FastQCFastQC Report
Thu 2 Feb 2017
SRR4062227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179450
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.30091947617720816No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG5080.28308721092226247No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC4880.2719420451379214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.17609361939258847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.17275006965728615No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA2950.16439119531903038No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.15770409584842576No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGA2820.1571468375592087No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA2760.1538032878239064No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC2480.1382000557258289No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG2400.1337419894120925No Hit
GCGTTAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGT2340.13039843967679018No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC2180.12148230704931737No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA2090.1164669824463639No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA2030.11312343271106158No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGGCTGTTCGACGCCCGGGGAAAGC2020.11256617442184454No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC1890.10532181666202285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1880.1047645583728058No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG1880.1047645583728058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTG207.8392006E-443.99944341
GCTAACG301.2968195E-436.66620639
CGGCGCA250.002347620435.19955433
CTTTTAG250.002347620435.19955416
TGCGCCG250.002347620435.1995545
TAGGACC2950.029.8301324
TGGGTCT300.005732507429.33296424
TAACCGG300.005732507429.33296444
TAACAGT300.005732507429.3329646
CGGGAAA602.9221337E-729.33296440
ACCGTCA300.005732507429.3329648
CGCAATA300.005732507429.33296436
TCAAGGT300.005732507429.3329643
TGAGCGA300.005732507429.33296428
CAATACG300.005732507429.33296438
AGGACCT3550.028.5066815
CTTCGGG804.263711E-927.49965337
TGCTAAC407.009182E-427.49965338
TCGGGAA655.884649E-727.0765839
GGACCTG3750.026.9863246
Produced by FastQC (version 0.11.5)