FastQCFastQC Report
Thu 2 Feb 2017
SRR4062232_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062232_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109682
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15400.13877849690271626No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT11840.1066972339823481No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11720.10561584309739186No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11330.10210132272128411No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11160.10056935230092945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC407.033324E-427.4989594
GTATCAA15150.025.7112661
GGTATCA6800.025.2434391
TACCGTA550.00448436619.9992437
CGAATTG600.007413347718.3326414
CCTATAT750.001290717917.6001262
GGCGTTA3400.017.47228842
CGCAATA2800.017.28583736
TAGCGGC2950.017.15266630
ATCAACG22800.017.07833
ATACGAA2850.016.98410840
CTAGCGG2850.016.98257829
AACGCAG23100.016.8565046
TCAACGC23250.016.7477514
CGCCCCT2500.016.7193665
CAACGCA23500.016.6631985
GAGTATA800.001983928716.5053231
TACGAAT2950.016.40837741
GCGCAAT2950.016.40615835
GTATTAG1753.608875E-916.3481311
Produced by FastQC (version 0.11.5)