##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062228_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522713
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268558463248475	32.0	32.0	32.0	32.0	32.0
2	30.916745900714158	32.0	32.0	32.0	32.0	32.0
3	30.95162163558205	32.0	32.0	32.0	32.0	32.0
4	30.94003592793751	32.0	32.0	32.0	32.0	32.0
5	30.932226671232588	32.0	32.0	32.0	32.0	32.0
6	34.5677781115067	36.0	36.0	36.0	32.0	36.0
7	34.48994572547459	36.0	36.0	36.0	32.0	36.0
8	34.44474118684632	36.0	36.0	36.0	32.0	36.0
9	34.536064723854196	36.0	36.0	36.0	32.0	36.0
10	34.299870100801016	36.0	36.0	36.0	32.0	36.0
11	34.54572203101893	36.0	36.0	36.0	32.0	36.0
12	34.40581351525598	36.0	36.0	36.0	32.0	36.0
13	34.45188277314703	36.0	36.0	36.0	32.0	36.0
14	34.38631715683368	36.0	36.0	36.0	32.0	36.0
15	34.36784047842698	36.0	36.0	36.0	32.0	36.0
16	34.360666369499135	36.0	36.0	36.0	32.0	36.0
17	34.311260672682714	36.0	36.0	36.0	32.0	36.0
18	34.32131016446884	36.0	36.0	36.0	32.0	36.0
19	34.30052437953523	36.0	36.0	36.0	32.0	36.0
20	34.26307361783618	36.0	36.0	36.0	32.0	36.0
21	34.24344908200102	36.0	36.0	36.0	32.0	36.0
22	34.236634635067425	36.0	36.0	36.0	32.0	36.0
23	34.22096829426473	36.0	36.0	36.0	32.0	36.0
24	34.18720406800673	36.0	36.0	36.0	32.0	36.0
25	33.85152846782077	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	6.0
5	40.0
6	110.0
7	28.0
8	82.0
9	75.0
10	39.0
11	6.0
12	25.0
13	25.0
14	95.0
15	137.0
16	225.0
17	291.0
18	366.0
19	486.0
20	656.0
21	978.0
22	1391.0
23	1992.0
24	2700.0
25	3775.0
26	5187.0
27	6566.0
28	8854.0
29	11999.0
30	15755.0
31	21978.0
32	31811.0
33	46183.0
34	105962.0
35	254889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11819286213065	18.77751312882027	12.124047024612352	24.980246984436725
2	15.546993373023769	20.260678920705857	39.94015825158022	24.252169454690154
3	19.07711733083354	24.41985039505653	28.967496317269614	27.535535956840313
4	11.873830506139724	16.603847228227803	38.125978165621376	33.3963441000111
5	13.275066215533476	37.9656138524427	34.91740282009278	13.841917111931043
6	31.999777932204232	37.484086736618025	17.664727402208808	12.851407928968936
7	28.735416459196728	31.5854788916892	21.06005009920255	18.619054549911525
8	25.940381687429976	35.434305905334625	19.8749413488332	18.750371058402198
9	26.12716143020218	15.6142024255518	19.95219379898412	38.3064423452619
10	14.935443086471784	28.418834527412745	33.33531282326347	23.310409562851998
11	34.96660778806769	22.892535700738346	23.60999888884206	18.530857622351903
12	23.447625870890747	25.327811226708402	30.272229384529037	20.952333517871818
13	28.256488842064936	21.443539890815057	26.191169428215687	24.10880183890432
14	21.585324277839703	21.588197213635336	27.361840517588114	29.464637990936843
15	23.651977686849644	29.48925719890273	23.515777052396057	23.342988061851564
16	23.24093244897138	28.140101013389923	25.989209149813547	22.629757387825148
17	21.7820777051617	28.021782269248156	26.941671327546178	23.25446869804396
18	22.04991189764805	27.64441124645675	28.21975791005899	22.085918945836205
19	23.525063719698437	26.923894456051123	27.409523773048722	22.14151805120172
20	23.670131282648324	26.98340967232954	27.222958378651636	22.123500666370504
21	24.133170183390494	26.294523944934976	26.77286732194731	22.79943854972722
22	23.316723034437324	27.121451798853236	27.109769690098553	22.452055476610884
23	22.721224154535726	27.125507623203333	27.2892107213084	22.86405750095254
24	22.801474460242233	26.960984250083776	27.74034180669252	22.497199482981472
25	22.81942678574164	27.16085078353579	27.605467134388263	22.41425529633431
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	374.0
1	374.0
2	345.0
3	316.0
4	316.0
5	316.0
6	664.0
7	1012.0
8	1012.0
9	1012.0
10	1232.5
11	1453.0
12	1453.0
13	1453.0
14	1904.0
15	2355.0
16	2355.0
17	2355.0
18	4140.0
19	5925.0
20	5925.0
21	5925.0
22	9072.0
23	12219.0
24	12219.0
25	12219.0
26	17901.0
27	23583.0
28	23583.0
29	23583.0
30	29775.5
31	35968.0
32	35968.0
33	35968.0
34	40952.5
35	45937.0
36	45937.0
37	45937.0
38	50060.5
39	54184.0
40	54184.0
41	54184.0
42	56727.5
43	59271.0
44	59271.0
45	59271.0
46	63345.5
47	67420.0
48	67420.0
49	67420.0
50	65912.0
51	64404.0
52	64404.0
53	64404.0
54	58071.5
55	51739.0
56	51739.0
57	51739.0
58	45543.0
59	39347.0
60	39347.0
61	39347.0
62	32871.5
63	26396.0
64	26396.0
65	26396.0
66	20902.0
67	15408.0
68	15408.0
69	15408.0
70	11593.0
71	7778.0
72	7778.0
73	7778.0
74	5866.0
75	3954.0
76	3954.0
77	3954.0
78	3009.0
79	2064.0
80	2064.0
81	2064.0
82	1450.0
83	836.0
84	836.0
85	836.0
86	602.5
87	369.0
88	369.0
89	369.0
90	254.0
91	139.0
92	139.0
93	139.0
94	86.5
95	34.0
96	34.0
97	34.0
98	131.0
99	228.0
100	228.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0015304765712733375
2	1.9130957140916718E-4
3	5.739287142275015E-4
4	0.009756788141867525
5	0.03386179413942259
6	0.0669583499932085
7	0.10502895470363277
8	0.10675074084631528
9	0.11574229070254614
10	0.13257753298655284
11	0.1406125349857379
12	0.13200360427232535
13	0.12683824584427783
14	0.11459443327409113
15	0.13181229470091618
16	0.11555098113113697
17	0.12186419698763948
18	0.11344657584563614
19	0.09642002399022025
20	0.0920199038478094
21	0.09450692827612858
22	0.10464633556081443
23	0.08149787742030522
24	0.09335907084767359
25	0.08895895070526273
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	522713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.39165269617094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57855523706755	47.01284067088593
2	14.672039106894697	18.014814583902343
3	4.371625561036996	8.051439544826602
4	1.6766144535685399	4.117205289554408
5	0.8235651814141219	2.5280013795017395
6	0.43669379602804986	1.608561231619593
7	0.2761194741898438	1.1866001603478558
8	0.16691104837110216	0.8197556090201995
9	0.12496387607293978	0.6904564991493539
>10	0.7669863124277719	8.930187576015863
>50	0.07301996358759036	3.0356168853666614
>100	0.03165242784207095	3.283999308450015
>500	6.267807493479255E-4	0.22375600185876812
>1k	6.267807493479255E-4	0.49676525950072165
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1546	0.29576459739857247	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1036	0.19819671597989721	No Hit
GGTATCAACGCAGAGTACTTTTTTT	603	0.1153596715597278	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	560	0.1071333599891336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.9130957140916718E-4	0.0
12	0.0	0.0	0.0	1.9130957140916718E-4	0.0
13	0.0	0.0	0.0	1.9130957140916718E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCAAT	25	0.006027883	18.999424	19
CCGTCGT	50	8.691419E-5	15.200996	9
GCACCGC	40	0.0052672443	14.2523	6
TAACGCC	40	0.0052705947	14.250935	4
CGCCGCA	40	0.0052705947	14.250935	10
TAGGGTC	55	1.9551412E-4	13.819088	4
ACCGTCG	55	1.9551412E-4	13.819088	8
ATACCGT	70	7.2283983E-6	13.573619	6
ACGCCGC	50	0.0014954014	13.302146	6
GGGTTCC	50	0.0014954014	13.302146	7
TTAGGAC	100	1.0029908E-8	13.298324	3
TACCGTC	60	4.0783818E-4	12.668711	7
CGTTCTT	60	4.0816807E-4	12.667497	10
CGTCGTA	60	4.0816807E-4	12.667497	10
GTATTAA	60	4.0948996E-4	12.662643	1
GTTTAGG	60	4.0948996E-4	12.662643	1
GTTTAGA	60	4.0948996E-4	12.662643	1
CCGTTCT	70	1.0875594E-4	12.215086	9
ATGGCCG	55	0.003060052	12.09286	5
TGGCCGT	55	0.003060052	12.09286	6
>>END_MODULE