##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062229_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1119
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.621983914209114	32.0	32.0	32.0	14.0	32.0
2	28.60053619302949	32.0	32.0	32.0	14.0	32.0
3	28.596961572832885	32.0	32.0	32.0	14.0	32.0
4	28.471849865951743	32.0	32.0	32.0	14.0	32.0
5	28.20822162645219	32.0	32.0	32.0	14.0	32.0
6	31.621983914209114	36.0	32.0	36.0	14.0	36.0
7	31.611260053619304	36.0	32.0	36.0	14.0	36.0
8	31.483467381590707	36.0	32.0	36.0	14.0	36.0
9	31.369079535299374	36.0	32.0	36.0	14.0	36.0
10	30.8704200178731	36.0	32.0	36.0	14.0	36.0
11	31.771224307417338	36.0	32.0	36.0	14.0	36.0
12	30.857908847184987	36.0	32.0	36.0	14.0	36.0
13	31.289544235924932	36.0	32.0	36.0	14.0	36.0
14	31.204647006255584	36.0	32.0	36.0	14.0	36.0
15	31.027703306523684	36.0	32.0	36.0	14.0	36.0
16	31.25201072386059	36.0	32.0	36.0	14.0	36.0
17	31.16443252904379	36.0	32.0	36.0	14.0	36.0
18	31.03395889186774	36.0	32.0	36.0	14.0	36.0
19	31.04289544235925	36.0	32.0	36.0	14.0	36.0
20	30.949955317247543	36.0	32.0	36.0	14.0	36.0
21	30.932082216264522	36.0	32.0	36.0	14.0	36.0
22	30.78909740840036	36.0	32.0	36.0	14.0	36.0
23	30.718498659517426	36.0	32.0	36.0	14.0	36.0
24	30.511170688114387	36.0	27.0	36.0	14.0	36.0
25	30.06970509383378	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	4.0
17	3.0
18	5.0
19	12.0
20	15.0
21	15.0
22	15.0
23	29.0
24	45.0
25	31.0
26	38.0
27	46.0
28	61.0
29	75.0
30	80.0
31	94.0
32	121.0
33	146.0
34	177.0
35	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.890974084003574	20.464700625558535	12.064343163538874	29.57998212689902
2	18.051831992850758	19.3923145665773	39.23145665773012	23.324396782841823
3	17.694369973190348	22.5201072386059	25.915996425379806	33.86952636282395
4	12.779267202859696	15.370866845397677	32.0822162645219	39.76764968722073
5	17.247542448614837	35.388739946380696	28.9544235924933	18.40929401251117
6	35.746201966041106	33.51206434316354	14.119749776586238	16.621983914209114
7	31.27792672028597	28.686327077747993	20.64343163538874	19.3923145665773
8	29.0437890974084	29.1331546023235	18.945487042001787	22.87756925826631
9	27.882037533512065	13.047363717605004	17.694369973190348	41.37622877569258
10	15.728328865058089	24.218051831992852	29.1331546023235	30.920464700625562
11	37.890974084003574	20.196604110813226	22.430741733690795	19.481680071492406
12	26.094727435210007	21.090259159964255	27.34584450402145	25.46916890080429
13	27.70330652368186	17.873100983020553	23.50312779267203	30.920464700625562
14	22.698838248436104	16.890080428954423	25.737265415549597	34.673815907059875
15	25.37980339588919	24.486148346738158	21.00089365504915	29.1331546023235
16	27.70330652368186	24.128686327077748	24.218051831992852	23.949955317247543
17	25.201072386058982	25.915996425379806	24.57551385165326	24.307417336907953
18	24.128686327077748	21.805183199285075	25.290437890974083	28.775692582663094
19	26.094727435210007	23.413762287756924	22.34137622877569	28.150134048257375
20	24.128686327077748	24.307417336907953	22.430741733690795	29.1331546023235
21	26.899016979445932	21.983914209115284	23.681858802502234	27.43521000893655
22	24.128686327077748	22.34137622877569	26.720285969615727	26.80965147453083
23	26.005361930294907	21.00089365504915	26.094727435210007	26.899016979445932
24	23.771224307417338	23.86058981233244	25.022341376228773	27.34584450402145
25	25.558534405719392	23.59249329758713	22.5201072386059	28.328865058087576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	2.0
13	2.0
14	2.0
15	2.0
16	2.0
17	2.0
18	3.5
19	5.0
20	5.0
21	5.0
22	10.0
23	15.0
24	15.0
25	15.0
26	19.0
27	23.0
28	23.0
29	23.0
30	33.5
31	44.0
32	44.0
33	44.0
34	52.0
35	60.0
36	60.0
37	60.0
38	71.0
39	82.0
40	82.0
41	82.0
42	84.5
43	87.0
44	87.0
45	87.0
46	106.5
47	126.0
48	126.0
49	126.0
50	148.0
51	170.0
52	170.0
53	170.0
54	147.5
55	125.0
56	125.0
57	125.0
58	109.5
59	94.0
60	94.0
61	94.0
62	95.5
63	97.0
64	97.0
65	97.0
66	84.5
67	72.0
68	72.0
69	72.0
70	61.0
71	50.0
72	50.0
73	50.0
74	36.0
75	22.0
76	22.0
77	22.0
78	24.0
79	26.0
80	26.0
81	26.0
82	16.5
83	7.0
84	7.0
85	7.0
86	5.0
87	3.0
88	3.0
89	3.0
90	3.0
91	3.0
92	3.0
93	3.0
94	2.0
95	1.0
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1119.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.67202859696158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.31051108968177	87.39946380697052
2	4.435872709739633	8.221626452189454
3	0.4821600771456124	1.3404825737265416
4	0.6750241080038573	2.5022341376228776
5	0.0	0.0
6	0.09643201542912247	0.5361930294906166
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCTGGTGGTGCCCTTCCGTCAAT	6	0.5361930294906166	No Hit
CCCACGGAATCGAGAAAGAGCTATC	4	0.3574620196604111	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	4	0.3574620196604111	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	4	0.3574620196604111	No Hit
GTACATGGGTACCTGGTTGATCCTG	4	0.3574620196604111	No Hit
GTCCAAATGTGCCCATCATCCTGGT	4	0.3574620196604111	No Hit
AGACGGACCAGAGCGAAAGCATTTG	4	0.3574620196604111	No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG	4	0.3574620196604111	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	3	0.2680965147453083	No Hit
GGTTGATCCTGCCAGTAGCATATGC	3	0.2680965147453083	No Hit
GGCGGTGACTCGCCTCGCGGCGGAC	3	0.2680965147453083	No Hit
GGGACGGGCGGTGACTCGCCTCGCG	3	0.2680965147453083	No Hit
GCATATGCTTGTCTCAAAGATTAAG	3	0.2680965147453083	No Hit
CTTCGGGCCCCGCGGGACACTCAGC	2	0.17873100983020554	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2	0.17873100983020554	No Hit
CTCCAATGGATCCTCGTTAAAGGAT	2	0.17873100983020554	No Hit
CTACGACGGTATCTGATCGTCTTCG	2	0.17873100983020554	No Hit
TTCTTGGACCGGCGCAAGACGGACC	2	0.17873100983020554	No Hit
CTTAGCTGAGTGTCCCGCGGGGCCC	2	0.17873100983020554	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2	0.17873100983020554	No Hit
GTACATGGGAATAACGCCGCCGCAT	2	0.17873100983020554	No Hit
GCGGTATCCAGGCGGCTCGGGCCTG	2	0.17873100983020554	No Hit
GAACTACGACGGTATCTGATCGTCT	2	0.17873100983020554	No Hit
CTCTTAGCTGAGTGTCCCGCGGGGC	2	0.17873100983020554	No Hit
CCATTATTCCTAGCTGCGGTATCCA	2	0.17873100983020554	No Hit
CATTCTTGGCAAATGCTTTCGCTCT	2	0.17873100983020554	No Hit
AAGCAGGCCCGAGCCGCCTGGATAC	2	0.17873100983020554	No Hit
GCTCCACTCCTGGTGGTGCCCTTCC	2	0.17873100983020554	No Hit
CACCACCACCCACGGAATCGAGAAA	2	0.17873100983020554	No Hit
ATCCTCGTTAAAGGATTTAAAGTGG	2	0.17873100983020554	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	2	0.17873100983020554	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2	0.17873100983020554	No Hit
GTATCAACGCAGAGTACATGGGCAT	2	0.17873100983020554	No Hit
TCTTAGCTGAGTGTCCCGCGGGGCC	2	0.17873100983020554	No Hit
GTTCGAAGACGATCAGATACCGTCG	2	0.17873100983020554	No Hit
GAATTACCGCGGCTGCTGGCACCAG	2	0.17873100983020554	No Hit
AGCAAAGCCAGGAGGGTTTCGAGCA	2	0.17873100983020554	No Hit
ATATACATGTGGTGTGTCATCTCCA	2	0.17873100983020554	No Hit
ACCATACTCCCCCCGGAACCCAAAG	2	0.17873100983020554	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2	0.17873100983020554	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCC	2	0.17873100983020554	No Hit
GAATCGAGAAAGAGCTATCAATCTG	2	0.17873100983020554	No Hit
AAATTAGAGTGTTCAAAGCAGGCCC	2	0.17873100983020554	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	2	0.17873100983020554	No Hit
GTTAAAAAGCTCGTAGTTGGATCTT	2	0.17873100983020554	No Hit
CTTTAATATACGCTATTGGAGCTGG	2	0.17873100983020554	No Hit
ACGCAGAGTACATGGGCTCTCTTCT	2	0.17873100983020554	No Hit
CCCCCCTTCCCGGGGGGGGATGCGT	2	0.17873100983020554	No Hit
CCAATGGATCCTCGTTAAAGGATTT	2	0.17873100983020554	No Hit
GGTTTCCCGTGTTGGGTCAAATTAA	2	0.17873100983020554	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2	0.17873100983020554	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	2	0.17873100983020554	No Hit
GTATCAACGCAGAGTACATGGGTAC	2	0.17873100983020554	No Hit
GCTCGGGCCTGCTTTGAACACTCTA	2	0.17873100983020554	No Hit
AAGGACAATAGTTCCATCGTGTGCT	2	0.17873100983020554	No Hit
GGGCTACACAGAGAAACCCTGTCTC	2	0.17873100983020554	No Hit
ATCTAGAGTCACCAAAGCCGCCGGC	2	0.17873100983020554	No Hit
CACCTCCTCCACTCCCAGCAGCTGT	2	0.17873100983020554	No Hit
TGCGTAAAGAGTCCCAGAAAGACCG	2	0.17873100983020554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE