTGCCTTTCTTTCCAGGTCTCTGTGCACATCTGACTCCC	2	0.12277470841006752	No Hit
ACTCAGACCCGCGCGGGCCCACACTCGATGCGGTATTTCGAGACCGCCGT	2	0.12277470841006752	No Hit
GAGAAACCCTGTCTCAAAAAACAAACAAACAAAACCAAAAGCCTAAACAA	2	0.12277470841006752	No Hit
CCATGGCATATCCCCAAATTTAGCTAAAATAATCAGTCTCAAATAATGAT	2	0.12277470841006752	No Hit
GCTGCGGGAGATCCGCCTGCAGCCGCAGGAGGCGCCGCTGGCCGCCGAGC	2	0.12277470841006752	No Hit
AAAGTCTATAACTGTATAATTACAGAAAAATAAGGAGATGGACAAGCGCA	2	0.12277470841006752	No Hit
TACATGAACTGTACAAATTTACACCAGTTCATAATTTACCAAATAAAAGA	2	0.12277470841006752	No Hit
GTATAATACCTTGGAATACTGCCTGTTGCATGTGCTGGGCACATGCCTCC	2	0.12277470841006752	No Hit
GCCCCACAGTGCGAGAGCGCGAGCGCTCACCCGGCTCACCGCTGCCTCCT	2	0.12277470841006752	No Hit
CTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCC	2	0.12277470841006752	No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGAATGTGTTTTTCAGTG	2	0.12277470841006752	No Hit
GTACATGGGAGACGCAGGCTAGGGAGCCTGTGGCTGCGGACATGAACTCG	2	0.12277470841006752	No Hit
AGGTGGGCCTCCTCGACCTCCAATGGGAATGAGACCTCCTGTAATGTCGC	2	0.12277470841006752	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.12277470841006752	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2	0.12277470841006752	No Hit
GAAGCAAGCTTTCTTACTACAGCAGCTTCCTGGGGCACACAGAGTATCTC	2	0.12277470841006752	No Hit
CTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCACGCTCCCGGGAA	2	0.12277470841006752	No Hit
GTTCCAGTAGTGACTGATTCACACTCTTTTCCAAGTGCAGTGCACACTCC	2	0.12277470841006752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE