##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062231_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1909775
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199913078765825	32.0	32.0	32.0	32.0	32.0
2	30.697724077443677	32.0	32.0	32.0	32.0	32.0
3	30.714397769370738	32.0	32.0	32.0	32.0	32.0
4	30.72826589519708	32.0	32.0	32.0	32.0	32.0
5	30.629021219777197	32.0	32.0	32.0	32.0	32.0
6	34.28430469557932	36.0	36.0	36.0	32.0	36.0
7	34.20908588708094	36.0	36.0	36.0	32.0	36.0
8	34.15844693746646	36.0	36.0	36.0	32.0	36.0
9	34.3126096006074	36.0	36.0	36.0	32.0	36.0
10	33.96147033027451	36.0	36.0	36.0	32.0	36.0
11	34.28921679255409	36.0	36.0	36.0	32.0	36.0
12	34.09354714560616	36.0	36.0	36.0	32.0	36.0
13	34.20264533780157	36.0	36.0	36.0	32.0	36.0
14	34.0883140684112	36.0	36.0	36.0	32.0	36.0
15	34.02862955060151	36.0	36.0	36.0	32.0	36.0
16	34.039612519799455	36.0	36.0	36.0	32.0	36.0
17	33.95875168540797	36.0	36.0	36.0	32.0	36.0
18	33.977811522299746	36.0	36.0	36.0	32.0	36.0
19	33.98125695435326	36.0	36.0	36.0	32.0	36.0
20	33.97167415009621	36.0	36.0	36.0	32.0	36.0
21	33.950787920042934	36.0	36.0	36.0	32.0	36.0
22	33.920898011545866	36.0	36.0	36.0	32.0	36.0
23	33.88885915880143	36.0	36.0	36.0	32.0	36.0
24	33.856973203649645	36.0	36.0	36.0	32.0	36.0
25	33.492350145959605	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	35.0
5	169.0
6	391.0
7	83.0
8	323.0
9	243.0
10	147.0
11	48.0
12	75.0
13	97.0
14	287.0
15	467.0
16	743.0
17	930.0
18	1412.0
19	1816.0
20	2845.0
21	4130.0
22	6291.0
23	9343.0
24	13167.0
25	18614.0
26	24897.0
27	31760.0
28	41371.0
29	54327.0
30	69975.0
31	94364.0
32	131516.0
33	183753.0
34	392417.0
35	823737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.56979059705314	17.025358643978336	11.418484911478545	25.986365847489978
2	17.254511400045974	18.980213878822248	36.68147994552232	27.08379477560946
3	19.343256416002884	22.39977253638685	27.966190445271543	30.290780602338728
4	13.180541118003314	14.973357806620879	34.3433713961033	37.502729679272505
5	15.463073495772623	35.891725066693624	32.8900536010891	15.755147836444655
6	36.001787737129284	34.125240102737244	15.891683669031803	13.98128849110166
7	31.441970957304	29.709033134966294	19.6870857342944	19.161910173435313
8	28.83036742088279	32.76929625366152	18.404068615425263	19.996267710030423
9	26.934189052671094	14.426423567441635	18.160304779240033	40.479082600647246
10	16.750803000384852	26.265426887902926	30.416910039838047	26.56686007187417
11	37.7409501936201	21.0835384893699	21.352745158186448	19.822766158823548
12	24.66512830434616	23.27588331306827	27.935904715914184	24.123083666671384
13	29.527881064280905	19.054524787712158	24.78409450767466	26.633499640332282
14	23.768721013195467	19.11395198760294	24.328706628243886	32.7886203709577
15	25.484574418162016	26.779202270986254	21.461585611074728	26.274637699777003
16	26.372227013339693	25.62327487005004	22.712290920147947	25.292207196462318
17	24.63685449172211	25.97126711267668	24.29854605487695	25.09333234072425
18	25.399518873646404	24.88819762141783	25.067165301867732	24.64511820306803
19	25.962997983118573	24.851459408688946	24.594317509969095	24.591225098223383
20	26.107667997033534	24.131300134434312	24.204832821888946	25.5561990466432
21	26.81406926667844	24.043601288667762	24.053768912960418	25.08856053169338
22	26.455774429067212	23.785314910008864	24.408388934054372	25.35052172686955
23	24.972985096996865	24.009986343193553	25.023189320230628	25.993839239578953
24	25.16744139485666	24.59264531460219	24.698149509874572	25.541763780666578
25	25.31905560275317	24.198803701993604	24.588101064278614	25.894039630974614
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	247.0
1	247.0
2	406.5
3	566.0
4	566.0
5	566.0
6	1177.5
7	1789.0
8	1789.0
9	1789.0
10	2134.5
11	2480.0
12	2480.0
13	2480.0
14	3384.0
15	4288.0
16	4288.0
17	4288.0
18	7058.5
19	9829.0
20	9829.0
21	9829.0
22	15480.0
23	21131.0
24	21131.0
25	21131.0
26	31642.5
27	42154.0
28	42154.0
29	42154.0
30	53813.5
31	65473.0
32	65473.0
33	65473.0
34	84527.0
35	103581.0
36	103581.0
37	103581.0
38	124525.0
39	145469.0
40	145469.0
41	145469.0
42	169866.0
43	194263.0
44	194263.0
45	194263.0
46	218744.5
47	243226.0
48	243226.0
49	243226.0
50	255030.5
51	266835.0
52	266835.0
53	266835.0
54	253807.0
55	240779.0
56	240779.0
57	240779.0
58	223546.0
59	206313.0
60	206313.0
61	206313.0
62	181525.0
63	156737.0
64	156737.0
65	156737.0
66	128523.5
67	100310.0
68	100310.0
69	100310.0
70	76141.5
71	51973.0
72	51973.0
73	51973.0
74	39194.0
75	26415.0
76	26415.0
77	26415.0
78	20901.0
79	15387.0
80	15387.0
81	15387.0
82	10621.5
83	5856.0
84	5856.0
85	5856.0
86	4295.5
87	2735.0
88	2735.0
89	2735.0
90	1838.5
91	942.0
92	942.0
93	942.0
94	575.0
95	208.0
96	208.0
97	208.0
98	498.5
99	789.0
100	789.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.283462711575971E-4
2	1.0472437852626618E-4
3	9.948815959995288E-4
4	0.010996059745257948
5	0.03518739118482544
6	0.06372478433323298
7	0.10639996858268644
8	0.10990823526331638
9	0.12022358654815357
10	0.13368066918877877
11	0.14085428911782802
12	0.13362830699951567
13	0.12875862339804428
14	0.11655823329973426
15	0.13540862143446217
16	0.12095665719783744
17	0.12273697163278395
18	0.11352122632247255
19	0.09838855362542707
20	0.0932046968883769
21	0.09205272872458797
22	0.103467685983951
23	0.08215627495385583
24	0.09393776753806077
25	0.09016768991111519
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1909775.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.40333857984649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.65458493934844	40.169561682742774
2	13.187027209778911	13.820885034713854
3	4.252706709530587	6.685680887409487
4	1.859503020229649	3.8977666543736578
5	1.0415154216649332	2.7289442638819534
6	0.6374737770777982	2.0043452505588863
7	0.4238025525535256	1.5546068056725915
8	0.3186568445672468	1.335894601331436
9	0.22223368012211248	1.0481208104955924
>10	1.250167929055584	11.656431097547012
>50	0.07984254192935357	2.8858618724111422
>100	0.06114174872221456	6.814824389842626
>500	0.008131271318632127	2.9267153308462746
>1k	0.003212354101188001	2.4703613181727517
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2900	0.15185034886308596	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2229	0.11671531986752366	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1936	0.10137319841342567	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.236218926313309E-5
2	0.0	0.0	0.0	0.0	5.236218926313309E-5
3	0.0	0.0	0.0	0.0	5.236218926313309E-5
4	0.0	0.0	0.0	0.0	5.236218926313309E-5
5	0.0	0.0	0.0	0.0	5.236218926313309E-5
6	0.0	0.0	0.0	0.0	5.236218926313309E-5
7	0.0	0.0	0.0	0.0	5.236218926313309E-5
8	0.0	0.0	0.0	0.0	5.236218926313309E-5
9	0.0	0.0	0.0	5.236218926313309E-5	5.236218926313309E-5
10	0.0	0.0	0.0	5.236218926313309E-5	5.236218926313309E-5
11	0.0	0.0	0.0	5.236218926313309E-5	5.236218926313309E-5
12	0.0	0.0	0.0	5.236218926313309E-5	2.6181094631566544E-4
13	0.0	0.0	0.0	5.236218926313309E-5	2.6181094631566544E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	755	0.0	14.591919	1
GTATCAA	1950	0.0	12.711807	1
CTATTCC	310	0.0	12.564273	4
CGGACCA	480	0.0	12.470144	9
AAGACGG	485	0.0	12.340938	5
CCTATTC	295	0.0	12.236731	3
GCGTTAT	135	3.7289283E-10	11.959597	1
GGTTCTA	290	0.0	11.793185	13
CGCAAGA	470	0.0	11.721642	2
CGCGTGC	360	0.0	11.612409	9
TCCTATT	330	0.0	11.51342	2
CGAACGA	100	1.9312029E-6	11.399481	16
CGTTATT	125	1.8364517E-8	11.398286	2
GTCCTAT	350	0.0	11.396792	1
CGGTTCT	310	0.0	11.339086	12
CGCCAGT	310	0.0	11.338194	18
GTGTAGG	220	0.0	11.224113	1
TCGCGTA	170	1.0913936E-11	11.177719	9
GTACTAA	85	5.339384E-5	11.173326	1
GCGCGTG	385	0.0	11.105137	8
>>END_MODULE