##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062230_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1186999
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31394634704831	32.0	32.0	32.0	32.0	32.0
2	31.478444379481363	32.0	32.0	32.0	32.0	32.0
3	31.59094573794923	32.0	32.0	32.0	32.0	32.0
4	31.661586067048077	32.0	32.0	32.0	32.0	32.0
5	31.638785710855696	32.0	32.0	32.0	32.0	32.0
6	35.29394380281702	36.0	36.0	36.0	36.0	36.0
7	35.29702215418884	36.0	36.0	36.0	36.0	36.0
8	35.24722261771071	36.0	36.0	36.0	36.0	36.0
9	35.323010381643115	36.0	36.0	36.0	36.0	36.0
10	35.21768847320006	36.0	36.0	36.0	36.0	36.0
11	35.333671721711646	36.0	36.0	36.0	36.0	36.0
12	35.2546657579324	36.0	36.0	36.0	36.0	36.0
13	35.286894091738915	36.0	36.0	36.0	36.0	36.0
14	35.247913435478885	36.0	36.0	36.0	36.0	36.0
15	35.23189910016773	36.0	36.0	36.0	36.0	36.0
16	35.236939542493296	36.0	36.0	36.0	36.0	36.0
17	35.222810634212834	36.0	36.0	36.0	36.0	36.0
18	35.21629335829264	36.0	36.0	36.0	36.0	36.0
19	35.21293446751008	36.0	36.0	36.0	36.0	36.0
20	35.20994710189309	36.0	36.0	36.0	36.0	36.0
21	35.19949974684056	36.0	36.0	36.0	36.0	36.0
22	35.18121919226554	36.0	36.0	36.0	36.0	36.0
23	35.15633543077964	36.0	36.0	36.0	36.0	36.0
24	35.13718798415163	36.0	36.0	36.0	36.0	36.0
25	35.12416017199678	36.0	36.0	36.0	36.0	36.0
26	35.08329071886328	36.0	36.0	36.0	36.0	36.0
27	35.06664622295385	36.0	36.0	36.0	36.0	36.0
28	35.051329445096414	36.0	36.0	36.0	36.0	36.0
29	35.0327632963465	36.0	36.0	36.0	36.0	36.0
30	35.01553918748036	36.0	36.0	36.0	36.0	36.0
31	35.024337846956904	36.0	36.0	36.0	36.0	36.0
32	34.995899743807705	36.0	36.0	36.0	36.0	36.0
33	34.97338413932952	36.0	36.0	36.0	36.0	36.0
34	34.960764920610714	36.0	36.0	36.0	36.0	36.0
35	34.95378092146666	36.0	36.0	36.0	36.0	36.0
36	34.92449783024249	36.0	36.0	36.0	36.0	36.0
37	34.917571960886235	36.0	36.0	36.0	36.0	36.0
38	34.900000758214624	36.0	36.0	36.0	32.0	36.0
39	34.886968733756305	36.0	36.0	36.0	32.0	36.0
40	34.876202086101166	36.0	36.0	36.0	32.0	36.0
41	34.84677998886267	36.0	36.0	36.0	32.0	36.0
42	34.816924866828025	36.0	36.0	36.0	32.0	36.0
43	34.82068729628247	36.0	36.0	36.0	32.0	36.0
44	34.768218001868576	36.0	36.0	36.0	32.0	36.0
45	34.7626105834967	36.0	36.0	36.0	32.0	36.0
46	34.74261983371511	36.0	36.0	36.0	32.0	36.0
47	34.71252713776507	36.0	36.0	36.0	32.0	36.0
48	34.68685651799201	36.0	36.0	36.0	32.0	36.0
49	34.67903848276199	36.0	36.0	36.0	32.0	36.0
50	34.27526813417703	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	8.0
21	39.0
22	85.0
23	216.0
24	567.0
25	1246.0
26	2762.0
27	5056.0
28	8760.0
29	13924.0
30	21058.0
31	31227.0
32	47373.0
33	81144.0
34	187002.0
35	786525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93831244074581	17.909196249868323	12.4771515853787	25.675339724007163
2	16.427987699566117	19.557942626058384	36.86237836471629	27.15169130965921
3	18.87029295752815	22.803661000271273	28.248320553193857	30.077725489006724
4	12.982150785299734	15.700687195187191	34.687897799408425	36.62926422010465
5	15.001529066157596	36.31452090524087	32.666750351095494	16.017199677506046
6	35.58549678515104	34.33443527841805	16.154447544718078	13.925620391712833
7	31.432545436011317	29.90760733581073	19.366402162091124	19.29344506608683
8	28.770959368963243	32.88873874367207	18.432281745814443	19.908020141550246
9	27.07087369071078	14.639776444630535	18.55839811154011	39.730951753118575
10	17.065150910111054	26.424391616498088	30.097523331968546	26.412934141422312
11	37.48023376599306	21.332284188950453	21.406252237786216	19.781229807270268
12	24.888647757917234	23.44593382134273	27.823865058016057	23.84155336272398
13	29.61139815619053	19.17448961625073	24.942986472608656	26.271125754950088
14	23.917627563291965	19.374237046535	24.55991959555147	32.148215794621564
15	25.41762882698301	26.77306383577408	21.846522195890643	25.962785141352267
16	26.12071383980812	25.879178324314715	23.049666003644514	24.95044183223265
17	24.464216060839142	25.964133078460893	24.582918772467373	24.98873208823259
18	25.160067936201973	24.912046881371126	25.548611789761715	24.379273392665183
19	25.964133078460893	24.866154057417067	24.96379525172304	24.205917612399
20	26.152174187759847	24.094625503898502	24.68713010585554	25.066070202486113
21	26.856383198300925	24.077863587079687	24.402632184188867	24.663121030430524
22	26.339280825004906	23.883507905229912	24.784603862345293	24.99260740741989
23	24.797788031477754	24.014719498027375	25.28902768920225	25.898464781292624
24	24.991617424299864	24.56271598822564	25.14907505227526	25.296591535199237
25	25.14576676138733	24.18763621536328	25.24298672534686	25.423610297902528
26	24.874093719092095	25.069882408841153	25.379656510479414	24.67636736158734
27	25.444294953719186	24.54631156207324	24.814383909593403	25.195009574614176
28	25.04781065128638	24.479727441376138	25.328187475041574	25.144274432295905
29	24.839362724738436	24.77693596665855	25.292778125529175	25.090923183073837
30	25.03178215094184	24.73043091457615	25.370960254830088	24.866826679651926
31	25.469106336947796	24.51989947691263	24.61998448176945	25.391009704370127
32	25.510704404762308	24.484156537387065	24.519203226991344	25.485935830859287
33	24.89757784126187	24.33237096240182	24.953096000923335	25.816955195412973
34	25.530457609726753	24.531042279910427	25.103749471353687	24.834750639009126
35	25.84798238238778	24.13938065398814	25.34241959100046	24.67021737262362
36	24.909014015211508	24.74827210875184	24.88154972721054	25.46116414882611
37	25.801799008750727	24.552838405138388	24.801451068967058	24.843911517143827
38	25.08601545413801	24.370343286750757	25.055181314848575	25.488459944262658
39	25.736844632670785	24.32865734839556	24.67828207048603	25.256215948447625
40	25.85486592659303	24.515184932759	24.890332679303015	24.739616461344955
41	24.828241589840495	24.92765295660357	25.314936052374627	24.929169401181312
42	26.06634072858367	24.80418018540221	24.762391811500734	24.367087274513388
43	25.622264009786377	24.014032533640677	25.087703335254215	25.276000121318738
44	25.06002780235056	24.717890391339147	24.810312144572222	25.41176966173807
45	25.274393839828807	24.779693002409473	24.750543395844918	25.195369761916798
46	25.07925622571027	24.800732619865826	24.807640948867412	25.31237020555649
47	25.32826840417663	24.605766201117792	25.114449538579226	24.951515856126356
48	26.049619446077944	25.04747445983585	24.117278344400876	24.78562774968533
49	25.252641839594865	25.09551736078055	24.38993156449644	25.26190923512815
50	25.356870859253366	25.24086255585585	24.306814582167917	25.09545200272287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	106.0
1	70.5
2	35.0
3	112.5
4	190.0
5	188.0
6	186.0
7	153.0
8	120.0
9	164.0
10	208.0
11	362.0
12	516.0
13	991.5
14	1467.0
15	1881.5
16	2296.0
17	2202.0
18	2108.0
19	2034.0
20	1960.0
21	2262.5
22	2565.0
23	3157.5
24	3750.0
25	4721.5
26	5693.0
27	7319.5
28	8946.0
29	10487.0
30	12028.0
31	13954.5
32	15881.0
33	18298.0
34	20715.0
35	24165.0
36	27615.0
37	33214.5
38	38814.0
39	43766.0
40	48718.0
41	53549.5
42	58381.0
43	61734.5
44	65088.0
45	71604.5
46	78121.0
47	83216.5
48	88312.0
49	92174.0
50	96036.0
51	94492.5
52	92949.0
53	92690.5
54	92432.0
55	92704.5
56	92977.0
57	89447.5
58	85918.0
59	78420.0
60	70922.0
61	62166.5
62	53411.0
63	45545.0
64	37679.0
65	31883.0
66	26087.0
67	22283.5
68	18480.0
69	16176.0
70	13872.0
71	10825.0
72	7778.0
73	6897.0
74	6016.0
75	4377.5
76	2739.0
77	2466.5
78	2194.0
79	1893.0
80	1592.0
81	1249.5
82	907.0
83	765.0
84	623.0
85	478.0
86	333.0
87	228.5
88	124.0
89	80.5
90	37.0
91	24.5
92	12.0
93	14.0
94	16.0
95	21.5
96	27.0
97	21.0
98	15.0
99	9.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031508029914094286
2	0.004128057395162085
3	8.424606928902214E-5
4	0.0
5	0.0
6	5.89722485023155E-4
7	0.0
8	0.0
9	0.0
10	8.424606928902214E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.424606928902214E-4
17	0.0
18	2.527382078670664E-4
19	0.0
20	3.369842771560886E-4
21	0.0
22	0.0
23	1.684921385780443E-4
24	0.001095198900757288
25	0.0
26	4.212303464451107E-4
27	5.054764157341328E-4
28	0.0021061517322255537
29	6.739685543121772E-4
30	0.0010109528314682656
31	6.739685543121772E-4
32	9.267067621792436E-4
33	0.0
34	4.212303464451107E-4
35	4.212303464451107E-4
36	2.527382078670664E-4
37	0.0010109528314682656
38	2.527382078670664E-4
39	0.0010109528314682656
40	0.0
41	0.0010109528314682656
42	0.005560240573075461
43	0.0036225809794279526
44	0.004128057395162085
45	0.0016006753164914207
46	0.002190397801514576
47	0.001095198900757288
48	0.0037910731180059967
49	0.00353833491013893
50	0.0012636910393353322
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1186999.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.92519382694422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24476032491822	45.902681015906275
2	12.510547543324247	14.493517826565158
3	3.572535833572964	6.208194918402531
4	1.4781532290763053	3.424892492006739
5	0.8285462567651442	2.3996851258855036
6	0.5143954392915681	1.7877873314796122
7	0.33433289675081546	1.3556408492910301
8	0.2457849921358412	1.138971464737805
9	0.18971277961999083	0.989023457784269
>10	0.9433021132318297	9.834985763807593
>50	0.07595651166076929	3.064499968134179
>100	0.055818828463795114	6.6400920354407145
>500	0.00483469736239117	1.8888152275061207
>1k	0.0013185538261066827	0.8712125230524256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1351	0.11381643960946893	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1210	0.10193774383971681	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1198	0.10092679100824854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.424606928902214E-5
2	0.0	0.0	0.0	0.0	8.424606928902214E-5
3	0.0	0.0	0.0	0.0	8.424606928902214E-5
4	0.0	0.0	0.0	0.0	8.424606928902214E-5
5	0.0	0.0	0.0	0.0	8.424606928902214E-5
6	0.0	0.0	0.0	0.0	8.424606928902214E-5
7	0.0	0.0	0.0	0.0	8.424606928902214E-5
8	0.0	0.0	0.0	0.0	8.424606928902214E-5
9	0.0	0.0	0.0	0.0	8.424606928902214E-5
10	0.0	0.0	0.0	0.0	8.424606928902214E-5
11	0.0	0.0	0.0	0.0	8.424606928902214E-5
12	0.0	0.0	0.0	0.0	4.2123034644511075E-4
13	0.0	0.0	0.0	0.0	6.739685543121772E-4
14	0.0	0.0	0.0	8.424606928902214E-5	6.739685543121772E-4
15	0.0	0.0	0.0	8.424606928902214E-5	0.0010109528314682658
16	0.0	0.0	0.0	8.424606928902214E-5	0.001095198900757288
17	0.0	0.0	0.0	8.424606928902214E-5	0.001095198900757288
18	0.0	0.0	0.0	2.5273820786706646E-4	0.00117944497004631
19	0.0	0.0	0.0	3.369842771560886E-4	0.0013479371086243543
20	0.0	0.0	0.0	3.369842771560886E-4	0.0015164292472023987
21	0.0	0.0	0.0	5.054764157341329E-4	0.001684921385780443
22	0.0	0.0	0.0	6.739685543121772E-4	0.001684921385780443
23	0.0	0.0	0.0	0.00117944497004631	0.001684921385780443
24	0.0	0.0	0.0	0.0016006753164914207	0.001684921385780443
25	0.0	0.0	0.0	0.0016006753164914207	0.001684921385780443
26	0.0	0.0	0.0	0.002274643870803598	0.0018534135243584873
27	0.0	0.0	0.0	0.0032013506329828414	0.0018534135243584873
28	0.0	0.0	0.0	0.003875319187295019	0.0018534135243584873
29	0.0	0.0	0.0	0.005139010226630351	0.0018534135243584873
30	0.0	0.0	0.0	0.006739685543121772	0.0018534135243584873
31	0.0	0.0	0.0	0.0110362350768619	0.0019376595936475094
32	0.0	0.0	0.0	0.015501276749180075	0.0019376595936475094
33	0.0	0.0	0.0	0.019713580213631184	0.0019376595936475094
34	0.0	0.0	0.0	0.025021082578839577	0.0020219056629365317
35	0.0	0.0	0.0	0.03125529170622722	0.0020219056629365317
36	0.0	0.0	0.0	0.040185375050863566	0.0020219056629365317
37	0.0	0.0	0.0	0.053496253998529066	0.0020219056629365317
38	0.0	0.0	0.0	0.06908177681699816	0.0020219056629365317
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTA	40	7.03434E-4	27.498474	29
GTATCAA	1710	0.0	25.863659	1
GGTATCA	820	0.0	25.223425	1
ATCGATT	90	1.5133992E-8	24.443089	29
CGGATCG	90	4.0821942E-7	21.999704	26
ATACGAA	270	0.0	20.373531	40
GTCTAGC	130	1.6207196E-9	20.310843	1
TACGCAG	65	4.933938E-4	20.306564	5
ACCGTAT	55	0.0044850064	19.99889	8
CGAGCCG	290	0.0	19.723043	15
CGAATGC	270	0.0	19.559414	43
CGCAATA	275	0.0	19.200552	36
GTCTTAG	200	0.0	18.702902	1
GGGTACG	60	0.0074053966	18.336178	1
ATACCGT	325	0.0	18.275908	6
GCGCAAT	290	0.0	18.206654	35
TAGGACG	85	1.4314137E-4	18.116642	4
CAATACG	305	0.0	18.035585	38
AATACGA	305	0.0	18.035585	39
TACGAAT	320	0.0	17.877773	41
>>END_MODULE