FastQCFastQC Report
Thu 2 Feb 2017
SRR4062226_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062226_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400539
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26840.19164050412019945No Hit
TATCAACGCAGAGTACTTTTTTTTT18650.13316301795237406No Hit
GCGCAAGACGGACCAGAGCGAAAGC15030.10731582626403122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT456.754496E-414.7798646
CGTCTTA759.674823E-713.93280915
CGTCGTA2000.012.82726910
CGGTCCA2250.012.66890710
ACCGTCC751.4761876E-512.6684558
GGTATCA8000.012.464271
TCGCGTA1405.2750693E-1112.2168849
GTATCAA17400.012.1709121
GGTAGCG550.003064939512.0926167
CGAACGA550.003070691412.08958816
CGCGTAA1459.640644E-1111.79519110
ACGGTAT1950.011.6947959
CGTTATT1552.5465852E-1111.6422252
CGAACGT907.45911E-611.6110894
ATCCCGA907.468023E-611.60984316
CCGTCAA2050.011.5849318
GGGTAGG3900.011.4446041
CCGTCGT2250.011.4024249
CTAGGGT1001.9289764E-611.3999784
CGGAATT752.0742565E-411.39957115
Produced by FastQC (version 0.11.5)