FastQCFastQC Report
Thu 2 Feb 2017
SRR4062231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909775
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23050.12069484625152177No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19590.10257752876647773No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19460.101896820306057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9050.027.7213821
GTATCAA21100.027.5346831
TCAACGC31050.018.5630994
GTGTACG1203.153964E-718.3390731
TCTAGCG4100.018.24340228
ATCAACG32150.018.0648253
CAACGCA31950.018.0401955
TTAACGG1355.5322744E-817.92543435
AACGCAG32900.017.5192786
TATCAAC33650.017.194182
ACCGTCG5400.017.1106418
CAATACG4650.017.03268438
TAAACGC3500.016.97096428
GGCGTTA6150.016.81430842
ATACCGT5500.016.799546
TAACGGC1451.2503551E-716.68963436
CTAGCGG4400.016.4995529
TCATATA1351.0865624E-616.2958492
TAGCGGC4600.016.26042430
GGATAGT1501.8397805E-716.1328936
Produced by FastQC (version 0.11.5)