##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062232_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109682
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310628630544606	32.0	32.0	32.0	32.0	32.0
2	31.502886412503763	32.0	32.0	32.0	32.0	32.0
3	31.604208232628807	32.0	32.0	32.0	32.0	32.0
4	31.674515762173307	32.0	32.0	32.0	32.0	32.0
5	31.657641558572635	32.0	32.0	32.0	32.0	32.0
6	35.309290409324475	36.0	36.0	36.0	36.0	36.0
7	35.31455858525235	36.0	36.0	36.0	36.0	36.0
8	35.267841597863175	36.0	36.0	36.0	36.0	36.0
9	35.34368945337493	36.0	36.0	36.0	36.0	36.0
10	35.23773297214878	36.0	36.0	36.0	36.0	36.0
11	35.345104273116085	36.0	36.0	36.0	36.0	36.0
12	35.27260872934769	36.0	36.0	36.0	36.0	36.0
13	35.29962007133575	36.0	36.0	36.0	36.0	36.0
14	35.26918973183308	36.0	36.0	36.0	36.0	36.0
15	35.25155765345387	36.0	36.0	36.0	36.0	36.0
16	35.2609315101083	36.0	36.0	36.0	36.0	36.0
17	35.23642629149612	36.0	36.0	36.0	36.0	36.0
18	35.236260478227095	36.0	36.0	36.0	36.0	36.0
19	35.22704702788727	36.0	36.0	36.0	36.0	36.0
20	35.220569496486384	36.0	36.0	36.0	36.0	36.0
21	35.214768735547665	36.0	36.0	36.0	36.0	36.0
22	35.202844598722876	36.0	36.0	36.0	36.0	36.0
23	35.17684706068946	36.0	36.0	36.0	36.0	36.0
24	35.16034503578503	36.0	36.0	36.0	36.0	36.0
25	35.14602832162728	36.0	36.0	36.0	36.0	36.0
26	35.10186792252195	36.0	36.0	36.0	36.0	36.0
27	35.0862057778715	36.0	36.0	36.0	36.0	36.0
28	35.07082209137393	36.0	36.0	36.0	36.0	36.0
29	35.05708572365777	36.0	36.0	36.0	36.0	36.0
30	35.03410977198873	36.0	36.0	36.0	36.0	36.0
31	35.04121270778475	36.0	36.0	36.0	36.0	36.0
32	35.00926121177058	36.0	36.0	36.0	36.0	36.0
33	34.997520911396236	36.0	36.0	36.0	36.0	36.0
34	34.982556263866584	36.0	36.0	36.0	36.0	36.0
35	34.96845312440861	36.0	36.0	36.0	36.0	36.0
36	34.95223947040684	36.0	36.0	36.0	36.0	36.0
37	34.93283571329444	36.0	36.0	36.0	36.0	36.0
38	34.91385369862717	36.0	36.0	36.0	36.0	36.0
39	34.90033360908801	36.0	36.0	36.0	36.0	36.0
40	34.892277246995086	36.0	36.0	36.0	36.0	36.0
41	34.86216862128069	36.0	36.0	36.0	32.0	36.0
42	34.83012881167758	36.0	36.0	36.0	32.0	36.0
43	34.82819131967537	36.0	36.0	36.0	32.0	36.0
44	34.77774894068751	36.0	36.0	36.0	32.0	36.0
45	34.762572520776224	36.0	36.0	36.0	32.0	36.0
46	34.745608201268475	36.0	36.0	36.0	32.0	36.0
47	34.72189329916138	36.0	36.0	36.0	32.0	36.0
48	34.69952202522885	36.0	36.0	36.0	32.0	36.0
49	34.679555043697206	36.0	36.0	36.0	32.0	36.0
50	34.279423294241056	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	15.0
21	19.0
22	72.0
23	213.0
24	501.0
25	1176.0
26	2466.0
27	4609.0
28	8017.0
29	12874.0
30	19430.0
31	28836.0
32	43460.0
33	73865.0
34	171675.0
35	742452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1020782363027	17.737396173823996	12.147144250549442	26.013381339323864
2	16.660944012616874	19.439179903120422	36.66463895460178	27.235237129660923
3	19.317821353903827	22.423401340927114	27.911019392978154	30.347757912190904
4	13.048603113324358	15.370709806953704	34.52070052501527	37.05998655470666
5	15.194200129767141	35.826814937639675	32.675365871242164	16.30361906135102
6	35.86964553809109	34.07733026218322	15.925012751400851	14.128011448324834
7	31.467303245434273	29.657865947181268	19.369242719986445	19.505588087398014
8	28.65721891496843	32.68485926598792	18.485836482884284	20.172085336159366
9	26.799659722334866	14.68096265416579	18.66165261759675	39.857725005902594
10	17.042014777219762	26.20428753849079	30.067109376478466	26.686588307810982
11	37.69034732472907	21.26978720029702	21.2299559693678	19.80990950560611
12	25.082050533396057	23.443382879059047	27.618452854060894	23.85611373348401
13	29.436361047579396	19.2044207259377	24.932908707179173	26.426309519303732
14	23.84358762239993	19.43764069345993	24.464846685807284	32.253924998332856
15	25.526502187113064	26.945737607711038	21.70387552469987	25.823884680476027
16	26.344384957343216	25.735736087543064	22.982127134979645	24.93775182013408
17	24.293446230541722	26.127845635055806	24.602543791825045	24.976164342577423
18	25.206839094909427	24.90368836393227	25.535672928837975	24.35379961232032
19	25.76305644319724	24.843874190984444	25.09791093304208	24.295158432776237
20	26.18822757592743	23.961635717748752	24.758984137740857	25.09115256858296
21	26.980792695565036	23.906398409634473	24.42546603441346	24.68734286038703
22	26.367193484259456	23.98957539186902	24.921373871072973	24.72185725279855
23	24.706807896316242	24.08753138286464	25.404124785298855	25.801535935520263
24	24.88498822621881	24.742603424985287	25.28609882766843	25.08630952112747
25	25.209947723715192	24.066285716345508	25.195258817624165	25.52850774231513
26	24.771420177745256	25.27111468371906	25.37889369709051	24.578571441445177
27	25.391059388399157	24.416181991532678	25.090071173799966	25.102687446268195
28	25.086309832246666	24.366272703924157	25.3573826334975	25.190034830331676
29	24.704283584967893	24.667786511041943	25.4971261933642	25.130803710625965
30	24.8260069876567	24.79816089633774	25.52513312504337	24.850698990962186
31	25.328721781203	24.583730220595722	24.77531750230022	25.312230495901062
32	25.170026953789254	24.545701136456824	24.69024770270989	25.59402420704403
33	24.756055799870232	24.307908586259103	25.193659433350156	25.74237618052051
34	25.540474049135153	24.54099131817092	25.16360660878781	24.75492802390612
35	25.726762916549887	24.188122532511596	25.519315911509704	24.56579863942881
36	24.615452548975785	24.897335360342296	24.97303279050466	25.514179300177258
37	25.712488521331174	24.54195718174912	24.89882099765065	24.84673329926906
38	24.98238234016592	24.3055217620904	25.2406545298563	25.471441367887376
39	25.803681258026	24.273052920900266	24.54169013449884	25.381575686574898
40	25.662036511360913	24.62444195724541	24.95471675669246	24.758804774701222
41	24.736835939365704	25.02061425524188	25.54121994937229	24.70132985602013
42	25.822844814378897	24.902174246097335	25.024738064897544	24.250242874626228
43	25.535331761050216	24.04430630141875	25.086356914275726	25.334005023255312
44	24.91341809933465	24.880704875351803	24.764180912239343	25.4416961130742
45	25.048731274619097	24.95410744040726	24.916798614346593	25.08036267062705
46	24.958365475426618	24.812194049327	24.85103491532511	25.378405559921273
47	25.072206086308462	24.593325409062686	25.309934205724037	25.02453429890481
48	25.792288268023512	25.112424457073235	24.2838629674289	24.811424307474354
49	25.002861296081512	25.1544423987304	24.456556513526493	25.3861397916616
50	25.246675768594745	25.29344706237524	24.509599830577972	24.950277338452043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	34.0
2	37.0
3	97.5
4	158.0
5	166.0
6	174.0
7	134.0
8	94.0
9	154.5
10	215.0
11	400.0
12	585.0
13	994.0
14	1403.0
15	1832.0
16	2261.0
17	2323.0
18	2385.0
19	2287.0
20	2189.0
21	2501.5
22	2814.0
23	3322.5
24	3831.0
25	4784.5
26	5738.0
27	7187.5
28	8637.0
29	10249.5
30	11862.0
31	13809.0
32	15756.0
33	18238.0
34	20720.0
35	23896.5
36	27073.0
37	31934.5
38	36796.0
39	40846.0
40	44896.0
41	49093.5
42	53291.0
43	56544.5
44	59798.0
45	65604.5
46	71411.0
47	76638.5
48	81866.0
49	84826.5
50	87787.0
51	85756.0
52	83725.0
53	84243.0
54	84761.0
55	84954.5
56	85148.0
57	82190.5
58	79233.0
59	72834.5
60	66436.0
61	58743.5
62	51051.0
63	44135.5
64	37220.0
65	31679.0
66	26138.0
67	22338.5
68	18539.0
69	16205.0
70	13871.0
71	10786.5
72	7702.0
73	6752.5
74	5803.0
75	4290.0
76	2777.0
77	2441.5
78	2106.0
79	1763.0
80	1420.0
81	1157.5
82	895.0
83	707.5
84	520.0
85	424.5
86	329.0
87	218.5
88	108.0
89	73.0
90	38.0
91	28.0
92	18.0
93	14.5
94	11.0
95	11.5
96	12.0
97	10.5
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0338835810619619
2	0.005136606703542096
3	1.802318141593718E-4
4	0.0
5	1.802318141593718E-4
6	0.0
7	0.0
8	0.0
9	0.0
10	9.01159070796859E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.912749778765448E-4
17	0.0
18	2.7034772123905767E-4
19	0.0
20	3.604636283187436E-4
21	0.0
22	0.0
23	0.0
24	0.0011715067920359166
25	9.01159070796859E-5
26	3.604636283187436E-4
27	3.604636283187436E-4
28	0.00153197042035466
29	3.604636283187436E-4
30	9.912749778765448E-4
31	4.505795353984294E-4
32	4.505795353984294E-4
33	1.802318141593718E-4
34	7.209272566374872E-4
35	9.01159070796859E-5
36	9.01159070796859E-5
37	0.0013517386061952885
38	0.0
39	6.308113495578012E-4
40	0.0
41	0.0011715067920359166
42	0.00504649079646241
43	0.0038749840044264936
44	0.00351452037610775
45	0.002252897676992147
46	0.002343013584071833
47	9.912749778765448E-4
48	0.004325563539824923
49	0.004055215818585865
50	0.0024331294911515188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1109682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.32639747299181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.38713218995417	43.02611969553327
2	14.220875283838124	16.020213473026228
3	4.297889337999526	7.262538694412848
4	1.7920917123534779	4.03768280392306
5	0.9211560775462058	2.594270167926483
6	0.5415479649667669	1.8302067555244776
7	0.35584956042322774	1.4030606646691701
8	0.25496431766227357	1.1488977198460293
9	0.19003397743827047	0.9633536411905422
>10	0.9019337662005864	9.072545890526726
>50	0.06948610077450337	2.715750553020475
>100	0.059965978536865035	6.863274451579052
>500	0.005626832516115458	2.127658158963591
>1k	0.0014468997898582606	0.9344273298580736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1540	0.13877849690271626	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1184	0.1066972339823481	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1172	0.10561584309739186	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1133	0.10210132272128411	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1116	0.10056935230092945	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.011590707968589E-5
7	0.0	0.0	0.0	0.0	9.011590707968589E-5
8	0.0	0.0	0.0	0.0	1.8023181415937177E-4
9	0.0	0.0	0.0	0.0	1.8023181415937177E-4
10	0.0	0.0	0.0	0.0	2.7034772123905767E-4
11	0.0	0.0	0.0	0.0	2.7034772123905767E-4
12	0.0	0.0	0.0	0.0	6.308113495578012E-4
13	0.0	0.0	0.0	0.0	6.308113495578012E-4
14	0.0	0.0	0.0	0.0	6.308113495578012E-4
15	0.0	0.0	0.0	9.011590707968589E-5	8.110431637171731E-4
16	0.0	0.0	0.0	1.8023181415937177E-4	8.110431637171731E-4
17	0.0	0.0	0.0	1.8023181415937177E-4	8.110431637171731E-4
18	0.0	0.0	0.0	1.8023181415937177E-4	8.110431637171731E-4
19	0.0	0.0	0.0	2.7034772123905767E-4	8.110431637171731E-4
20	0.0	0.0	0.0	4.5057953539842947E-4	8.110431637171731E-4
21	0.0	0.0	0.0	7.209272566374871E-4	9.011590707968589E-4
22	0.0	0.0	0.0	8.110431637171731E-4	9.011590707968589E-4
23	0.0	0.0	0.0	0.0013517386061952883	9.011590707968589E-4
24	0.0	0.0	0.0	0.0019825499557530896	9.011590707968589E-4
25	0.0	0.0	0.0	0.002343013584071833	9.011590707968589E-4
26	0.0	0.0	0.0	0.002343013584071833	9.011590707968589E-4
27	0.0	0.0	9.011590707968589E-5	0.0028837090265499484	9.011590707968589E-4
28	0.0	0.0	9.011590707968589E-5	0.003874984004426493	9.011590707968589E-4
29	0.0	0.0	9.011590707968589E-5	0.005767418053099897	9.011590707968589E-4
30	0.0	0.0	9.011590707968589E-5	0.009732517964606077	9.011590707968589E-4
31	0.0	0.0	9.011590707968589E-5	0.015499936017705973	9.011590707968589E-4
32	0.0	0.0	9.011590707968589E-5	0.02279932449116053	9.011590707968589E-4
33	0.0	0.0	9.011590707968589E-5	0.03045917659293383	9.011590707968589E-4
34	0.0	0.0	9.011590707968589E-5	0.03983123092922117	9.912749778765448E-4
35	0.0	0.0	9.011590707968589E-5	0.05010444433630536	9.912749778765448E-4
36	0.0	0.0	9.011590707968589E-5	0.06740669849560504	9.912749778765448E-4
37	0.0	0.0	9.011590707968589E-5	0.0907467184292437	9.912749778765448E-4
38	0.0	0.0	9.011590707968589E-5	0.11868264962394633	9.912749778765448E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGC	40	7.033324E-4	27.498959	4
GTATCAA	1515	0.0	25.711266	1
GGTATCA	680	0.0	25.243439	1
TACCGTA	55	0.004484366	19.999243	7
CGAATTG	60	0.0074133477	18.33264	14
CCTATAT	75	0.0012907179	17.600126	2
GGCGTTA	340	0.0	17.472288	42
CGCAATA	280	0.0	17.285837	36
TAGCGGC	295	0.0	17.152666	30
ATCAACG	2280	0.0	17.0783	3
ATACGAA	285	0.0	16.984108	40
CTAGCGG	285	0.0	16.982578	29
AACGCAG	2310	0.0	16.856504	6
TCAACGC	2325	0.0	16.747751	4
CGCCCCT	250	0.0	16.719366	5
CAACGCA	2350	0.0	16.663198	5
GAGTATA	80	0.0019839287	16.505323	1
TACGAAT	295	0.0	16.408377	41
GCGCAAT	295	0.0	16.406158	35
GTATTAG	175	3.608875E-9	16.348131	1
>>END_MODULE