FastQCFastQC Report
Thu 2 Feb 2017
SRR4062228_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062228_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522713
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15460.29576459739857247No Hit
TATCAACGCAGAGTACTTTTTTTTT10360.19819671597989721No Hit
GGTATCAACGCAGAGTACTTTTTTT6030.1153596715597278No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5600.1071333599891336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAT250.00602788318.99942419
CCGTCGT508.691419E-515.2009969
GCACCGC400.005267244314.25236
TAACGCC400.005270594714.2509354
CGCCGCA400.005270594714.25093510
TAGGGTC551.9551412E-413.8190884
ACCGTCG551.9551412E-413.8190888
ATACCGT707.2283983E-613.5736196
ACGCCGC500.001495401413.3021466
GGGTTCC500.001495401413.3021467
TTAGGAC1001.0029908E-813.2983243
TACCGTC604.0783818E-412.6687117
CGTTCTT604.0816807E-412.66749710
CGTCGTA604.0816807E-412.66749710
GTATTAA604.0948996E-412.6626431
GTTTAGG604.0948996E-412.6626431
GTTTAGA604.0948996E-412.6626431
CCGTTCT701.0875594E-412.2150869
ATGGCCG550.00306005212.092865
TGGCCGT550.00306005212.092866