##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062229_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1119
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.621090259159963	32.0	27.0	32.0	21.0	32.0
2	28.449508489722966	32.0	32.0	32.0	14.0	32.0
3	30.126899016979447	32.0	32.0	32.0	21.0	32.0
4	30.569258266309205	32.0	32.0	32.0	32.0	32.0
5	30.488829311885613	32.0	32.0	32.0	32.0	32.0
6	33.80071492403932	36.0	36.0	36.0	21.0	36.0
7	33.62913315460232	36.0	36.0	36.0	21.0	36.0
8	33.71492403932082	36.0	36.0	36.0	21.0	36.0
9	34.09294012511171	36.0	36.0	36.0	32.0	36.0
10	33.624664879356565	36.0	36.0	36.0	21.0	36.0
11	34.53172475424486	36.0	36.0	36.0	32.0	36.0
12	33.858802502234134	36.0	36.0	36.0	32.0	36.0
13	34.13047363717605	36.0	36.0	36.0	32.0	36.0
14	34.02055406613047	36.0	36.0	36.0	32.0	36.0
15	33.768543342269886	36.0	36.0	36.0	21.0	36.0
16	33.86327077747989	36.0	36.0	36.0	32.0	36.0
17	33.609472743521	36.0	36.0	36.0	21.0	36.0
18	34.14834673815907	36.0	36.0	36.0	32.0	36.0
19	33.786416443252904	36.0	36.0	36.0	32.0	36.0
20	33.8248436103664	36.0	36.0	36.0	32.0	36.0
21	33.66487935656836	36.0	36.0	36.0	21.0	36.0
22	33.744414655942805	36.0	36.0	36.0	27.0	36.0
23	33.510277033065236	36.0	36.0	36.0	21.0	36.0
24	33.535299374441465	36.0	36.0	36.0	21.0	36.0
25	33.72475424486149	36.0	36.0	36.0	32.0	36.0
26	33.68096514745308	36.0	36.0	36.0	27.0	36.0
27	33.58713136729222	36.0	36.0	36.0	21.0	36.0
28	33.44504021447721	36.0	36.0	36.0	21.0	36.0
29	33.19124218051832	36.0	36.0	36.0	21.0	36.0
30	32.90259159964254	36.0	36.0	36.0	14.0	36.0
31	33.35567470956211	36.0	36.0	36.0	21.0	36.0
32	33.28150134048257	36.0	36.0	36.0	21.0	36.0
33	33.403932082216265	36.0	36.0	36.0	21.0	36.0
34	33.24218051831993	36.0	36.0	36.0	21.0	36.0
35	33.1295799821269	36.0	36.0	36.0	21.0	36.0
36	33.00983020554066	36.0	36.0	36.0	14.0	36.0
37	33.22252010723861	36.0	36.0	36.0	21.0	36.0
38	33.17068811438784	36.0	36.0	36.0	21.0	36.0
39	32.67917783735478	36.0	32.0	36.0	14.0	36.0
40	32.597855227882036	36.0	32.0	36.0	14.0	36.0
41	33.11081322609473	36.0	36.0	36.0	21.0	36.0
42	32.864164432529044	36.0	36.0	36.0	14.0	36.0
43	32.50938337801609	36.0	36.0	36.0	14.0	36.0
44	32.43163538873995	36.0	32.0	36.0	14.0	36.0
45	32.79088471849866	36.0	36.0	36.0	14.0	36.0
46	32.56300268096515	36.0	32.0	36.0	14.0	36.0
47	32.58355674709562	36.0	36.0	36.0	14.0	36.0
48	32.727435210008935	36.0	36.0	36.0	14.0	36.0
49	32.39767649687221	36.0	32.0	36.0	14.0	36.0
50	32.080428954423596	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	0.0
24	6.0
25	5.0
26	11.0
27	19.0
28	21.0
29	45.0
30	77.0
31	119.0
32	137.0
33	224.0
34	272.0
35	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.41465594280608	16.80071492403932	13.85165326184093	24.932975871313673
2	23.50312779267203	17.158176943699733	30.205540661304735	29.1331546023235
3	26.899016979445932	18.67739052725648	25.022341376228773	29.40125111706881
4	18.230563002680967	14.119749776586238	33.51206434316354	34.13762287756926
5	24.57551385165326	26.094727435210007	30.831099195710454	18.498659517426276
6	40.303842716711344	33.33333333333333	12.243074173369079	14.119749776586238
7	30.920464700625562	29.2225201072386	16.44325290437891	23.413762287756924
8	31.009830205540663	27.882037533512065	16.264521894548704	24.84361036639857
9	26.362823949955317	11.081322609472743	16.890080428954423	45.66577301161752
10	17.694369973190348	21.447721179624665	26.184092940125115	34.673815907059875
11	44.05719392314567	15.281501340482572	17.694369973190348	22.966934763181413
12	26.630920464700626	20.91152815013405	24.664879356568363	27.79267202859696
13	28.150134048257375	18.766756032171582	21.00089365504915	32.0822162645219
14	26.094727435210007	14.655942806076855	21.26899016979446	37.980339588918675
15	28.775692582663094	24.396782841823057	19.749776586237715	27.07774798927614
16	28.060768543342267	23.50312779267203	19.034852546916888	29.40125111706881
17	25.826630920464698	28.239499553172475	20.285969615728327	25.647899910634496
18	27.16711349419124	22.87756925826631	23.86058981233244	26.094727435210007
19	29.49061662198391	22.966934763181413	21.805183199285075	25.737265415549597
20	29.49061662198391	20.10723860589812	21.89454870420018	28.507596067917785
21	28.865058087578195	22.34137622877569	22.698838248436104	26.094727435210007
22	29.57998212689902	22.25201072386059	22.073279714030384	26.094727435210007
23	26.630920464700626	21.35835567470956	23.413762287756924	28.596961572832885
24	26.630920464700626	22.430741733690795	22.87756925826631	28.060768543342267
25	26.184092940125115	23.86058981233244	23.949955317247543	26.005361930294907
26	27.61394101876676	24.57551385165326	21.35835567470956	26.452189454870418
27	27.882037533512065	21.715817694369974	24.128686327077748	26.273458445040216
28	25.11170688114388	23.324396782841823	22.966934763181413	28.596961572832885
29	25.915996425379806	22.78820375335121	24.664879356568363	26.630920464700626
30	25.37980339588919	22.698838248436104	22.966934763181413	28.9544235924933
31	29.57998212689902	25.11170688114388	21.179624664879356	24.128686327077748
32	29.31188561215371	23.949955317247543	21.537086684539766	25.201072386058982
33	25.46916890080429	23.056300268096514	21.447721179624665	30.02680965147453
34	27.43521000893655	22.78820375335121	23.324396782841823	26.452189454870418
35	27.16711349419124	23.771224307417338	22.966934763181413	26.094727435210007
36	26.720285969615727	22.78820375335121	24.307417336907953	26.184092940125115
37	27.882037533512065	22.87756925826631	22.78820375335121	26.452189454870418
38	27.43521000893655	21.35835567470956	24.218051831992852	26.988382484361033
39	25.915996425379806	25.201072386058982	20.554066130473636	28.328865058087576
40	27.256478999106342	24.664879356568363	22.073279714030384	26.005361930294907
41	25.737265415549597	22.25201072386059	24.664879356568363	27.34584450402145
42	27.882037533512065	23.50312779267203	22.966934763181413	25.647899910634496
43	25.290437890974083	26.005361930294907	26.273458445040216	22.430741733690795
44	26.452189454870418	26.005361930294907	21.35835567470956	26.184092940125115
45	26.005361930294907	24.664879356568363	22.609472743521	26.720285969615727
46	26.541554959785525	25.201072386058982	21.89454870420018	26.362823949955317
47	26.899016979445932	26.630920464700626	23.056300268096514	23.413762287756924
48	28.328865058087576	24.57551385165326	21.26899016979446	25.826630920464698
49	25.647899910634496	24.128686327077748	22.698838248436104	27.52457551385165
50	29.57998212689902	22.34137622877569	22.162645218945485	25.915996425379806
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	0.5
16	0.0
17	2.0
18	4.0
19	2.0
20	0.0
21	0.5
22	1.0
23	4.5
24	8.0
25	6.5
26	5.0
27	6.0
28	7.0
29	10.5
30	14.0
31	13.5
32	13.0
33	18.0
34	23.0
35	19.5
36	16.0
37	19.0
38	22.0
39	26.0
40	30.0
41	40.0
42	50.0
43	47.0
44	44.0
45	43.0
46	42.0
47	61.0
48	80.0
49	72.5
50	65.0
51	62.5
52	60.0
53	62.5
54	65.0
55	74.5
56	84.0
57	83.5
58	83.0
59	78.5
60	74.0
61	66.0
62	58.0
63	53.5
64	49.0
65	43.0
66	37.0
67	38.5
68	40.0
69	40.5
70	41.0
71	29.5
72	18.0
73	19.0
74	20.0
75	16.5
76	13.0
77	10.0
78	7.0
79	8.0
80	9.0
81	8.5
82	8.0
83	6.5
84	5.0
85	4.0
86	3.0
87	1.5
88	0.0
89	1.0
90	2.0
91	3.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	6.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1119.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.04647006255585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.95145631067962	87.39946380697052
2	3.3980582524271843	6.255585344057193
3	0.7766990291262136	2.1447721179624666
4	0.3883495145631068	1.4298480786416443
5	0.1941747572815534	0.8936550491510277
6	0.1941747572815534	1.0723860589812333
7	0.0	0.0
8	0.0	0.0
9	0.0970873786407767	0.8042895442359249
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	9	0.8042895442359249	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	6	0.5361930294906166	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	6	0.5361930294906166	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	5	0.44682752457551383	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC	5	0.44682752457551383	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG	4	0.3574620196604111	No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	4	0.3574620196604111	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG	4	0.3574620196604111	No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC	4	0.3574620196604111	No Hit
GTACATGGAGCGCCATTTCGTGACGCCGCGAGACCGAGAATCTGTAGGAG	3	0.2680965147453083	No Hit
CCTCTGACACTGGCAGGCCGAGCCCTCGTAACCGGGCTTGCAACTACATT	3	0.2680965147453083	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3	0.2680965147453083	No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG	3	0.2680965147453083	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC	3	0.2680965147453083	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	3	0.2680965147453083	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3	0.2680965147453083	No Hit
CCCTGACGCACCGCCGTGATGCCCAGGGAAGACAGGGCGACCTGGAAGTC	3	0.2680965147453083	No Hit
TTTTCTAGCTGTGTAGCTGAAAATGCCATTGACTTCTGACCTGCCTCCGC	2	0.17873100983020554	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2	0.17873100983020554	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG	2	0.17873100983020554	No Hit
TCCACGGCCTCCTCGACCCCTTCCAAGACCACCTCGGCCTCGGATAGGCC	2	0.17873100983020554	No Hit
GGCATGGCCTGCCTCCTCACCCGAGTACAGGGTCCATCCACTAAAGCCCT	2	0.17873100983020554	No Hit
ATGTAGCACCAGGGCCCGCGCGGGTCCTGGTCCGGGTTCCGGCAGTAGTT	2	0.17873100983020554	No Hit
TGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAG	2	0.17873100983020554	No Hit
CTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGT	2	0.17873100983020554	No Hit
CCTCGAGACCGTAAGAAGCCCGCACCCTTTCCGGAAGATAGCTAGAGAAG	2	0.17873100983020554	No Hit
GAGGAAAGCCATTCGCCCTGGGCCTGGACTAGACCTCGTCGGGCCGGCGG	2	0.17873100983020554	No Hit
GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT	2	0.17873100983020554	No Hit
CGTCTAGCTTGCAGAGCAGCTCTCGTGGCCATCTCAAAAACCTCTCTCAC	2	0.17873100983020554	No Hit
GGGCATGGCCTGCCTCCTCACCCGAGTACAGGGTCCATCCACTAAAGCCC	2	0.17873100983020554	No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC	2	0.17873100983020554	No Hit
GGGTCGGTCCGGCCGCATGCACTCTCCCGTTCCGCGCGAGCGCCCGCCCG	2	0.17873100983020554	No Hit
TCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAG	2	0.17873100983020554	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG	2	0.17873100983020554	No Hit
ATGTGGAACTGCAGATCCGCTCCTACGACCGCCAGACCGGTCGCCCCGGC	2	0.17873100983020554	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG	2	0.17873100983020554	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	2	0.17873100983020554	No Hit
CTCAAGTACTCTGCCGACGAGGCTCGTTCACTCAAAGCATACGGCGAATT	2	0.17873100983020554	No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGCCGACCCATTCGAACG	2	0.17873100983020554	No Hit
GGGCCTAAGCCGCGCCAGGCGAGGGACGGACATTCATGGCGAATGGCGGC	2	0.17873100983020554	No Hit
GTCTGTGTTCAGACACACCAGAAGTGGACATCAGATCCCATTACAGATGG	2	0.17873100983020554	No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT	2	0.17873100983020554	No Hit
CTCCGCCGCCGCCTCCTCCTCCTCTCTCGCGCTCTCTGTCCCGCCTGGTC	2	0.17873100983020554	No Hit
GAGTACATGGAGCGCCATTTCGTGACGCCGCGAGACCGAGAATCTGTAGG	2	0.17873100983020554	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	2	0.17873100983020554	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	2	0.17873100983020554	No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCA	2	0.17873100983020554	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2	0.17873100983020554	No Hit
CAGAAGGACCCTGGCTGGTCAGACAGGTTGGACATTATTAATAATCGTGG	2	0.17873100983020554	No Hit
TCCCTGACGCACCGCCGTGATGCCCAGGGAAGACAGGGCGACCTGGAAGT	2	0.17873100983020554	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC	2	0.17873100983020554	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	2	0.17873100983020554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE