FastQCFastQC Report
Thu 2 Feb 2017
SRR4062231_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062231_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909775
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29000.15185034886308596No Hit
GCGCAAGACGGACCAGAGCGAAAGC22290.11671531986752366No Hit
GGGTAGGCACACGCTGAGCCAGTCA19360.10137319841342567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7550.014.5919191
GTATCAA19500.012.7118071
CTATTCC3100.012.5642734
CGGACCA4800.012.4701449
AAGACGG4850.012.3409385
CCTATTC2950.012.2367313
GCGTTAT1353.7289283E-1011.9595971
GGTTCTA2900.011.79318513
CGCAAGA4700.011.7216422
CGCGTGC3600.011.6124099
TCCTATT3300.011.513422
CGAACGA1001.9312029E-611.39948116
CGTTATT1251.8364517E-811.3982862
GTCCTAT3500.011.3967921
CGGTTCT3100.011.33908612
CGCCAGT3100.011.33819418
GTGTAGG2200.011.2241131
TCGCGTA1701.0913936E-1111.1777199
GTACTAA855.339384E-511.1733261
GCGCGTG3850.011.1051378
Produced by FastQC (version 0.11.5)