##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062232_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109682
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33628372813112	32.0	32.0	32.0	32.0	32.0
2	30.952544062172766	32.0	32.0	32.0	32.0	32.0
3	30.974131327713707	32.0	32.0	32.0	32.0	32.0
4	30.973468074637598	32.0	32.0	32.0	32.0	32.0
5	30.92065654845262	32.0	32.0	32.0	32.0	32.0
6	34.5851505205996	36.0	36.0	36.0	32.0	36.0
7	34.52912275769094	36.0	36.0	36.0	32.0	36.0
8	34.481955190766364	36.0	36.0	36.0	32.0	36.0
9	34.59476408556686	36.0	36.0	36.0	32.0	36.0
10	34.34377506348665	36.0	36.0	36.0	32.0	36.0
11	34.57634619647791	36.0	36.0	36.0	32.0	36.0
12	34.43353501273338	36.0	36.0	36.0	32.0	36.0
13	34.504016465978545	36.0	36.0	36.0	32.0	36.0
14	34.4132481197316	36.0	36.0	36.0	32.0	36.0
15	34.383228708765216	36.0	36.0	36.0	32.0	36.0
16	34.377725330319855	36.0	36.0	36.0	32.0	36.0
17	34.323973895224036	36.0	36.0	36.0	32.0	36.0
18	34.32692879581718	36.0	36.0	36.0	32.0	36.0
19	34.32986387091076	36.0	36.0	36.0	32.0	36.0
20	34.31120807582713	36.0	36.0	36.0	32.0	36.0
21	34.29916228252779	36.0	36.0	36.0	32.0	36.0
22	34.269451067963615	36.0	36.0	36.0	32.0	36.0
23	34.243847336444134	36.0	36.0	36.0	32.0	36.0
24	34.216325037262926	36.0	36.0	36.0	32.0	36.0
25	33.89159777305571	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	16.0
5	90.0
6	233.0
7	49.0
8	188.0
9	153.0
10	85.0
11	23.0
12	46.0
13	43.0
14	146.0
15	244.0
16	348.0
17	527.0
18	684.0
19	875.0
20	1241.0
21	1806.0
22	2613.0
23	3933.0
24	5402.0
25	7793.0
26	10786.0
27	14121.0
28	18819.0
29	25455.0
30	33520.0
31	46196.0
32	66744.0
33	97439.0
34	224291.0
35	545772.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.43762796666378	17.25792672376503	11.74833102632869	25.556114283242493
2	17.229232263773817	19.06246676963948	36.91088766290762	26.797413303679086
3	19.89324783066723	22.069474520872365	27.746822712636966	30.290454935823437
4	13.395465958639535	14.93382073286576	34.43385768438357	37.236855624111136
5	15.673820113909468	35.56263307917952	32.65278526496842	16.11076154194259
6	35.601158529547554	34.11088628231764	16.103001644736842	14.184953543397969
7	31.58475417230492	29.623184483536306	19.262877762742445	19.52918358141633
8	28.533203424540133	32.65311645782024	18.58967766380687	20.22400245383276
9	26.72795842506045	14.891461258074996	18.669114728066695	39.711465588797864
10	17.04922500241383	26.212667964269894	30.04204126161684	26.696065771699434
11	37.52895599591029	21.343467969502242	21.25097121591269	19.876604818674778
12	25.063030480222775	23.44282679747409	27.7057988041805	23.78834391812264
13	29.40465781877799	19.301318160368208	24.88091983394193	26.413104186911873
14	23.707708144810667	19.583922396176828	24.40502021383996	32.30334924517254
15	25.47512871923236	27.045115346508748	21.646848206051832	25.83290772820706
16	26.143534332645835	25.863932541895156	23.034351592710685	24.958181532748323
17	24.28445626616188	26.129684801654403	24.538986103266392	25.046872828917333
18	25.033403071548882	24.966551819644163	25.56108589528802	24.438959213518928
19	25.605580341437808	24.993730865609603	25.019709436896427	24.380979356056166
20	26.06833636551414	24.1209456796159	24.652396625484933	25.158321329385036
21	26.74589666366307	24.01956937630731	24.436013930192093	24.798520029837526
22	26.212566653165386	24.092023991982224	24.80918543990864	24.88622391494375
23	24.658407724539988	24.252104569174353	25.321029096897668	25.768458609387995
24	24.788073293536755	24.86104571555368	25.243136172492736	25.107744818416833
25	24.972016599725983	24.28039141583829	25.05544817185641	25.692143812579317
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	110.0
1	110.0
2	225.0
3	340.0
4	340.0
5	340.0
6	708.5
7	1077.0
8	1077.0
9	1077.0
10	1333.5
11	1590.0
12	1590.0
13	1590.0
14	2170.5
15	2751.0
16	2751.0
17	2751.0
18	4481.0
19	6211.0
20	6211.0
21	6211.0
22	9920.0
23	13629.0
24	13629.0
25	13629.0
26	19930.0
27	26231.0
28	26231.0
29	26231.0
30	33662.5
31	41094.0
32	41094.0
33	41094.0
34	51745.0
35	62396.0
36	62396.0
37	62396.0
38	74108.0
39	85820.0
40	85820.0
41	85820.0
42	99767.0
43	113714.0
44	113714.0
45	113714.0
46	127465.0
47	141216.0
48	141216.0
49	141216.0
50	147716.5
51	154217.0
52	154217.0
53	154217.0
54	145068.5
55	135920.0
56	135920.0
57	135920.0
58	125866.0
59	115812.0
60	115812.0
61	115812.0
62	102920.0
63	90028.0
64	90028.0
65	90028.0
66	73780.5
67	57533.0
68	57533.0
69	57533.0
70	43339.5
71	29146.0
72	29146.0
73	29146.0
74	22318.0
75	15490.0
76	15490.0
77	15490.0
78	12332.5
79	9175.0
80	9175.0
81	9175.0
82	6346.0
83	3517.0
84	3517.0
85	3517.0
86	2560.0
87	1603.0
88	1603.0
89	1603.0
90	1066.5
91	530.0
92	530.0
93	530.0
94	316.0
95	102.0
96	102.0
97	102.0
98	266.0
99	430.0
100	430.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001622086327434346
2	3.604636283187436E-4
3	8.11043163717173E-4
4	0.010813908849562308
5	0.03424404469028064
6	0.06217997588498326
7	0.10651700216818873
8	0.1092204793805793
9	0.11913322915934475
10	0.13382212201333354
11	0.13832791736731787
12	0.13409246973457262
13	0.1257116903761618
14	0.115618708783237
15	0.13120876070802265
16	0.11895299734518539
17	0.12291809725669155
18	0.11273499975668703
19	0.09696471601774202
20	0.09182810931419993
21	0.09110718205756244
22	0.10318271360624034
23	0.08146478000003606
24	0.09408100699119207
25	0.08912463210180935
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1109682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.33590502597782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.36684384676794	41.494615743916626
2	13.915742102238982	15.122488824665167
3	4.302990635577995	7.014206715073139
4	1.8665725966643758	4.056876453457934
5	0.9484937014216768	2.5768631839093206
6	0.5918182135079978	1.929418694508866
7	0.37543452019709966	1.4279702103032217
8	0.2751331899622726	1.1959688703427487
9	0.1978522912952134	0.9675434978089934
>10	1.0028232963633739	9.754511520571317
>50	0.07674853469254822	2.935645478219791
>100	0.07072700041672088	7.698689647775294
>500	0.006656657278344472	2.387642403971705
>1k	0.0021634136154619534	1.4375587554759388
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2126	0.1915864184514122	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1572	0.1416622059292662	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1279	0.11525824515491824	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1254	0.11300534747792611	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1172	0.10561584309739186	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1156	0.10417398858411689	No Hit
TCGTAGTTCCGACCATAAACGATGC	1127	0.10156062727880599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8023181415937177E-4
2	0.0	0.0	0.0	0.0	1.8023181415937177E-4
3	0.0	0.0	0.0	0.0	1.8023181415937177E-4
4	0.0	0.0	0.0	0.0	1.8023181415937177E-4
5	0.0	0.0	0.0	0.0	1.8023181415937177E-4
6	0.0	0.0	0.0	0.0	3.6046362831874355E-4
7	0.0	0.0	0.0	9.011590707968589E-5	3.6046362831874355E-4
8	0.0	0.0	0.0	9.011590707968589E-5	3.6046362831874355E-4
9	0.0	0.0	0.0	9.011590707968589E-5	3.6046362831874355E-4
10	0.0	0.0	0.0	9.011590707968589E-5	3.6046362831874355E-4
11	0.0	0.0	0.0	9.011590707968589E-5	3.6046362831874355E-4
12	0.0	0.0	0.0	9.011590707968589E-5	4.5057953539842947E-4
13	0.0	0.0	0.0	9.011590707968589E-5	9.011590707968589E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	505	0.0	14.290479	1
GTATTAA	55	1.9669865E-4	13.81185	1
GTGTTAG	70	7.2924886E-6	13.56521	1
CGCAAGA	260	0.0	13.153158	2
CAAGACG	275	0.0	13.127772	4
AAGACGG	290	0.0	12.776348	5
GTATCAA	1460	0.0	12.74758	1
ACTCTAA	195	0.0	12.668292	10
CGGCGTC	75	1.4764133E-5	12.66772	14
AACCGCG	75	1.4764133E-5	12.66772	7
TCGGCGT	75	1.4764133E-5	12.66772	13
ATCGCCA	170	0.0	12.294585	16
GCAAGAC	295	0.0	12.237202	3
TCTATAC	55	0.003067654	12.090822	3
GCGCAAG	300	0.0	12.027819	1
GTATATG	95	1.0446347E-6	11.994501	1
GCGGTCG	80	2.8608627E-5	11.876523	9
TCGCCAG	185	0.0	11.80966	17
CGGTTCT	290	0.0	11.794617	12
GGTTCTA	290	0.0	11.794085	13
>>END_MODULE