Basic Statistics
Measure | Value |
---|---|
Filename | SRR4062228_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 522713 |
Sequences flagged as poor quality | 0 |
Sequence length | 25 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATCAACGCAGAGTACTTTTTTTT | 1546 | 0.29576459739857247 | No Hit |
TATCAACGCAGAGTACTTTTTTTTT | 1036 | 0.19819671597989721 | No Hit |
GGTATCAACGCAGAGTACTTTTTTT | 603 | 0.1153596715597278 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTT | 560 | 0.1071333599891336 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCAAT | 25 | 0.006027883 | 18.999424 | 19 |
CCGTCGT | 50 | 8.691419E-5 | 15.200996 | 9 |
GCACCGC | 40 | 0.0052672443 | 14.2523 | 6 |
TAACGCC | 40 | 0.0052705947 | 14.250935 | 4 |
CGCCGCA | 40 | 0.0052705947 | 14.250935 | 10 |
TAGGGTC | 55 | 1.9551412E-4 | 13.819088 | 4 |
ACCGTCG | 55 | 1.9551412E-4 | 13.819088 | 8 |
ATACCGT | 70 | 7.2283983E-6 | 13.573619 | 6 |
ACGCCGC | 50 | 0.0014954014 | 13.302146 | 6 |
GGGTTCC | 50 | 0.0014954014 | 13.302146 | 7 |
TTAGGAC | 100 | 1.0029908E-8 | 13.298324 | 3 |
TACCGTC | 60 | 4.0783818E-4 | 12.668711 | 7 |
CGTTCTT | 60 | 4.0816807E-4 | 12.667497 | 10 |
CGTCGTA | 60 | 4.0816807E-4 | 12.667497 | 10 |
GTATTAA | 60 | 4.0948996E-4 | 12.662643 | 1 |
GTTTAGG | 60 | 4.0948996E-4 | 12.662643 | 1 |
GTTTAGA | 60 | 4.0948996E-4 | 12.662643 | 1 |
CCGTTCT | 70 | 1.0875594E-4 | 12.215086 | 9 |
ATGGCCG | 55 | 0.003060052 | 12.09286 | 5 |
TGGCCGT | 55 | 0.003060052 | 12.09286 | 6 |