FastQCFastQC Report
Thu 2 Feb 2017
SRR4062229_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062229_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1119
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGTGGTGCCCTTCCGTCAAT60.5361930294906166No Hit
CCCACGGAATCGAGAAAGAGCTATC40.3574620196604111No Hit
GGCTCCACTCCTGGTGGTGCCCTTC40.3574620196604111No Hit
GGACTCTTTCGAGGCCCTGTAATTG40.3574620196604111No Hit
GTACATGGGTACCTGGTTGATCCTG40.3574620196604111No Hit
GTCCAAATGTGCCCATCATCCTGGT40.3574620196604111No Hit
AGACGGACCAGAGCGAAAGCATTTG40.3574620196604111No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG40.3574620196604111No Hit
TTCGTATTGCGCCGCTAGAGGTGAA30.2680965147453083No Hit
GGTTGATCCTGCCAGTAGCATATGC30.2680965147453083No Hit
GGCGGTGACTCGCCTCGCGGCGGAC30.2680965147453083No Hit
GGGACGGGCGGTGACTCGCCTCGCG30.2680965147453083No Hit
GCATATGCTTGTCTCAAAGATTAAG30.2680965147453083No Hit
CTTCGGGCCCCGCGGGACACTCAGC20.17873100983020554No Hit
GTATCTGATCGTCTTCGAACCTCCG20.17873100983020554No Hit
CTCCAATGGATCCTCGTTAAAGGAT20.17873100983020554No Hit
CTACGACGGTATCTGATCGTCTTCG20.17873100983020554No Hit
TTCTTGGACCGGCGCAAGACGGACC20.17873100983020554No Hit
CTTAGCTGAGTGTCCCGCGGGGCCC20.17873100983020554No Hit
GTGCATGGCCGTTCTTAGTTGGTGG20.17873100983020554No Hit
GTACATGGGAATAACGCCGCCGCAT20.17873100983020554No Hit
GCGGTATCCAGGCGGCTCGGGCCTG20.17873100983020554No Hit
GAACTACGACGGTATCTGATCGTCT20.17873100983020554No Hit
CTCTTAGCTGAGTGTCCCGCGGGGC20.17873100983020554No Hit
CCATTATTCCTAGCTGCGGTATCCA20.17873100983020554No Hit
CATTCTTGGCAAATGCTTTCGCTCT20.17873100983020554No Hit
AAGCAGGCCCGAGCCGCCTGGATAC20.17873100983020554No Hit
GCTCCACTCCTGGTGGTGCCCTTCC20.17873100983020554No Hit
CACCACCACCCACGGAATCGAGAAA20.17873100983020554No Hit
ATCCTCGTTAAAGGATTTAAAGTGG20.17873100983020554No Hit
GTCAATCCTGTCCGTGTCCGGGCCG20.17873100983020554No Hit
TATCAACGCAGAGTACTTTTTTTTT20.17873100983020554No Hit
GTATCAACGCAGAGTACATGGGCAT20.17873100983020554No Hit
TCTTAGCTGAGTGTCCCGCGGGGCC20.17873100983020554No Hit
GTTCGAAGACGATCAGATACCGTCG20.17873100983020554No Hit
GAATTACCGCGGCTGCTGGCACCAG20.17873100983020554No Hit
AGCAAAGCCAGGAGGGTTTCGAGCA20.17873100983020554No Hit
ATATACATGTGGTGTGTCATCTCCA20.17873100983020554No Hit
ACCATACTCCCCCCGGAACCCAAAG20.17873100983020554No Hit
CTATTGGAGCTGGAATTACCGCGGC20.17873100983020554No Hit
TCCCCGCCCCTTGCCTCTCGGCGCC20.17873100983020554No Hit
GAATCGAGAAAGAGCTATCAATCTG20.17873100983020554No Hit
AAATTAGAGTGTTCAAAGCAGGCCC20.17873100983020554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG20.17873100983020554No Hit
GTTAAAAAGCTCGTAGTTGGATCTT20.17873100983020554No Hit
CTTTAATATACGCTATTGGAGCTGG20.17873100983020554No Hit
ACGCAGAGTACATGGGCTCTCTTCT20.17873100983020554No Hit
CCCCCCTTCCCGGGGGGGGATGCGT20.17873100983020554No Hit
CCAATGGATCCTCGTTAAAGGATTT20.17873100983020554No Hit
GGTTTCCCGTGTTGGGTCAAATTAA20.17873100983020554No Hit
GTATCAACGCAGAGTACTTTTTTTT20.17873100983020554No Hit
CTCCCGACCCGGGGAGGTAGTGACG20.17873100983020554No Hit
GTATCAACGCAGAGTACATGGGTAC20.17873100983020554No Hit
GCTCGGGCCTGCTTTGAACACTCTA20.17873100983020554No Hit
AAGGACAATAGTTCCATCGTGTGCT20.17873100983020554No Hit
GGGCTACACAGAGAAACCCTGTCTC20.17873100983020554No Hit
ATCTAGAGTCACCAAAGCCGCCGGC20.17873100983020554No Hit
CACCTCCTCCACTCCCAGCAGCTGT20.17873100983020554No Hit
TGCGTAAAGAGTCCCAGAAAGACCG20.17873100983020554No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)