FastQCFastQC Report
Thu 2 Feb 2017
SRR4062228_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062228_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522713
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.11995110127354781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG250.002352377635.197555
TGCGTAG300.005744021429.33129313
GCGGCGT609.715521E-625.67470440
ATACCGT801.307726E-724.7482786
CGTCGTA551.5926825E-423.99833310
CCTATAC500.002579538221.998473
GAGCCGC755.4679484E-520.5319066
TACCGTC755.4679484E-520.5319067
GTATCAA10450.020.0043491
TAATCGT550.0044746320.00434938
AACCGTC550.0044820719.9986134
TACACGG550.0044820719.998615
TAGACAG901.00077905E-519.5541935
GGTATCA5650.019.473261
CGGCGTT808.954579E-519.25602741
CGCAATA808.974343E-519.25050236
CGCCGGT808.980939E-519.248667
GTATTAG1152.0113112E-719.1345941
GCATAGA708.103043E-418.8612441
CGACCAT708.118698E-418.85583121
Produced by FastQC (version 0.11.5)