FastQCFastQC Report
Thu 2 Feb 2017
SRR4062227_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062227_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179450
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA10010.5578155475062692No Hit
GATATACACTGTTCTACAAATCCCG8960.49930342713847864No Hit
GTATCAACGCAGAGTACTTTTTTTT8630.48091390359431596No Hit
GTCCTACAGTGTGCATTTCTCATTT7450.4151574254667038No Hit
GTGTATATCAATGAGTTACAATGAG6630.3694622457509056No Hit
TTGTAGAACAGTGTATATCAATGAG6560.36556143772638616No Hit
GTCCTACAGTGGACATTTCTAAATT6470.3605461131234327No Hit
TATCAACGCAGAGTACTTTTTTTTT6320.35218723878517694No Hit
CTGTAGGACCTGGAATATGGCGAGA5110.2847589857899136No Hit
CTGTAGGACGTGGAATATGGCAAGA5070.2825299526330454No Hit
GTCCTAAAGTGTGTATTTCTCATTT4940.27528559487322374No Hit
GTTCTACAAATCCCGTTTCCAACGA4440.24742268041237112No Hit
CTTTAGGACGTGAAATATGGCGAGG4410.24575090554471998No Hit
GTGTATATCAATGAGTTACAATGAA4390.24463638896628587No Hit
GTTCTACAGTGTGGTTTTTATCATT4270.23794928949568125No Hit
GGTATCAACGCAGAGTACTTTTTTT4030.22457509055447197No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3750.20897185845639454No Hit
ATGTAGGCAAGGGAAGTCGGCAAAA3700.2061855670103093No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3350.18668152688771245No Hit
GTACATGGGGTGGTATCAACGCAAA3110.1733073279465032No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2850.15881861242685985No Hit
GTATCAACGCAGAGTACATGGGGTG2670.14878796322095292No Hit
CTGTAGGACATGGAATATGGCAAGA2590.14432989690721648No Hit
GATATACACTGTTCTACAATGCCGG2560.14265812203956535No Hit
CCATAGGGTCTTCTCGTCTTATTAT2440.1359710225689607No Hit
GTATCAACGCAGAGTACATGGGCAG2360.1315129562552243No Hit
TTGATATACACTGTTCTACAAATCC2330.12984118138757314No Hit
ACGTTAGGGAGTCCGGAGACGTCGG2230.12426859849540262No Hit
GCGTTAGCATCCGCGAGGACCATCG2180.12148230704931737No Hit
GTCGTACTCATAACCGCATCAGGTC2170.1209250487601003No Hit
TATCAACGCAGAGTACATGGGGTGG2030.11312343271106158No Hit
TGCCTACATTGTTCCATCGACCAGA2020.11256617442184454No Hit
GTAGAACAGTGTATATCAATGAGTT1980.11033714126497632No Hit
GTTCTGAGTTGGCTGTTCGACGCCC1950.10866536639732516No Hit
CTGTAGAACATATTAGATGAGTGAG1930.10755084981889104No Hit
CTGTAGAACAGATTAGATGAGTGAG1920.106993591529674No Hit
GATTAGATGAGTGAGTTACACTGAA1900.1058790749512399No Hit
CCCCTTGTCCGTACCAGTTCTGAGT1900.1058790749512399No Hit
GCCTCTGGTCGATGGAACAATGTAG1840.10253552521593758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAG250.006011744519.01
GTAAGAC250.006011744519.03
CTCTAGG250.006011744519.01
CTCAGCC402.7440645E-416.62513
GTCCTAG350.002160146616.2857151
TTATACT350.002160146616.2857154
GGCGAGG859.968062E-1015.647058519
TCCAACG952.9649527E-1015.018
GAACAGT952.9649527E-1015.06
TAGGACC1700.014.5294114
CTTTTTC602.543359E-514.25000117
CTAGAGT400.005255040714.254
GAGTACG400.005255040714.251
GTTATAG400.005255040714.251
ATTTCTA2500.014.0615
TGGACAT2250.013.93333410
TGTAGAA2250.013.9333342
AGTGTAT903.5683115E-813.72222210
CATTTCT4950.013.62626314
GTGGACA2250.013.5111129
Produced by FastQC (version 0.11.5)