##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062231_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1909775
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.292925082797712	32.0	32.0	32.0	32.0	32.0
2	31.43309447447998	32.0	32.0	32.0	32.0	32.0
3	31.55915016166826	32.0	32.0	32.0	32.0	32.0
4	31.641927452186774	32.0	32.0	32.0	32.0	32.0
5	31.614201149350055	32.0	32.0	32.0	32.0	32.0
6	35.24894503279182	36.0	36.0	36.0	36.0	36.0
7	35.257052794177326	36.0	36.0	36.0	36.0	36.0
8	35.20732704114359	36.0	36.0	36.0	36.0	36.0
9	35.293309421266905	36.0	36.0	36.0	36.0	36.0
10	35.173193700828634	36.0	36.0	36.0	36.0	36.0
11	35.29444515715202	36.0	36.0	36.0	36.0	36.0
12	35.21059287088793	36.0	36.0	36.0	36.0	36.0
13	35.250211674150094	36.0	36.0	36.0	36.0	36.0
14	35.21561440483826	36.0	36.0	36.0	36.0	36.0
15	35.18946839287351	36.0	36.0	36.0	36.0	36.0
16	35.20233430639735	36.0	36.0	36.0	36.0	36.0
17	35.181228678771056	36.0	36.0	36.0	36.0	36.0
18	35.180414446728015	36.0	36.0	36.0	36.0	36.0
19	35.16506394732364	36.0	36.0	36.0	36.0	36.0
20	35.1720234058986	36.0	36.0	36.0	36.0	36.0
21	35.167548533204176	36.0	36.0	36.0	36.0	36.0
22	35.148974617428756	36.0	36.0	36.0	36.0	36.0
23	35.11986647641738	36.0	36.0	36.0	36.0	36.0
24	35.105951748242596	36.0	36.0	36.0	36.0	36.0
25	35.08884083203519	36.0	36.0	36.0	36.0	36.0
26	35.04617245487034	36.0	36.0	36.0	36.0	36.0
27	35.03411658441439	36.0	36.0	36.0	36.0	36.0
28	35.0171538532026	36.0	36.0	36.0	36.0	36.0
29	34.99787461873781	36.0	36.0	36.0	36.0	36.0
30	34.97608618816353	36.0	36.0	36.0	36.0	36.0
31	34.98365566624341	36.0	36.0	36.0	36.0	36.0
32	34.960136665313975	36.0	36.0	36.0	36.0	36.0
33	34.94100771033237	36.0	36.0	36.0	36.0	36.0
34	34.93111701640245	36.0	36.0	36.0	36.0	36.0
35	34.92227356625781	36.0	36.0	36.0	36.0	36.0
36	34.89509287743321	36.0	36.0	36.0	32.0	36.0
37	34.8889324658664	36.0	36.0	36.0	32.0	36.0
38	34.87149009700096	36.0	36.0	36.0	32.0	36.0
39	34.85792200651909	36.0	36.0	36.0	32.0	36.0
40	34.84792397010119	36.0	36.0	36.0	32.0	36.0
41	34.81671296356901	36.0	36.0	36.0	32.0	36.0
42	34.788561479755465	36.0	36.0	36.0	32.0	36.0
43	34.790298333573325	36.0	36.0	36.0	32.0	36.0
44	34.74377138668168	36.0	36.0	36.0	32.0	36.0
45	34.73044835124556	36.0	36.0	36.0	32.0	36.0
46	34.71783848882722	36.0	36.0	36.0	32.0	36.0
47	34.69295545286749	36.0	36.0	36.0	32.0	36.0
48	34.666803157440015	36.0	36.0	36.0	32.0	36.0
49	34.65804505766386	36.0	36.0	36.0	32.0	36.0
50	34.2456776321818	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	5.0
20	16.0
21	43.0
22	138.0
23	364.0
24	921.0
25	2182.0
26	4543.0
27	8711.0
28	14873.0
29	23381.0
30	35214.0
31	51712.0
32	79132.0
33	134703.0
34	313160.0
35	1240674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.3552721036769	17.48652026386446	11.774747245126397	26.383460387332242
2	16.696907002719275	19.403351624154777	36.41229700356234	27.48744436956361
3	18.73651999478472	22.768465152664742	28.22246413578996	30.272550716760577
4	12.92539697084735	15.350342317812308	34.465787854590204	37.25847285675014
5	15.065125472896021	36.16410310115066	32.7918210260371	15.97895039991622
6	36.11499173723355	34.25712598153078	15.77879453673004	13.849087744505628
7	31.560471783325262	29.57782984906599	19.76583628961527	19.09586207799348
8	28.802398188268253	32.87313950596275	18.378814258224136	19.945648047544868
9	27.14283096176251	14.16711392703329	18.105221819324267	40.58483329187993
10	16.72885559113863	26.234754724647757	30.499347566096898	26.537042118116716
11	38.04736160018851	20.94178633608671	21.300833867864018	19.71001819586077
12	24.77549699597963	23.201750573628082	27.982682767699213	24.04006966269307
13	29.695592412718774	18.913903470304096	24.919532405649882	26.470971711327252
14	23.952140959013494	18.880286944797163	24.383657760730976	32.78391433545836
15	25.49855349452161	26.692830307235145	21.584427484913142	26.224188713330104
16	26.411427984969905	25.54325970484373	22.761246392231936	25.284065917954436
17	24.719561204853974	25.91776518176225	24.25521331046851	25.107460302915264
18	25.528925779951166	24.728671089195696	25.131861635476998	24.610541495376136
19	26.048042308648924	24.705580500320718	24.640494299066646	24.605882891963713
20	26.20862848262197	23.995270629730943	24.259229393308505	25.53687149433858
21	26.922490869343246	23.901192548860468	24.214318440654004	24.96199814114228
22	26.482281944208086	23.60890680839366	24.56975298137215	25.339058266026104
23	25.01912528962836	23.74766660994096	25.214907515283215	26.018300585147465
24	25.307748899727976	24.41517367411003	24.766056378959604	25.51102104720239
25	25.448862717048275	23.98313724525087	24.750087837756464	25.81791219994439
26	25.200509173127767	24.858163028606167	25.004463904683462	24.936863893582604
27	25.684364306603168	24.328961940343447	24.56679224930816	25.419881503745223
28	25.450126954521014	24.060984249680192	25.060178380396486	25.428710415402318
29	25.135592528089827	24.53986509341982	25.05495437660949	25.269588001880862
30	25.14370960867211	24.612279906207814	25.152558915965095	25.091451569154984
31	25.66906102632469	24.221239670200433	24.40021447653796	25.70948482693691
32	25.601306552976872	24.26412142271429	24.172120237223773	25.962451787085065
33	25.084473478757403	24.176197041425386	24.7800914350464	25.95923804477081
34	25.928125150542165	24.184558929794466	24.82243879348443	25.064877126178942
35	26.140122412454254	24.05762360238625	24.93615733388209	24.866096651277402
36	25.054626968364268	24.60802892074495	24.595776142794083	25.741567968096696
37	26.140100453254465	24.230368049330174	24.52794720130028	25.10158429611508
38	25.302980717623804	24.0093728318781	24.990849707426268	25.696796743071825
39	25.966743499975394	24.102951048350526	24.25742055816379	25.67288489351029
40	25.966816981294098	24.297507765324866	24.675196829778635	25.0604784236024
41	25.107539859783206	24.76969583041193	25.010930710032742	25.111833599772115
42	26.207083258713737	24.600012776969617	24.497482832256193	24.695421132060456
43	25.74469578039761	23.832464264132806	24.741714169615637	25.681125785853947
44	25.38608239217132	24.3670720365964	24.49143738060927	25.755408190623008
45	25.373414119072567	24.685939416979146	24.44077553890759	25.499870925040703
46	25.29192638951725	24.446832219038523	24.580306724308386	25.68093466713584
47	25.440344672664644	24.443933616580985	24.911952010573092	25.203769700181276
48	26.187543789646323	24.83633537868291	23.908544701832437	25.067576129838333
49	25.41787648942381	24.934924844806687	24.116478113226318	25.53072055254318
50	25.51082816422323	25.002644336250658	24.16164068235395	25.324886817172164
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	59.0
2	49.0
3	165.5
4	282.0
5	297.5
6	313.0
7	256.0
8	199.0
9	248.5
10	298.0
11	520.0
12	742.0
13	1434.5
14	2127.0
15	2653.0
16	3179.0
17	3045.5
18	2912.0
19	2717.0
20	2522.0
21	3033.0
22	3544.0
23	4371.0
24	5198.0
25	6814.0
26	8430.0
27	10692.5
28	12955.0
29	15068.0
30	17181.0
31	20037.5
32	22894.0
33	26780.5
34	30667.0
35	36331.0
36	41995.0
37	50978.0
38	59961.0
39	67545.5
40	75130.0
41	83431.0
42	91732.0
43	96999.5
44	102267.0
45	112586.0
46	122905.0
47	131537.5
48	140170.0
49	147196.0
50	154222.0
51	151510.5
52	148799.0
53	149601.5
54	150404.0
55	150788.5
56	151173.0
57	146012.5
58	140852.0
59	130146.5
60	119441.0
61	104924.5
62	90408.0
63	78008.0
64	65608.0
65	55096.5
66	44585.0
67	38393.5
68	32202.0
69	28370.5
70	24539.0
71	19275.0
72	14011.0
73	12222.0
74	10433.0
75	7765.5
76	5098.0
77	4568.0
78	4038.0
79	3437.5
80	2837.0
81	2182.0
82	1527.0
83	1271.0
84	1015.0
85	781.0
86	547.0
87	367.0
88	187.0
89	118.5
90	50.0
91	36.5
92	23.0
93	19.0
94	15.0
95	16.5
96	18.0
97	17.0
98	16.0
99	11.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03209802201830059
2	0.0038748020054718488
3	1.0472437852626618E-4
4	0.0
5	0.0
6	1.5708656778939928E-4
7	0.0
8	0.0
9	0.0
10	1.5708656778939928E-4
11	0.0
12	5.236218926313309E-5
13	0.0
14	0.0
15	0.0
16	3.6653532484193165E-4
17	0.0
18	4.188975141050647E-4
19	0.0
20	3.6653532484193165E-4
21	0.0
22	0.0
23	0.0
24	0.0010472437852626618
25	2.0944875705253236E-4
26	6.283462711575971E-4
27	5.236218926313309E-4
28	0.0018850388134727914
29	4.7125970336819785E-4
30	0.001099605974525795
31	4.7125970336819785E-4
32	8.901572174732625E-4
33	2.0944875705253236E-4
34	5.75984081894464E-4
35	1.0472437852626618E-4
36	2.0944875705253236E-4
37	0.001623227867157126
38	0.0
39	6.807084604207302E-4
40	1.5708656778939928E-4
41	9.425194067363957E-4
42	0.004660234844418845
43	0.0036129910591561837
44	0.004136612951787514
45	0.001675590056420259
46	0.0024610228953672554
47	0.001099605974525795
48	0.004031888573261248
49	0.003141731355787986
50	0.001727952245683392
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1909775.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.16914630541961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16124115360203	40.49428173698211
2	13.749942539427524	14.621454131398695
3	4.254825153580811	6.786762632841527
4	1.8292756167355022	3.890440915965865
5	1.0168797101476228	2.703331304192582
6	0.6262838891172415	1.9979387837521085
7	0.4377000136540111	1.6290495244698
8	0.30672336935419225	1.3046575760387444
9	0.2396982251589223	1.1470095002361735
>10	1.23672258943319	11.583102995179386
>50	0.07358003715971424	2.707267564596032
>100	0.05681678085925701	6.271491715582997
>500	0.007435760891758097	2.6953590133067546
>1k	0.0028751608781464643	2.1678526054572536
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2305	0.12069484625152177	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1959	0.10257752876647773	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1946	0.101896820306057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5708656778939928E-4
2	0.0	0.0	0.0	0.0	1.5708656778939928E-4
3	0.0	0.0	0.0	0.0	1.5708656778939928E-4
4	0.0	0.0	0.0	0.0	1.5708656778939928E-4
5	0.0	0.0	0.0	0.0	1.5708656778939928E-4
6	0.0	0.0	0.0	0.0	1.5708656778939928E-4
7	0.0	0.0	0.0	0.0	1.5708656778939928E-4
8	0.0	0.0	0.0	0.0	1.5708656778939928E-4
9	0.0	0.0	0.0	0.0	1.5708656778939928E-4
10	0.0	0.0	0.0	0.0	1.5708656778939928E-4
11	0.0	0.0	0.0	0.0	1.5708656778939928E-4
12	0.0	0.0	0.0	0.0	6.807084604207302E-4
13	0.0	0.0	0.0	0.0	8.901572174732625E-4
14	0.0	0.0	0.0	0.0	8.901572174732625E-4
15	0.0	0.0	0.0	1.0472437852626618E-4	0.0010472437852626618
16	0.0	0.0	0.0	1.0472437852626618E-4	0.001099605974525795
17	0.0	0.0	0.0	1.5708656778939928E-4	0.001099605974525795
18	0.0	0.0	0.0	2.6181094631566544E-4	0.001099605974525795
19	0.0	0.0	0.0	2.6181094631566544E-4	0.001099605974525795
20	0.0	0.0	0.0	4.712597033681978E-4	0.001099605974525795
21	0.0	0.0	0.0	6.283462711575971E-4	0.001099605974525795
22	0.0	0.0	0.0	7.854328389469964E-4	0.001099605974525795
23	0.0	5.236218926313309E-5	0.0	0.001099605974525795	0.001204330353052061
24	0.0	5.236218926313309E-5	0.0	0.0013090547315783272	0.001204330353052061
25	0.0	5.236218926313309E-5	0.0	0.0014137791101045934	0.001204330353052061
26	0.0	5.236218926313309E-5	0.0	0.001623227867157126	0.0013090547315783272
27	0.0	5.236218926313309E-5	0.0	0.0019374010027359244	0.0013090547315783272
28	0.0	5.236218926313309E-5	0.0	0.002408660706104122	0.0013090547315783272
29	0.0	5.236218926313309E-5	0.0	0.0032988179235773846	0.0013090547315783272
30	0.0	5.236218926313309E-5	0.0	0.005131494547787043	0.0013090547315783272
31	0.0	5.236218926313309E-5	0.0	0.008377950282101294	0.0013090547315783272
32	0.0	5.236218926313309E-5	0.0	0.012671649801678208	0.0013090547315783272
33	0.0	5.236218926313309E-5	0.0	0.01665117618567632	0.0013614169208414604
34	0.0	5.236218926313309E-5	0.0	0.021416135408621435	0.0014661412993677266
35	0.0	5.236218926313309E-5	0.0	0.026338181199355944	0.0014661412993677266
36	0.0	5.236218926313309E-5	0.0	0.03246455734314252	0.0014661412993677266
37	0.0	5.236218926313309E-5	0.0	0.04236101111387467	0.0014661412993677266
38	0.0	5.236218926313309E-5	0.0	0.05618462907934181	0.0014661412993677266
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	905	0.0	27.721382	1
GTATCAA	2110	0.0	27.534683	1
TCAACGC	3105	0.0	18.563099	4
GTGTACG	120	3.153964E-7	18.339073	1
TCTAGCG	410	0.0	18.243402	28
ATCAACG	3215	0.0	18.064825	3
CAACGCA	3195	0.0	18.040195	5
TTAACGG	135	5.5322744E-8	17.925434	35
AACGCAG	3290	0.0	17.519278	6
TATCAAC	3365	0.0	17.19418	2
ACCGTCG	540	0.0	17.110641	8
CAATACG	465	0.0	17.032684	38
TAAACGC	350	0.0	16.970964	28
GGCGTTA	615	0.0	16.814308	42
ATACCGT	550	0.0	16.79954	6
TAACGGC	145	1.2503551E-7	16.689634	36
CTAGCGG	440	0.0	16.49955	29
TCATATA	135	1.0865624E-6	16.295849	2
TAGCGGC	460	0.0	16.260424	30
GGATAGT	150	1.8397805E-7	16.132893	6
>>END_MODULE