FastQCFastQC Report
Thu 2 Feb 2017
SRR4062232_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062232_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109682
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21260.1915864184514122No Hit
GCGCAAGACGGACCAGAGCGAAAGC15720.1416622059292662No Hit
TATCAACGCAGAGTACTTTTTTTTT12790.11525824515491824No Hit
GGGTAGGCACACGCTGAGCCAGTCA12540.11300534747792611No Hit
GAATAGGACCGCGGTTCTATTTTGT11720.10561584309739186No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC11560.10417398858411689No Hit
TCGTAGTTCCGACCATAAACGATGC11270.10156062727880599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5050.014.2904791
GTATTAA551.9669865E-413.811851
GTGTTAG707.2924886E-613.565211
CGCAAGA2600.013.1531582
CAAGACG2750.013.1277724
AAGACGG2900.012.7763485
GTATCAA14600.012.747581
ACTCTAA1950.012.66829210
CGGCGTC751.4764133E-512.6677214
AACCGCG751.4764133E-512.667727
TCGGCGT751.4764133E-512.6677213
ATCGCCA1700.012.29458516
GCAAGAC2950.012.2372023
TCTATAC550.00306765412.0908223
GCGCAAG3000.012.0278191
GTATATG951.0446347E-611.9945011
GCGGTCG802.8608627E-511.8765239
TCGCCAG1850.011.8096617
CGGTTCT2900.011.79461712
GGTTCTA2900.011.79408513
Produced by FastQC (version 0.11.5)