##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062228_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522713
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238364073592965	32.0	32.0	32.0	32.0	32.0
2	31.456962042267936	32.0	32.0	32.0	32.0	32.0
3	31.585166238452075	32.0	32.0	32.0	32.0	32.0
4	31.664934677346842	32.0	32.0	32.0	32.0	32.0
5	31.652587557608094	32.0	32.0	32.0	32.0	32.0
6	35.28424010881688	36.0	36.0	36.0	36.0	36.0
7	35.28702366308089	36.0	36.0	36.0	36.0	36.0
8	35.24092188256271	36.0	36.0	36.0	36.0	36.0
9	35.310943098794176	36.0	36.0	36.0	36.0	36.0
10	35.21372148769975	36.0	36.0	36.0	36.0	36.0
11	35.33410494860468	36.0	36.0	36.0	36.0	36.0
12	35.255176358728406	36.0	36.0	36.0	36.0	36.0
13	35.28409662663833	36.0	36.0	36.0	36.0	36.0
14	35.25330917731145	36.0	36.0	36.0	36.0	36.0
15	35.23470432149191	36.0	36.0	36.0	36.0	36.0
16	35.24746849609633	36.0	36.0	36.0	36.0	36.0
17	35.231329620652254	36.0	36.0	36.0	36.0	36.0
18	35.22852310923968	36.0	36.0	36.0	36.0	36.0
19	35.21852144484641	36.0	36.0	36.0	36.0	36.0
20	35.203788694752184	36.0	36.0	36.0	36.0	36.0
21	35.187328419228145	36.0	36.0	36.0	36.0	36.0
22	35.175756103253605	36.0	36.0	36.0	36.0	36.0
23	35.15057785056044	36.0	36.0	36.0	36.0	36.0
24	35.14290442365122	36.0	36.0	36.0	36.0	36.0
25	35.13326816053934	36.0	36.0	36.0	36.0	36.0
26	35.09797537080578	36.0	36.0	36.0	36.0	36.0
27	35.07630382255655	36.0	36.0	36.0	36.0	36.0
28	35.0580509763484	36.0	36.0	36.0	36.0	36.0
29	35.03905010971604	36.0	36.0	36.0	36.0	36.0
30	35.00810196034918	36.0	36.0	36.0	36.0	36.0
31	35.00558815258086	36.0	36.0	36.0	36.0	36.0
32	34.98031998438914	36.0	36.0	36.0	36.0	36.0
33	34.96039509252687	36.0	36.0	36.0	36.0	36.0
34	34.9421250284573	36.0	36.0	36.0	36.0	36.0
35	34.936108342436484	36.0	36.0	36.0	36.0	36.0
36	34.89937116543878	36.0	36.0	36.0	36.0	36.0
37	34.8869379563929	36.0	36.0	36.0	36.0	36.0
38	34.86957661278751	36.0	36.0	36.0	36.0	36.0
39	34.820895979246735	36.0	36.0	36.0	32.0	36.0
40	34.82692031765041	36.0	36.0	36.0	32.0	36.0
41	34.796584358912064	36.0	36.0	36.0	32.0	36.0
42	34.7655137714195	36.0	36.0	36.0	32.0	36.0
43	34.75733720033747	36.0	36.0	36.0	32.0	36.0
44	34.69692355078217	36.0	36.0	36.0	32.0	36.0
45	34.65811831731753	36.0	36.0	36.0	32.0	36.0
46	34.61032727328381	36.0	36.0	36.0	32.0	36.0
47	34.56909814754942	36.0	36.0	36.0	32.0	36.0
48	34.55814758768196	36.0	36.0	36.0	32.0	36.0
49	34.51252982037944	36.0	36.0	36.0	32.0	36.0
50	34.118659761666535	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	14.0
22	32.0
23	127.0
24	262.0
25	551.0
26	1275.0
27	2380.0
28	4244.0
29	6566.0
30	9801.0
31	14575.0
32	21753.0
33	35974.0
34	80640.0
35	344508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66748577093405	19.358802117422808	12.595259146983285	25.378452964659857
2	14.938873902313036	20.922152710019322	39.7095792917408	24.429394095926842
3	18.40286811858155	24.73752276588255	29.412372396271753	27.447236719264147
4	11.672010590918134	16.970147997367572	38.163654172852354	33.194187238861936
5	12.858681532695762	38.42567527495968	34.72326113947807	13.992382052866487
6	32.01629195426927	37.756164006183134	17.583870276557644	12.643673762989946
7	28.73259322037141	31.539678561658118	21.08250607886163	18.645222139108842
8	25.90695085065801	35.67885244866686	19.77050503813756	18.643691662537567
9	26.093668992353358	15.336331792015887	19.933692102549582	38.63630711308117
10	14.907444252284241	28.415647622400098	33.2483279511471	23.428580174168566
11	35.14088993386429	22.786691740974494	23.585600511179177	18.48681781398205
12	23.42623963819534	25.223975680727285	30.338254453208545	21.01153022786883
13	28.186213084426825	21.474690700250424	26.32955369390086	24.00954252142189
14	21.706749210369743	21.444272478396368	27.46382814278581	29.385150168448078
15	23.699429706167628	29.544893660574733	23.45531869304953	23.30035794020811
16	23.373145007470665	27.865302241582825	26.12476110125863	22.636791649687876
17	21.68876611065728	27.902883609169848	27.133436512962177	23.274913767210688
18	21.99007868562672	27.488507077497594	28.571319251673483	21.950094985202202
19	23.641271596459244	26.60331769058738	27.717504634474366	22.037906078479015
20	23.750026305166717	26.789373095266793	27.54390093187249	21.916699667694004
21	24.129493622695435	26.095390778496043	27.157732828531145	22.61738277027738
22	23.333071876919075	26.838245844277836	27.376017049509006	22.452665229294087
23	22.739673089578964	26.78166944703776	27.676043404398598	22.80261405898468
24	22.7359165572999	26.873806690567932	27.945537261864224	22.44473949026795
25	22.911469413367207	26.682762209400206	27.996487549549276	22.409280827683315
26	22.067069566165625	27.30744139687131	28.38145744015336	22.24403159680971
27	22.414680387441628	27.29634539927417	27.74420281953125	22.544771393752953
28	22.251662977538086	27.08056329836792	27.73312773695397	22.934645987140023
29	22.46862989300015	26.880054179077923	27.55423168825604	23.097084239665897
30	22.749264884533783	27.09108544557467	27.711700819005674	22.44794885088587
31	23.14399954085439	26.51336305025731	27.576285129421667	22.766352279466627
32	22.410705745059403	27.334659753974478	27.55868454783723	22.695949953128885
33	22.602651933278874	27.014633269117088	27.811820253179086	22.570894544424952
34	22.74243844579212	27.09590403856823	28.05590097759752	22.105756538042126
35	22.948922257529468	27.26390964066323	27.69148653276272	22.095681569044583
36	22.25944256217083	27.213021294668394	27.86921312460184	22.65832301855894
37	23.043399240489375	26.983671478176024	27.11414660276829	22.858782678566303
38	22.516031772754406	27.23813495768224	27.694026538514514	22.55180673104884
39	22.8997995071856	27.16352408209492	27.26759668804236	22.669079722677115
40	23.436761664622843	27.083122095681567	27.53595185120707	21.94416438848852
41	22.780109660114864	26.9482271104598	28.15349355086798	22.118169678557354
42	22.95811483266404	27.71714071985169	27.143187849035087	22.181556598449177
43	22.54459118450027	27.268013920217033	27.82245027195696	22.364944623325737
44	22.37521020702163	27.435483901824952	27.41290876776118	22.77639712339224
45	22.493547936583248	27.748942512067128	27.327860478552747	22.429649072796877
46	22.748908555926704	27.013097328879553	27.056334201897066	23.181659913296677
47	22.91806164029768	27.289893057335807	27.138948939182338	22.653096363184176
48	22.69296626139527	27.554883823262134	26.829221630204998	22.922928285137605
49	22.944961096454392	27.310155097636706	26.900170842715653	22.84471296319325
50	22.446355872539716	27.717407940249167	27.047812911322662	22.788423275888455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	202.0
1	114.5
2	27.0
3	99.5
4	172.0
5	172.0
6	172.0
7	130.5
8	89.0
9	139.5
10	190.0
11	330.0
12	470.0
13	824.5
14	1179.0
15	1578.0
16	1977.0
17	2141.5
18	2306.0
19	2388.0
20	2470.0
21	2780.5
22	3091.0
23	3452.0
24	3813.0
25	4936.0
26	6059.0
27	7828.0
28	9597.0
29	11485.0
30	13373.0
31	15129.0
32	16885.0
33	19140.5
34	21396.0
35	23840.0
36	26284.0
37	28412.5
38	30541.0
39	31694.0
40	32847.0
41	33849.0
42	34851.0
43	34237.5
44	33624.0
45	34080.5
46	34537.0
47	35003.0
48	35469.0
49	35412.5
50	35356.0
51	33970.0
52	32584.0
53	31167.0
54	29750.0
55	28758.5
56	27767.0
57	25806.5
58	23846.0
59	21127.0
60	18408.0
61	16087.5
62	13767.0
63	11588.0
64	9409.0
65	7821.5
66	6234.0
67	5437.0
68	4640.0
69	4048.5
70	3457.0
71	2821.0
72	2185.0
73	1888.5
74	1592.0
75	1196.5
76	801.0
77	666.0
78	531.0
79	416.0
80	301.0
81	250.0
82	199.0
83	169.5
84	140.0
85	106.5
86	73.0
87	52.0
88	31.0
89	21.0
90	11.0
91	8.0
92	5.0
93	2.5
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036540128139150925
2	0.004400120142410845
3	1.9130957140916718E-4
4	1.9130957140916718E-4
5	0.0
6	1.9130957140916718E-4
7	0.0
8	0.0
9	0.0
10	1.9130957140916718E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.8261914281833437E-4
17	0.0
18	0.0
19	0.0
20	3.8261914281833437E-4
21	0.0
22	0.0
23	1.9130957140916718E-4
24	0.0013391669998641701
25	1.9130957140916718E-4
26	3.8261914281833437E-4
27	3.8261914281833437E-4
28	0.001147857428455003
29	3.8261914281833437E-4
30	0.001147857428455003
31	5.739287142275015E-4
32	5.739287142275015E-4
33	0.0
34	5.739287142275015E-4
35	0.0
36	0.0
37	0.0015304765712733375
38	1.9130957140916718E-4
39	1.9130957140916718E-4
40	0.0
41	0.0013391669998641701
42	0.004208810571001678
43	0.0038261914281833434
44	0.0026783339997283402
45	0.002295714856910006
46	0.002104405285500839
47	5.739287142275015E-4
48	0.003443572285365009
49	0.0019130957140916717
50	0.0017217861426825046
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	522713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.70453455887601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.76738272535802	48.904303858220324
2	14.55576617462309	18.545366186043903
3	4.4527179827484265	8.509749798387775
4	1.717915193072732	4.377559511452802
5	0.7875355913202471	2.5084794146802767
6	0.4292039089780361	1.6405341151377588
7	0.2490074574521437	1.1104032925074554
8	0.16858111500548306	0.8591505173472519
9	0.11436497176354776	0.6557010566432226
>10	0.6777577030730162	8.116348061322277
>50	0.05740819757727851	2.547694807906051
>100	0.02205683066273045	2.104023034834377
>500	3.0214836524285676E-4	0.12068634551651955
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.11995110127354781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	3.8261914281833437E-4	0.0
25	0.0	0.0	0.0	0.001147857428455003	0.0
26	0.0	0.0	0.0	0.0017217861426825046	0.0
27	0.0	0.0	0.0	0.002104405285500839	0.0
28	0.0	0.0	0.0	0.003060953142546675	0.0
29	0.0	0.0	0.0	0.005739287142275016	0.0
30	0.0	0.0	0.0	0.00880024028482169	0.0
31	0.0	0.0	0.0	0.018365718855280048	0.0
32	0.0	0.0	0.0	0.03041822185405758	0.0
33	0.0	0.0	0.0	0.03826191428183343	0.0
34	0.0	0.0	0.0	0.04916655985215596	0.0
35	0.0	0.0	0.0	0.06160168199375183	0.0
36	0.0	0.0	0.0	0.07767168599212187	0.0
37	0.0	0.0	0.0	0.10292454941813194	0.0
38	0.0	0.0	0.0	0.13181229470091618	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	25	0.0023523776	35.19755	5
TGCGTAG	30	0.0057440214	29.331293	13
GCGGCGT	60	9.715521E-6	25.674704	40
ATACCGT	80	1.307726E-7	24.748278	6
CGTCGTA	55	1.5926825E-4	23.998333	10
CCTATAC	50	0.0025795382	21.99847	3
GAGCCGC	75	5.4679484E-5	20.531906	6
TACCGTC	75	5.4679484E-5	20.531906	7
GTATCAA	1045	0.0	20.004349	1
TAATCGT	55	0.00447463	20.004349	38
AACCGTC	55	0.00448207	19.99861	34
TACACGG	55	0.00448207	19.99861	5
TAGACAG	90	1.00077905E-5	19.554193	5
GGTATCA	565	0.0	19.47326	1
CGGCGTT	80	8.954579E-5	19.256027	41
CGCAATA	80	8.974343E-5	19.250502	36
CGCCGGT	80	8.980939E-5	19.24866	7
GTATTAG	115	2.0113112E-7	19.134594	1
GCATAGA	70	8.103043E-4	18.861244	1
CGACCAT	70	8.118698E-4	18.855831	21
>>END_MODULE