FastQCFastQC Report
Thu 2 Feb 2017
SRR4062230_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062230_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186999
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13510.11381643960946893No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12100.10193774383971681No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11980.10092679100824854No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA407.03434E-427.49847429
GTATCAA17100.025.8636591
GGTATCA8200.025.2234251
ATCGATT901.5133992E-824.44308929
CGGATCG904.0821942E-721.99970426
ATACGAA2700.020.37353140
GTCTAGC1301.6207196E-920.3108431
TACGCAG654.933938E-420.3065645
ACCGTAT550.004485006419.998898
CGAGCCG2900.019.72304315
CGAATGC2700.019.55941443
CGCAATA2750.019.20055236
GTCTTAG2000.018.7029021
GGGTACG600.007405396618.3361781
ATACCGT3250.018.2759086
GCGCAAT2900.018.20665435
TAGGACG851.4314137E-418.1166424
CAATACG3050.018.03558538
AATACGA3050.018.03558539
TACGAAT3200.017.87777341
Produced by FastQC (version 0.11.5)