FastQCFastQC Report
Thu 2 Feb 2017
SRR4062230_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062230_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186999
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20790.17514757805187706No Hit
TATCAACGCAGAGTACTTTTTTTTT13580.11440616209449207No Hit
GCGCAAGACGGACCAGAGCGAAAGC13210.11128905753079825No Hit
GAATAGGACCGCGGTTCTATTTTGT11960.1007582988696705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1350.016.1876310
CCGTCGT1400.014.9308269
CGTCTTA653.369636E-614.61574215
CCCGTAG400.00527431314.25155214
TAACGAA759.65525E-713.93485113
GGTATCA6850.013.5852661
CGCGTAA1351.8189894E-1213.3723910
CGATAAC655.4426353E-513.15583310
TCGCGTA1450.013.1054289
ATAACGA801.995817E-613.06392212
GGGTCGC1700.012.8537996
CGCCAGT1700.012.85271418
CGTTATT751.4826464E-512.6627022
GTCGCGT1600.012.4701078
GCGTAAC1457.2759576E-1212.45015611
CGAACGA701.09022985E-412.21458416
GCGTTAT701.09496425E-412.2089171
TCGCCAG1950.012.17978417
ATCGCCA1950.012.17978416
GTCGTAG1800.012.14072211
Produced by FastQC (version 0.11.5)