##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062230_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1186999
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238886469154565	32.0	32.0	32.0	32.0	32.0
2	30.73838225642987	32.0	32.0	32.0	32.0	32.0
3	30.761639226317797	32.0	32.0	32.0	32.0	32.0
4	30.76076812196135	32.0	32.0	32.0	32.0	32.0
5	30.663355234503147	32.0	32.0	32.0	32.0	32.0
6	34.33099438162964	36.0	36.0	36.0	32.0	36.0
7	34.26420915266146	36.0	36.0	36.0	32.0	36.0
8	34.21543657576797	36.0	36.0	36.0	32.0	36.0
9	34.362966607385516	36.0	36.0	36.0	32.0	36.0
10	34.015056457503334	36.0	36.0	36.0	32.0	36.0
11	34.356576543029945	36.0	36.0	36.0	32.0	36.0
12	34.14521916193695	36.0	36.0	36.0	32.0	36.0
13	34.25452843683946	36.0	36.0	36.0	32.0	36.0
14	34.1428788061321	36.0	36.0	36.0	32.0	36.0
15	34.09456452785554	36.0	36.0	36.0	32.0	36.0
16	34.08823933297332	36.0	36.0	36.0	32.0	36.0
17	34.01306403796465	36.0	36.0	36.0	32.0	36.0
18	34.019469266612695	36.0	36.0	36.0	32.0	36.0
19	34.03791494348352	36.0	36.0	36.0	32.0	36.0
20	34.02824265226845	36.0	36.0	36.0	32.0	36.0
21	34.011381643960945	36.0	36.0	36.0	32.0	36.0
22	33.9814085774293	36.0	36.0	36.0	32.0	36.0
23	33.94151300885679	36.0	36.0	36.0	32.0	36.0
24	33.91618611304643	36.0	36.0	36.0	32.0	36.0
25	33.54903584586002	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	15.0
5	93.0
6	257.0
7	62.0
8	220.0
9	143.0
10	82.0
11	23.0
12	43.0
13	58.0
14	178.0
15	270.0
16	465.0
17	593.0
18	788.0
19	1112.0
20	1718.0
21	2452.0
22	3680.0
23	5428.0
24	7672.0
25	10743.0
26	14644.0
27	18936.0
28	24532.0
29	32725.0
30	42410.0
31	57452.0
32	81378.0
33	113794.0
34	246530.0
35	518501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.112166256103684	17.419131448675135	12.070130447822557	25.398571847398628
2	16.91305937519482	19.077013026182104	37.2728084556451	26.737119142977978
3	19.533139622558853	22.387663617224366	27.996037044868842	30.08315971534794
4	13.15134281200632	15.269341758820431	34.64880463401791	36.93051079515535
5	15.496986327229578	35.736293519592245	32.94476955853402	15.821950594644157
6	35.359972079307575	34.24955130469383	16.316829045613073	14.07364757038552
7	31.380337804045993	29.870787042776648	19.419136269447094	19.329738883730265
8	28.64862266318291	32.81344090465162	18.55588372716738	19.982052704998086
9	26.891635205484537	14.873041899884276	18.598956804556053	39.636366090075136
10	17.067255374035575	26.389420813853633	30.064652081705923	26.47867173040487
11	37.21725221288213	21.44504661984997	21.504186168938322	19.833514998329584
12	24.857160933842295	23.42822194571567	27.861484277790666	23.85313284265137
13	29.32005273773101	19.28453153087038	24.907443257592437	26.487972473806167
14	23.806913118325642	19.52773319185797	24.544176329136025	32.12117736068036
15	25.29947191712642	26.70698992762059	21.912486713569873	26.08105144168312
16	26.13168238330606	25.906729195836636	22.989507245398876	24.972081175458424
17	24.31650246305419	26.015925501045956	24.613165530737565	25.05440650516229
18	25.06184324498022	25.027094636822223	25.42695665448497	24.484105463712584
19	25.82605652509392	24.982270026858487	24.994666250648272	24.197007197399323
20	26.03154766723333	24.35440783615316	24.534020076094876	25.080024420518633
21	26.767854357184117	24.17863000299347	24.312956662155383	24.740558977667035
22	26.238345184925315	24.011870707452335	24.712002941533644	25.037781166088706
23	24.76478191474562	24.19599216543974	25.21789132962403	25.82133459019061
24	24.824923919376886	24.83183842322329	24.94112131328352	25.402116344116305
25	25.109554538639845	24.386414478316336	25.066887925938858	25.43714305710496
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	318.0
1	318.0
2	353.5
3	389.0
4	389.0
5	389.0
6	772.0
7	1155.0
8	1155.0
9	1155.0
10	1369.0
11	1583.0
12	1583.0
13	1583.0
14	2213.5
15	2844.0
16	2844.0
17	2844.0
18	4632.0
19	6420.0
20	6420.0
21	6420.0
22	10387.0
23	14354.0
24	14354.0
25	14354.0
26	21312.0
27	28270.0
28	28270.0
29	28270.0
30	36019.0
31	43768.0
32	43768.0
33	43768.0
34	55524.5
35	67281.0
36	67281.0
37	67281.0
38	79688.5
39	92096.0
40	92096.0
41	92096.0
42	108058.5
43	124021.0
44	124021.0
45	124021.0
46	138600.5
47	153180.0
48	153180.0
49	153180.0
50	159677.5
51	166175.0
52	166175.0
53	166175.0
54	156479.0
55	146783.0
56	146783.0
57	146783.0
58	135409.5
59	124036.0
60	124036.0
61	124036.0
62	108692.0
63	93348.0
64	93348.0
65	93348.0
66	76515.0
67	59682.0
68	59682.0
69	59682.0
70	45270.0
71	30858.0
72	30858.0
73	30858.0
74	23004.0
75	15150.0
76	15150.0
77	15150.0
78	12088.0
79	9026.0
80	9026.0
81	9026.0
82	6219.5
83	3413.0
84	3413.0
85	3413.0
86	2479.0
87	1545.0
88	1545.0
89	1545.0
90	1083.5
91	622.0
92	622.0
93	622.0
94	383.5
95	145.0
96	145.0
97	145.0
98	341.0
99	537.0
100	537.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.267067621792436E-4
2	4.212303464451107E-4
3	5.054764157341328E-4
4	0.010446512591838746
5	0.034288150200632014
6	0.06588042618401532
7	0.10800346082852638
8	0.1099411204221739
9	0.11887120376681025
10	0.1328560512687879
11	0.14043819750479994
12	0.13260331306092088
13	0.12721156462642344
14	0.11777600486605296
15	0.13302454340736597
16	0.11954517232112244
17	0.12459993647846376
18	0.11288973284728968
19	0.09721996395953156
20	0.0935973829801036
21	0.09132273910930001
22	0.10303294274047409
23	0.08188717934892953
24	0.09149123124787806
25	0.08930083344636348
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1186999.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.55920503998897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52375757375964	44.97800510164647
2	12.140620774664859	13.733277194140388
3	3.5787620675527383	6.072358127041505
4	1.4594025009875322	3.301705811569061
5	0.8299636265615619	2.3471041465214104
6	0.5079809403603986	1.7238598897350115
7	0.3554639281439925	1.4073330037351184
8	0.251203463470234	1.1366294557734673
9	0.18863719578820393	0.9602252851278211
>10	1.0109533251867016	10.372410540575833
>50	0.08107643329703601	3.1825190120103124
>100	0.06454106587054895	7.374211576539209
>500	0.0055406443372010995	2.0096618085061717
>1k	0.0020964600194814977	1.4006990470783491
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2079	0.17514757805187706	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1358	0.11440616209449207	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1321	0.11128905753079825	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1196	0.1007582988696705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.89722485023155E-4
2	0.0	0.0	0.0	0.0	5.89722485023155E-4
3	0.0	0.0	0.0	0.0	5.89722485023155E-4
4	0.0	0.0	0.0	0.0	5.89722485023155E-4
5	0.0	0.0	0.0	0.0	5.89722485023155E-4
6	0.0	0.0	0.0	0.0	6.739685543121772E-4
7	0.0	0.0	0.0	8.424606928902214E-5	6.739685543121772E-4
8	0.0	0.0	0.0	8.424606928902214E-5	6.739685543121772E-4
9	0.0	0.0	0.0	8.424606928902214E-5	6.739685543121772E-4
10	0.0	0.0	0.0	8.424606928902214E-5	8.424606928902215E-4
11	0.0	0.0	0.0	8.424606928902214E-5	8.424606928902215E-4
12	0.0	0.0	0.0	8.424606928902214E-5	0.0013479371086243543
13	0.0	0.0	0.0	8.424606928902214E-5	0.0015164292472023987
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	135	0.0	16.18763	10
CCGTCGT	140	0.0	14.930826	9
CGTCTTA	65	3.369636E-6	14.615742	15
CCCGTAG	40	0.005274313	14.251552	14
TAACGAA	75	9.65525E-7	13.934851	13
GGTATCA	685	0.0	13.585266	1
CGCGTAA	135	1.8189894E-12	13.37239	10
CGATAAC	65	5.4426353E-5	13.155833	10
TCGCGTA	145	0.0	13.105428	9
ATAACGA	80	1.995817E-6	13.063922	12
GGGTCGC	170	0.0	12.853799	6
CGCCAGT	170	0.0	12.852714	18
CGTTATT	75	1.4826464E-5	12.662702	2
GTCGCGT	160	0.0	12.470107	8
GCGTAAC	145	7.2759576E-12	12.450156	11
CGAACGA	70	1.09022985E-4	12.214584	16
GCGTTAT	70	1.09496425E-4	12.208917	1
TCGCCAG	195	0.0	12.179784	17
ATCGCCA	195	0.0	12.179784	16
GTCGTAG	180	0.0	12.140722	11
>>END_MODULE