##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062227_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179450
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138222346057397	32.0	32.0	32.0	32.0	32.0
2	31.449646140986346	32.0	32.0	32.0	32.0	32.0
3	31.5871886319309	32.0	32.0	32.0	32.0	32.0
4	31.694973530231263	32.0	32.0	32.0	32.0	32.0
5	31.680724435775982	32.0	32.0	32.0	32.0	32.0
6	35.33141822234606	36.0	36.0	36.0	36.0	36.0
7	35.32766787405963	36.0	36.0	36.0	36.0	36.0
8	35.296734466425185	36.0	36.0	36.0	36.0	36.0
9	35.32480356645305	36.0	36.0	36.0	36.0	36.0
10	35.25949846753971	36.0	36.0	36.0	36.0	36.0
11	35.35989412092505	36.0	36.0	36.0	36.0	36.0
12	35.292343271106155	36.0	36.0	36.0	36.0	36.0
13	35.30264140429089	36.0	36.0	36.0	36.0	36.0
14	35.27721928113681	36.0	36.0	36.0	36.0	36.0
15	35.26478127612148	36.0	36.0	36.0	36.0	36.0
16	35.26701588186124	36.0	36.0	36.0	36.0	36.0
17	35.27215380328782	36.0	36.0	36.0	36.0	36.0
18	35.26097520200613	36.0	36.0	36.0	36.0	36.0
19	35.20063527444971	36.0	36.0	36.0	36.0	36.0
20	35.16382279186403	36.0	36.0	36.0	36.0	36.0
21	35.12439676790192	36.0	36.0	36.0	36.0	36.0
22	35.11122875452772	36.0	36.0	36.0	36.0	36.0
23	35.108002229033154	36.0	36.0	36.0	36.0	36.0
24	35.088966285873504	36.0	36.0	36.0	36.0	36.0
25	35.065299526330456	36.0	36.0	36.0	36.0	36.0
26	35.04211758149903	36.0	36.0	36.0	36.0	36.0
27	35.02554471997771	36.0	36.0	36.0	36.0	36.0
28	34.94528838116467	36.0	36.0	36.0	36.0	36.0
29	34.914276957369744	36.0	36.0	36.0	36.0	36.0
30	34.83504040122597	36.0	36.0	36.0	32.0	36.0
31	34.85465589300641	36.0	36.0	36.0	32.0	36.0
32	34.81306213429925	36.0	36.0	36.0	32.0	36.0
33	34.77958762886598	36.0	36.0	36.0	32.0	36.0
34	34.73830593480078	36.0	36.0	36.0	32.0	36.0
35	34.72305377542491	36.0	36.0	36.0	32.0	36.0
36	34.66030649205907	36.0	36.0	36.0	32.0	36.0
37	34.566352744497074	36.0	36.0	36.0	32.0	36.0
38	34.53431039286709	36.0	36.0	36.0	32.0	36.0
39	34.373491223181944	36.0	36.0	36.0	32.0	36.0
40	34.433591529674004	36.0	36.0	36.0	32.0	36.0
41	34.37826135413764	36.0	36.0	36.0	32.0	36.0
42	34.38473112287545	36.0	36.0	36.0	32.0	36.0
43	34.392098077458904	36.0	36.0	36.0	32.0	36.0
44	34.233390916689885	36.0	36.0	36.0	32.0	36.0
45	34.17364725550293	36.0	36.0	36.0	32.0	36.0
46	33.9605015324603	36.0	36.0	36.0	32.0	36.0
47	33.95310671496239	36.0	36.0	36.0	32.0	36.0
48	33.820234048481474	36.0	36.0	36.0	32.0	36.0
49	33.7629200334355	36.0	36.0	36.0	32.0	36.0
50	33.46027862914461	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	10.0
22	17.0
23	44.0
24	105.0
25	251.0
26	553.0
27	1086.0
28	1980.0
29	2914.0
30	4283.0
31	6222.0
32	8902.0
33	14150.0
34	29434.0
35	109496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96695578496734	20.170460879841244	12.879328413119579	23.983254922071843
2	13.15221934297417	21.360305386051436	44.230265540973555	21.257209730000838
3	20.36890498746169	24.991919754806354	30.360546113123434	24.278629144608527
4	10.928949568124825	17.06603510727222	39.09612705488994	32.90888826971301
5	11.244357759821677	40.38729451100585	35.09556979660072	13.272777932571747
6	29.5358038450822	40.14321538032878	17.79381443298969	12.52716634159933
7	26.191139593201452	33.01365282808582	20.356645305098915	20.43856227361382
8	24.123711340206185	39.952633045416555	19.320144887155198	16.60351072722207
9	26.266369462245752	14.20618556701031	20.24407913067707	39.28336584006687
10	15.316244079130678	29.067149623850653	32.72945110058512	22.887155196433547
11	35.74421844524937	21.511284480356647	25.08721092226247	17.657286152131512
12	23.546949010866534	25.05823349122318	33.223739203120644	18.171078294789638
13	31.121203677904706	21.597102256896072	26.49205906937866	20.789634995820563
14	20.0261911395932	23.774310392867093	27.196433546949013	29.003064920590692
15	23.705767623293397	33.174700473669546	23.081638339370297	20.03789356366676
16	20.421844524937306	27.761493452215102	31.164112566174424	20.65254945667317
17	19.278907773753133	28.615213151295627	29.553636110337138	22.5522429646141
18	19.129562552242966	27.84675397046531	33.80105879074951	19.22262468654221
19	24.69545834494288	24.51992198383951	27.66731680133742	23.11730286988019
20	23.3006408470326	28.229590415157425	28.87489551407077	19.594873223739203
21	23.623293396489274	24.738367233212593	27.760936193925883	23.877403176372248
22	22.832543884090274	28.818055168570634	28.27807188631931	20.07132906101978
23	22.985789913624966	28.005572582892167	28.425745332961828	20.582892170521035
24	23.359710225689607	27.583728057954858	28.92114795207579	20.135413764279743
25	22.954154105066063	27.919353130973146	29.696459718360092	19.4300330456007
26	18.59414095369715	29.0862584912705	32.73297705754838	19.586623497483966
27	20.410813100100864	28.312779675562417	31.264036021376544	20.01237120296017
28	19.67656188178125	29.652209287421915	28.446282189170063	22.224946641626776
29	20.634274919336413	26.82321996779029	27.661898366666854	24.880606746206443
30	19.998439668095113	28.4504232400292	29.440676738237602	22.110460353638082
31	22.685933372713794	26.33828561238478	28.984206948051224	21.991574066850195
32	20.345169631313805	28.996143729659845	29.712785876688514	20.945900762337835
33	21.266209340815497	28.016316613633958	30.761943504839817	19.955530540710733
34	20.63817574909863	28.61983070398832	31.48582605642829	19.256167490484764
35	20.166062970186683	29.65394260239621	30.140986347171918	20.039008080245193
36	19.19263969150009	29.16427508651483	30.156757630301644	21.486327591683434
37	21.1639091207989	27.291623710622076	27.846662245676995	23.697804922902026
38	21.41376427974366	29.67901922541098	27.932571747004737	20.974644747840625
39	22.667848067406716	25.9924880745397	29.918416477196736	21.42124738085685
40	24.157704095848427	28.207300083588745	28.73780997492338	18.897185845639452
41	22.821525893150703	26.221934923014338	31.803465051351637	19.153074132483326
42	21.40727489564699	30.821058967114173	28.87109268330742	18.90057345393142
43	19.743764071242282	29.766389514277435	30.12026035977798	20.369586054702303
44	19.387106841131498	30.225028421123024	29.33004168431377	21.05782305343171
45	19.562988308460483	30.285434058156774	29.36315103150808	20.78842660187466
46	21.16215176964273	27.423192880190367	28.103074513912496	23.311580836254407
47	22.829454772413175	28.43776470063751	27.539454326601575	21.193326200347734
48	20.827226473030432	28.45861917154751	26.773404367960858	23.9407499874612
49	22.67628160474357	28.02338346995982	28.082455152889775	21.217879772406835
50	20.41015352893644	30.68015269302572	26.16233386274346	22.74735991529438
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	491.0
1	266.0
2	41.0
3	102.5
4	164.0
5	159.5
6	155.0
7	107.5
8	60.0
9	102.0
10	144.0
11	222.5
12	301.0
13	585.5
14	870.0
15	1107.5
16	1345.0
17	1449.5
18	1554.0
19	1452.0
20	1350.0
21	1460.5
22	1571.0
23	1569.5
24	1568.0
25	2116.0
26	2664.0
27	3910.5
28	5157.0
29	6114.0
30	7071.0
31	7506.0
32	7941.0
33	9371.5
34	10802.0
35	12582.0
36	14362.0
37	14720.5
38	15079.0
39	13786.0
40	12493.0
41	11579.5
42	10666.0
43	9373.0
44	8080.0
45	7770.5
46	7461.0
47	7805.0
48	8149.0
49	8212.5
50	8276.0
51	8894.0
52	9512.0
53	8783.5
54	8055.0
55	7375.0
56	6695.0
57	6192.5
58	5690.0
59	5316.5
60	4943.0
61	4644.5
62	4346.0
63	3697.0
64	3048.0
65	2841.5
66	2635.0
67	2481.5
68	2328.0
69	2290.0
70	2252.0
71	1682.5
72	1113.0
73	846.5
74	580.0
75	405.5
76	231.0
77	171.0
78	111.0
79	77.0
80	43.0
81	32.5
82	22.0
83	18.5
84	15.0
85	12.0
86	9.0
87	5.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030091947617720816
2	0.0027862914460852605
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	5.572582892170521E-4
26	5.572582892170521E-4
27	5.572582892170521E-4
28	0.0016717748676511562
29	5.572582892170521E-4
30	5.572582892170521E-4
31	0.0022290331568682086
32	0.0011145165784341043
33	5.572582892170521E-4
34	5.572582892170521E-4
35	0.0
36	5.572582892170521E-4
37	0.0016717748676511562
38	0.0
39	0.0011145165784341043
40	0.0
41	5.572582892170521E-4
42	0.006129841181387573
43	0.0033435497353023124
44	0.0033435497353023124
45	0.0022290331568682086
46	0.0039008080245193644
47	0.0011145165784341043
48	0.0039008080245193644
49	0.0039008080245193644
50	0.0027862914460852605
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	179450.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.33296182780719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.76938369781311	35.40596266369462
2	14.963627326947407	14.764001114516578
3	5.358756551599494	7.930899972137086
4	2.5099403578528827	4.952911674561159
5	1.408593891198265	3.4745054332683196
6	0.806524489427074	2.387294511005851
7	0.5806072654979215	2.0050153246029536
8	0.3637267305259353	1.435497353023126
9	0.35130128320983195	1.559765951518529
>10	1.6842129043918308	15.48230704931736
>50	0.13780950659678295	4.77514628030092
>100	0.06325682270016265	5.242685984954027
>500	0.0022591722392915235	0.5840066870994706
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.30091947617720816	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	508	0.28308721092226247	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	488	0.2719420451379214	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.17609361939258847	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	310	0.17275006965728615	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	295	0.16439119531903038	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	283	0.15770409584842576	No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGA	282	0.1571468375592087	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	276	0.1538032878239064	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	248	0.1382000557258289	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	240	0.1337419894120925	No Hit
GCGTTAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTAATTAAACAGT	234	0.13039843967679018	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	218	0.12148230704931737	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	209	0.1164669824463639	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	203	0.11312343271106158	No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGGCTGTTCGACGCCCGGGGAAAGC	202	0.11256617442184454	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC	189	0.10532181666202285	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	188	0.1047645583728058	No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG	188	0.1047645583728058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	5.572582892170521E-4	0.0
23	0.0	0.0	0.0	5.572582892170521E-4	0.0
24	0.0	0.0	0.0	0.0011145165784341043	0.0
25	0.0	0.0	0.0	0.0033435497353023124	0.0
26	0.0	0.0	0.0	0.006129841181387573	0.0
27	0.0	0.0	0.0	0.006129841181387573	0.0
28	0.0	0.0	0.0	0.006129841181387573	0.0
29	0.0	0.0	0.0	0.008358874338255782	0.0
30	0.0	0.0	0.0	0.017275006965728614	0.0
31	0.0	0.0	0.0	0.0267483978824185	0.0
32	0.0	0.0	0.0	0.03677904708832544	0.0
33	0.0	0.0	0.0	0.042908888269713015	0.0
34	0.0	0.0	0.0	0.05684034550013931	0.0
35	0.0	0.0	0.0	0.07467261075508498	0.0
36	0.0	0.0	0.0	0.09640568403455001	0.0
37	0.0	0.0	0.0	0.1270548899414879	0.0
38	0.0	0.0	0.0	0.1476734466425188	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGTG	20	7.8392006E-4	43.999443	41
GCTAACG	30	1.2968195E-4	36.666206	39
CGGCGCA	25	0.0023476204	35.199554	33
CTTTTAG	25	0.0023476204	35.199554	16
TGCGCCG	25	0.0023476204	35.199554	5
TAGGACC	295	0.0	29.830132	4
TGGGTCT	30	0.0057325074	29.332964	24
TAACCGG	30	0.0057325074	29.332964	44
TAACAGT	30	0.0057325074	29.332964	6
CGGGAAA	60	2.9221337E-7	29.332964	40
ACCGTCA	30	0.0057325074	29.332964	8
CGCAATA	30	0.0057325074	29.332964	36
TCAAGGT	30	0.0057325074	29.332964	3
TGAGCGA	30	0.0057325074	29.332964	28
CAATACG	30	0.0057325074	29.332964	38
AGGACCT	355	0.0	28.506681	5
CTTCGGG	80	4.263711E-9	27.499653	37
TGCTAAC	40	7.009182E-4	27.499653	38
TCGGGAA	65	5.884649E-7	27.07658	39
GGACCTG	375	0.0	26.986324	6
>>END_MODULE